FastQCFastQC Report
Thu 26 May 2016
SRR1660616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21367753
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7884433.689873240298126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA597880.2798048068039723No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271920.1272571804812607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1032150.082.064938
AGTGGTA1057500.080.5435645
GTGGTAT1054600.080.517296
TGGTATC1058050.079.981267
GTATCAA1058800.079.956119
AAGCAGT1107250.077.8782651
GCAGTGG1121050.076.546313
CAGTGGT1130350.075.51644
AGCAGTG1170250.073.661112
CAACGCA1010800.043.55550412-13
ATCAACG1020150.043.10459510-11
ACGCAGA1039300.042.33790214-15
TCAACGC1005700.042.01447712-13
AGAGTAC1042100.041.57393618-19
AACGCAG1029850.041.06289314-15
GAGTACT992300.041.0078120-21
CGCAGAG1051150.040.01428216-17
AGTACTT1075150.039.5283120-21
TATCAAC1070650.039.4538810-11
GTACTTT1043750.038.9108922-23
Produced by FastQC (version 0.11.5)