FastQCFastQC Report
Thu 26 May 2016
SRR1660616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21367753
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7157703.3497672871827No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA701150.32813464288921723No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281450.13171717213316722No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA958150.080.534618
AGTGGTA980350.079.053825
GTGGTAT980900.078.884196
GTATCAA977600.078.8025369
TGGTATC984100.078.391867
AAGCAGT1024500.076.371431
GCAGTGG1042000.074.841113
CAGTGGT1057650.073.542634
AGCAGTG1090650.071.765542
CAACGCA934850.043.0478212-13
ATCAACG944050.042.59844210-11
ACGCAGA962750.041.8076414-15
TCAACGC930450.041.2463912-13
AGAGTAC966200.041.0650118-19
GAGTACT915700.040.25580620-21
AACGCAG953300.040.17394314-15
CGCAGAG967100.039.47920616-17
TATCAAC991050.038.8594210-11
AGTACTT1003300.038.68237320-21
GTACTTT965450.038.19844422-23
Produced by FastQC (version 0.11.5)