FastQCFastQC Report
Thu 26 May 2016
SRR1660615_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660615_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21617577
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6821023.1553119944941104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA552900.25576409419057466No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284550.13162899801397723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA907900.081.888918
AGTGGTA927750.080.54145
GTGGTAT930550.080.0272456
GTATCAA930850.079.743929
TGGTATC931850.079.7353447
AAGCAGT1013500.074.775851
GCAGTGG1015200.074.328823
CAGTGGT1018550.073.663864
AGCAGTG1081100.070.095042
CAACGCA883250.043.8322812-13
ATCAACG899250.043.0068710-11
ACGCAGA908100.042.60380614-15
TCAACGC878400.042.1668612-13
AGAGTAC923550.041.33238618-19
AACGCAG896200.041.30261214-15
GAGTACT882850.040.5232220-21
CGCAGAG924700.039.93181616-17
AGTACTT942150.039.82064420-21
TATCAAC945750.039.2102310-11
GTACTTT943350.037.9574322-23
Produced by FastQC (version 0.11.5)