FastQCFastQC Report
Thu 26 May 2016
SRR1660615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21617577
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6102442.8229065634876656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA828800.3833917186926176No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292030.13508914528210075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA821450.080.980578
AGTGGTA839550.079.6175165
GTATCAA839050.079.11399
GTGGTAT847050.078.762416
TGGTATC847200.078.552697
AAGCAGT922000.073.186331
GCAGTGG923600.072.830043
CAGTGGT930500.072.153624
AGCAGTG993650.067.988912
CAACGCA791450.043.5955212-13
ATCAACG805800.042.7695910-11
ACGCAGA820400.042.08577714-15
TCAACGC788700.042.04350712-13
AGAGTAC827350.041.12166618-19
AACGCAG808250.040.921914-15
GAGTACT791350.040.34691220-21
CGCAGAG834750.039.54679516-17
AGTACTT854000.039.0845720-21
TATCAAC851250.039.01762410-11
GTACTTT850000.037.52980422-23
Produced by FastQC (version 0.11.5)