FastQCFastQC Report
Thu 26 May 2016
SRR1660614_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660614_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27687797
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8112832.930110329832308No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA358640.12952998752482908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1135550.078.531958
GTGGTAT1142050.078.253946
AGTGGTA1146800.078.085075
TGGTATC1147500.077.613917
GTATCAA1146950.077.600129
AAGCAGT1236650.073.341211
CAGTGGT1259600.071.355224
GCAGTGG1269850.071.156693
AGCAGTG1323950.068.546392
CAACGCA1097200.042.40810812-13
ATCAACG1113100.041.86326610-11
ACGCAGA1141450.040.8430214-15
AGAGTAC1124050.040.7630318-19
TCAACGC1095450.040.47179412-13
GAGTACT1051800.040.3335120-21
AGTACTT1124100.039.65516720-21
AACGCAG1117650.039.60639614-15
GTACTTT1119800.037.85309222-23
TATCAAC1178500.037.65248510-11
CGCAGAG1185300.037.27836616-17
Produced by FastQC (version 0.11.5)