FastQCFastQC Report
Thu 26 May 2016
SRR1660614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27687797
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7582402.738534958198372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA419760.15160469429908058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1113250.078.094478
GTGGTAT1121950.077.841576
AGTGGTA1126100.077.696675
TGGTATC1126600.077.252737
GTATCAA1127450.076.8858959
AAGCAGT1206350.073.221251
CAGTGGT1237600.070.962374
GCAGTGG1257650.069.995413
AGCAGTG1303400.067.794422
CAACGCA1082350.042.30212812-13
ATCAACG1100350.041.58612410-11
AGAGTAC1112650.040.54271318-19
ACGCAGA1131600.040.5115114-15
TCAACGC1080100.040.1374412-13
GAGTACT1048000.039.85678520-21
AGTACTT1121650.039.26250520-21
AACGCAG1107650.039.1015914-15
GTACTTT1111300.037.61055822-23
TATCAAC1167300.037.23518810-11
CGCAGAG1169600.036.964316-17
Produced by FastQC (version 0.11.5)