FastQCFastQC Report
Thu 26 May 2016
SRR1660613_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660613_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23074605
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6393382.770742987799791No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270380.11717643703976731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA879500.079.9066548
AGTGGTA897300.078.773325
GTGGTAT894450.078.7491766
GTATCAA894750.078.435299
TGGTATC898750.078.080617
AAGCAGT979600.073.1351551
GCAGTGG1007300.070.7383
CAGTGGT1002250.070.733594
AGCAGTG1061650.067.473442
CAACGCA855200.042.99611312-13
ATCAACG871750.042.17540710-11
ACGCAGA893100.041.15514414-15
TCAACGC853450.040.99435812-13
AGAGTAC896800.040.52898818-19
AACGCAG874100.040.01188714-15
GAGTACT850050.039.6282420-21
AGTACTT909600.039.0925720-21
CGCAGAG910550.038.30449316-17
TATCAAC921600.037.9996110-11
GTACTTT900400.037.40781822-23
Produced by FastQC (version 0.11.5)