FastQCFastQC Report
Thu 26 May 2016
SRR1660613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23074605
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6201852.687738316647241No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA342720.1485269195290667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA838700.079.100738
AGTGGTA853150.078.049425
GTGGTAT856300.077.707436
TGGTATC854200.077.638237
GTATCAA857000.077.280059
AAGCAGT940650.071.735521
GCAGTGG953500.070.37243
CAGTGGT956150.070.054494
AGCAGTG1022050.066.020272
CAACGCA811700.042.76480512-13
ATCAACG827550.041.97696710-11
ACGCAGA849850.040.91419614-15
TCAACGC812350.040.59319312-13
AGAGTAC856600.040.22699418-19
AACGCAG833600.039.4710214-15
GAGTACT812500.039.0993420-21
AGTACTT873850.038.48109420-21
CGCAGAG868950.037.80850616-17
TATCAAC882950.037.51503410-11
GTACTTT866050.036.65731422-23
Produced by FastQC (version 0.11.5)