FastQCFastQC Report
Thu 26 May 2016
SRR1660612_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660612_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21754063
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7452283.4256956964774807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA367050.16872710169130245No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248030.11401548299276325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1005250.080.691928
AGTGGTA1017450.080.137345
GTGGTAT1023400.079.3980946
TGGTATC1027550.078.923037
GTATCAA1028600.078.801849
AAGCAGT1076300.076.685021
GCAGTGG1086600.075.6057053
CAGTGGT1104100.074.054134
AGCAGTG1144750.072.050232
CAACGCA979050.043.27584512-13
ATCAACG990950.042.7304810-11
ACGCAGA1004800.042.21797614-15
TCAACGC973350.041.4601312-13
AGAGTAC1018250.041.13583818-19
AACGCAG992100.040.6620714-15
GAGTACT965600.040.3361220-21
AGTACTT1028000.039.9172820-21
CGCAGAG1014350.039.6405816-17
TATCAAC1034450.039.03447310-11
GTACTTT1021900.038.0806622-23
Produced by FastQC (version 0.11.5)