FastQCFastQC Report
Thu 26 May 2016
SRR1660612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21754063
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6851393.1494760312131116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA479310.22033125490167055No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261720.12030856029055353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA931400.079.861588
AGTGGTA945850.078.892285
GTGGTAT949600.078.476456
TGGTATC953000.078.007487
GTATCAA956050.077.640299
AAGCAGT1005550.074.8490451
GCAGTGG1013800.073.966073
CAGTGGT1028150.072.874314
AGCAGTG1080300.069.76532
CAACGCA902950.043.122312-13
ATCAACG922300.042.19975310-11
ACGCAGA933400.041.70797714-15
TCAACGC901250.041.1154112-13
AGAGTAC944350.040.76407618-19
AACGCAG918200.040.28988614-15
GAGTACT893300.040.00576420-21
AGTACTT956400.039.29482320-21
CGCAGAG942700.039.1256416-17
TATCAAC965950.038.42957710-11
GTACTTT953800.037.44600722-23
Produced by FastQC (version 0.11.5)