FastQCFastQC Report
Thu 26 May 2016
SRR1660611_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660611_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31017867
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10027553.232830291006148No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400600.12915136943491312No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336920.10862126657516456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1336700.080.994088
AGTGGTA1355800.080.298835
TGGTATC1363300.079.370237
GTGGTAT1371850.079.093456
GTATCAA1367700.079.004229
AAGCAGT1454450.075.661671
GCAGTGG1484100.073.995323
CAGTGGT1503050.072.653824
AGCAGTG1576550.069.9525762
CAACGCA1298150.043.6479212-13
ATCAACG1318750.042.97469310-11
ACGCAGA1337050.042.34520714-15
TCAACGC1292750.041.7284612-13
AGAGTAC1345700.041.5531518-19
AACGCAG1321800.040.7592314-15
GAGTACT1284350.040.41385320-21
CGCAGAG1361200.039.45141616-17
AGTACTT1387600.039.41434520-21
TATCAAC1380200.039.06836310-11
GAGTGTA367400.038.48984594
Produced by FastQC (version 0.11.5)