FastQCFastQC Report
Thu 26 May 2016
SRR1660611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31017867
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9096632.9327064946148615No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA542120.17477668596618845No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317470.10235068710559626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1285800.080.717488
AGTGGTA1311850.079.6786045
TGGTATC1314550.079.0275657
GTGGTAT1327400.078.6778266
GTATCAA1317000.078.6447759
AAGCAGT1403700.075.014821
GCAGTGG1441250.073.1473163
CAGTGGT1455250.072.2854464
AGCAGTG1536800.068.633042
CAACGCA1248100.043.4737412-13
ATCAACG1267300.042.80672510-11
ACGCAGA1291400.042.0679314-15
TCAACGC1244500.041.53861212-13
AGAGTAC1304700.041.23021318-19
AACGCAG1279450.040.4011314-15
GAGTACT1249700.039.98174320-21
CGCAGAG1307000.039.36099616-17
AGTACTT1358150.038.85230320-21
TATCAAC1333700.038.83000610-11
GTACTTT1337350.037.52533722-23
Produced by FastQC (version 0.11.5)