FastQCFastQC Report
Thu 26 May 2016
SRR1660610_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660610_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25306967
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7890623.1179635236415333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA406520.16063560678764863No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267290.10561913642199794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1049300.080.879228
AGTGGTA1078050.079.236165
GTGGTAT1073950.079.139576
GTATCAA1075200.078.887729
TGGTATC1075600.078.844967
AAGCAGT1164050.074.379181
GCAGTGG1176100.073.322763
CAGTGGT1182150.072.491144
AGCAGTG1239400.069.8947452
CAACGCA1018500.043.67218812-13
ATCAACG1030000.043.10238610-11
ACGCAGA1050600.042.30128514-15
TCAACGC1012700.041.76181412-13
AGAGTAC1068600.041.1024118-19
AACGCAG1043450.040.6002114-15
CGCAGAG1059850.039.81924416-17
GAGTACT1027150.039.8101120-21
TATCAAC1084400.039.0135910-11
AGTACTT1111100.038.75610720-21
GTACTTT1090700.037.50247622-23
Produced by FastQC (version 0.11.5)