FastQCFastQC Report
Thu 26 May 2016
SRR1660610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25306967
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7616983.009835196766171No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465740.18403627744091183No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277770.10976028854030591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1018600.080.364848
GTGGTAT1037200.079.087866
AGTGGTA1041900.078.8622065
GTATCAA1040500.078.5785149
TGGTATC1044350.078.334457
AAGCAGT1126600.073.730091
GCAGTGG1133650.073.043383
CAGTGGT1148450.071.827934
AGCAGTG1202900.069.0883562
CAACGCA987600.043.2803712-13
ATCAACG997500.042.81548710-11
ACGCAGA1019450.041.85674314-15
TCAACGC983200.041.50969712-13
AGAGTAC1034150.040.91642418-19
AACGCAG1011350.040.2311814-15
GAGTACT989650.039.86214420-21
CGCAGAG1028950.039.42657516-17
TATCAAC1049400.038.9336510-11
AGTACTT1079050.038.57586720-21
GTACTTT1057950.037.34643622-23
Produced by FastQC (version 0.11.5)