FastQCFastQC Report
Thu 26 May 2016
SRR1660609_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660609_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21909704
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6465952.951180901394195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA283560.12942210447023839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA886550.079.648378
GTGGTAT898250.078.704226
TGGTATC902750.078.110327
GTATCAA909550.077.526499
AGTGGTA919700.077.2040945
AAGCAGT983100.073.2677461
CAGTGGT985000.072.255984
GCAGTGG1002600.071.5076143
AGCAGTG1060000.067.890152
CAACGCA857900.043.24005512-13
ATCAACG870500.042.60374510-11
ACGCAGA880800.042.1719314-15
GAGTGTA235650.041.8834494
TCAACGC852000.041.32182712-13
AGAGTAC889600.041.26680818-19
AACGCAG869200.040.51229514-15
GAGTACT851400.040.06509820-21
CGCAGAG891450.039.44063616-17
AGTACTT914250.039.3745120-21
TATCAAC917150.038.3664810-11
Produced by FastQC (version 0.11.5)