FastQCFastQC Report
Thu 26 May 2016
SRR1660609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21909704
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6013712.744770079960916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA371790.16969193194029458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA823750.078.797528
GTGGTAT841750.077.3614966
TGGTATC848050.076.551267
GTATCAA852850.075.95469
AGTGGTA861400.075.776965
AAGCAGT907950.072.623991
CAGTGGT921000.071.102874
GCAGTGG935450.070.2004553
AGCAGTG990450.066.658062
CAACGCA794950.042.8655112-13
ATCAACG809250.042.14869310-11
ACGCAGA813700.041.86332714-15
TCAACGC791950.040.82353612-13
AGAGTAC826400.040.733818-19
AACGCAG804150.040.0551814-15
GAGTACT786550.039.64904420-21
AGTACTT850150.038.8385920-21
CGCAGAG827200.038.82826216-17
TATCAAC854300.037.9183410-11
GTACTTT848150.036.8275622-23
Produced by FastQC (version 0.11.5)