FastQCFastQC Report
Thu 26 May 2016
SRR1660608_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660608_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22574345
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7609353.3707954760149184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA351090.15552610718051843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1010000.082.01888
AGTGGTA1026300.081.061655
GTATCAA1025900.080.542029
GTGGTAT1035050.080.073356
TGGTATC1032100.080.049567
AAGCAGT1102650.076.2341
GCAGTGG1111500.075.484373
CAGTGGT1127350.074.006624
AGCAGTG1182200.071.2067952
CAACGCA978050.044.17172212-13
ATCAACG994600.043.4649710-11
ACGCAGA1008450.042.8000214-15
TCAACGC978350.042.1621212-13
AGAGTAC1014000.041.9756618-19
AACGCAG995300.041.40345814-15
GAGTACT972400.040.8113820-21
CGCAGAG1025250.040.0446216-17
AGTACTT1050700.039.7312220-21
TATCAAC1040900.039.65561310-11
GTACTTT1037800.038.22808522-23
Produced by FastQC (version 0.11.5)