FastQCFastQC Report
Thu 26 May 2016
SRR1660608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22574345
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6916343.0638053950181057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA444290.19681191192922762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA931100.080.68318
AGTGGTA942350.079.987545
GTATCAA950950.078.781519
GTGGTAT958100.078.579286
TGGTATC958950.078.306117
AAGCAGT1028100.074.079411
GCAGTGG1028750.073.781363
CAGTGGT1047950.072.2771154
AGCAGTG1104900.069.0025562
CAACGCA899300.043.67527812-13
ATCAACG911750.043.05054510-11
ACGCAGA925300.042.39726314-15
TCAACGC896950.041.6836512-13
AGAGTAC939900.041.27872518-19
AACGCAG913300.040.78307314-15
GAGTACT897750.040.23325320-21
CGCAGAG942700.039.43390716-17
AGTACTT974100.039.1709820-21
TATCAAC963400.038.89146410-11
GTACTTT964450.037.5360422-23
Produced by FastQC (version 0.11.5)