FastQCFastQC Report
Thu 26 May 2016
SRR1660607_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660607_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23366614
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8114653.472753904352595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA558830.23915745773007593No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352300.15077066792818164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1065200.081.4564748
AGTGGTA1081050.080.669175
GTGGTAT1080250.080.455856
TGGTATC1086850.079.7443167
GTATCAA1089100.079.5313649
AAGCAGT1154750.076.48991
GCAGTGG1165550.075.4319843
CAGTGGT1166650.075.0307544
AGCAGTG1255700.070.359332
GAGTGTA276500.043.9896694
CAACGCA1035800.043.6652112-13
ATCAACG1050700.043.02123310-11
ACGCAGA1061050.042.62763214-15
TCAACGC1031650.041.85451512-13
AGAGTAC1075400.041.56509818-19
AACGCAG1055150.040.90609714-15
GAGTACT1033400.040.39554220-21
CGCAGAG1075300.039.96468416-17
AGTACTT1102900.039.73961320-21
TATCAAC1096350.039.40589510-11
Produced by FastQC (version 0.11.5)