FastQCFastQC Report
Thu 26 May 2016
SRR1660607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23366614
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7352333.1465106583264486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA848570.3631548841436761No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352250.15074926987709902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA972100.081.095828
GTGGTAT988100.079.8754046
AGTGGTA992950.079.584995
TGGTATC992200.079.339487
GTATCAA995900.079.0775769
AAGCAGT1063900.074.993111
CAGTGGT1066900.074.3334
GCAGTGG1076750.073.831953
AGCAGTG1160450.068.790122
CAACGCA938650.043.6174312-13
ATCAACG954050.042.9207610-11
ACGCAGA966750.042.3909414-15
TCAACGC937000.041.8637212-13
AGAGTAC979250.041.39155218-19
AACGCAG954400.040.98724414-15
GAGTACT931750.040.5512920-21
CGCAGAG983750.039.7142416-17
AGTACTT1001150.039.5637920-21
TATCAAC1009150.038.98239510-11
GTACTTT994350.038.0196222-23
Produced by FastQC (version 0.11.5)