FastQCFastQC Report
Thu 26 May 2016
SRR1660606_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660606_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32525572
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10353463.1831753796674196No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC914840.281267920514972Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA417110.12824063478422454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1391100.082.95348
AGTGGTA1418050.081.634515
GTATCAA1418600.081.13729
GTGGTAT1426100.081.074166
TGGTATC1426050.080.819517
AAGCAGT1527650.076.987751
GCAGTGG1540300.075.861533
CAGTGGT1542400.075.343684
AGCAGTG1620200.072.54932
CAACGCA1351150.044.2716712-13
ATCAACG1382350.043.41488310-11
ACGCAGA1392000.042.97625414-15
TCAACGC1349150.042.65298512-13
AGAGTAC1397850.042.0435618-19
AACGCAG1372100.041.78033414-15
GAGTACT1343050.040.72024520-21
CGCAGAG1409100.040.5273216-17
AGTACTT1429950.040.15387320-21
TATCAAC1434550.040.14082310-11
GTACTTT1415550.038.569322-23
Produced by FastQC (version 0.11.5)