FastQCFastQC Report
Thu 26 May 2016
SRR1660606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32525572
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9734292.992811317814795No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTGCATCTCGTATG532260.16364354791362318TruSeq Adapter, Index 14 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACTGTTGCATCTCGTATG412230.12674027685047323TruSeq Adapter, Index 4 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA364810.11216097905979948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1292650.082.406218
AGTGGTA1322200.080.941265
GTATCAA1322000.080.420289
GTGGTAT1328300.080.364356
TGGTATC1333200.079.8751147
AAGCAGT1422500.076.236071
GCAGTGG1445150.074.726123
CAGTGGT1450050.074.287614
AGCAGTG1524550.071.2152
CAACGCA1255000.044.1617112-13
ATCAACG1274800.043.40759310-11
ACGCAGA1302350.042.58136714-15
TCAACGC1251600.042.46249812-13
AGAGTAC1310550.041.77235418-19
AACGCAG1277500.041.45998414-15
GAGTACT1263950.040.2261120-21
CGCAGAG1315350.040.15083316-17
TATCAAC1332150.039.8984810-11
AGTACTT1353250.039.5929420-21
GTACTTT1336200.037.96385222-23
Produced by FastQC (version 0.11.5)