FastQCFastQC Report
Thu 26 May 2016
SRR1660605_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660605_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31208817
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9307522.982336690301334No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC671920.21529813193495928Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA438740.14058206692038344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1254500.082.2165158
GTGGTAT1275100.081.036766
GTATCAA1277000.080.5536969
AGTGGTA1290200.080.198445
TGGTATC1286900.080.062637
AAGCAGT1396750.075.382821
GCAGTGG1402550.074.623223
CAGTGGT1405500.073.903534
AGCAGTG1467500.071.697012
CAACGCA1208150.044.38406812-13
ATCAACG1229050.043.69888310-11
ACGCAGA1245150.042.96283714-15
TCAACGC1206300.042.6417812-13
AGAGTAC1262000.041.8212118-19
AACGCAG1233300.041.5533114-15
CGCAGAG1260750.040.46562216-17
GAGTACT1216350.040.17688420-21
TATCAAC1290000.039.842310-11
AGTACTT1319750.039.0596520-21
GTACTTT1287300.037.9685422-23
Produced by FastQC (version 0.11.5)