FastQCFastQC Report
Thu 26 May 2016
SRR1660605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31208817
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9196682.9468210858489123No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG400010.12817211238734233TruSeq Adapter, Index 20 (97% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA375060.12017757674057303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1240600.081.930078
GTGGTAT1263400.080.697016
GTATCAA1260500.080.513579
AGTGGTA1269650.080.4478155
TGGTATC1270950.079.958817
GCAGTGG1369250.074.954013
AAGCAGT1375000.074.938561
CAGTGGT1388650.073.831364
AGCAGTG1450750.071.17582
CAACGCA1187300.044.2981812-13
ATCAACG1207500.043.63301510-11
ACGCAGA1228900.042.78141414-15
TCAACGC1182900.042.6255612-13
AGAGTAC1254100.041.55546618-19
AACGCAG1214000.041.44455314-15
CGCAGAG1242000.040.31346516-17
GAGTACT1205200.040.0726620-21
TATCAAC1272800.039.8419110-11
AGTACTT1306750.038.87731620-21
GTACTTT1274100.037.8075222-23
Produced by FastQC (version 0.11.5)