FastQCFastQC Report
Thu 26 May 2016
SRR1660604_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660604_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28617461
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7221652.5235117818453565No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1152850.4028484567516315Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA472620.16515091957319344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1004150.079.797378
AGTGGTA1018450.078.715625
GTGGTAT1023500.078.294296
GTATCAA1024250.078.112539
TGGTATC1026800.077.873027
AAGCAGT1124250.072.3429951
GCAGTGG1130850.071.603653
CAGTGGT1130500.071.180554
AGCAGTG1208400.067.3012162
CAACGCA961550.043.4290312-13
ATCAACG981350.042.72077610-11
ACGCAGA999100.041.7951214-15
TCAACGC961150.041.57394412-13
AGAGTAC1007800.040.81388518-19
AACGCAG984100.040.42808514-15
GAGTACT956400.039.76083420-21
CGCAGAG1016750.039.0072916-17
TATCAAC1033350.038.72788610-11
AGTACTT1044550.038.43292220-21
GTACTTT1033650.036.75507722-23
Produced by FastQC (version 0.11.5)