FastQCFastQC Report
Thu 26 May 2016
SRR1660604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28617461
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6715792.3467455760663043No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG816400.28528037480334123TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426290.1489615029090107No Hit
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACCACCTCATCTCGTATG350440.12245670571543715TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288140.10068678000469715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA939150.079.134948
AGTGGTA956300.078.1138155
GTGGTAT963400.077.3381356
GTATCAA961750.077.2313849
TGGTATC963650.077.098617
AAGCAGT1053700.071.572841
GCAGTGG1069650.070.328863
CAGTGGT1074250.070.022714
AGCAGTG1145550.065.962242
CAACGCA897050.043.10344712-13
ATCAACG914150.042.2997310-11
TCAACGC895900.041.24672712-13
ACGCAGA943550.041.05144514-15
AGAGTAC954550.040.15275218-19
AACGCAG918350.040.1002414-15
GAGTACT907100.039.12016320-21
CGCAGAG959400.038.3232316-17
TATCAAC977150.037.98779710-11
AGTACTT994500.037.67643720-21
GTACTTT981800.036.12598822-23
Produced by FastQC (version 0.11.5)