FastQCFastQC Report
Thu 26 May 2016
SRR1660603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27596329
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7026682.5462372187257225No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1175380.425918969149846Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316490.11468554386346097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA958600.080.146938
GTGGTAT973800.079.031256
GTATCAA979200.078.2482459
AGTGGTA983850.078.228725
TGGTATC984950.077.936327
AAGCAGT1085250.071.841131
GCAGTGG1093750.071.02963
CAGTGGT1096900.070.495924
AGCAGTG1149700.067.907942
CAACGCA920150.043.21528212-13
ATCAACG940700.042.44045310-11
TCAACGC915800.041.70026412-13
ACGCAGA958400.041.50081614-15
AGAGTAC964050.040.52269418-19
AACGCAG947550.040.19424414-15
GAGTACT921550.039.26630420-21
CGCAGAG974850.038.94005216-17
TATCAAC989400.038.7065410-11
AGTACTT1003000.037.93894620-21
GTACTTT972400.037.18573422-23
Produced by FastQC (version 0.11.5)