FastQCFastQC Report
Thu 26 May 2016
SRR1660603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27596329
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6925912.5097214923042843No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAACATCTCGTATG703400.25488897454440407TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACAAACATCTCGTATG495970.17972317984758046TruSeq Adapter, Index 5 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA306220.11096403438297898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA968750.080.1876148
GTGGTAT987100.078.8445366
TGGTATC991500.078.328747
GTATCAA992550.078.22229
AGTGGTA1000550.078.000735
AAGCAGT1091300.072.119051
GCAGTGG1102600.071.2774353
CAGTGGT1101900.071.107924
AGCAGTG1158050.068.070152
CAACGCA934050.043.11288512-13
ATCAACG953200.042.29850810-11
TCAACGC929200.041.64354312-13
ACGCAGA969000.041.55545814-15
AACGCAG956250.040.39182714-15
AGAGTAC987350.040.28811318-19
GAGTACT942350.039.36926320-21
CGCAGAG986400.039.0047616-17
TATCAAC1004600.038.65834810-11
AGTACTT1020250.038.0952920-21
GTACTTT995950.037.14903622-23
Produced by FastQC (version 0.11.5)