##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27596329
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66539187150581	34.0	31.0	34.0	31.0	34.0
2	32.8858620652044	34.0	31.0	34.0	31.0	34.0
3	32.892143371678166	34.0	31.0	34.0	31.0	34.0
4	36.32709274483574	37.0	37.0	37.0	35.0	37.0
5	36.270798300745	37.0	37.0	37.0	35.0	37.0
6	36.27695172789105	37.0	37.0	37.0	35.0	37.0
7	36.2216273041244	37.0	37.0	37.0	35.0	37.0
8	36.17118845771117	37.0	37.0	37.0	35.0	37.0
9	37.99335607283128	39.0	38.0	39.0	35.0	39.0
10-11	37.938106260437756	39.0	38.0	39.0	35.0	39.0
12-13	37.96418597922934	39.0	38.0	39.0	35.0	39.0
14-15	39.45712560174218	41.0	39.0	41.0	36.0	41.0
16-17	39.40928094095414	41.0	39.0	41.0	36.0	41.0
18-19	39.37343887297473	41.0	39.0	41.0	36.0	41.0
20-21	39.31190266285056	40.5	39.0	41.0	36.0	41.0
22-23	39.18323828506321	40.0	39.0	41.0	35.5	41.0
24-25	39.13768095749257	40.0	39.0	41.0	36.0	41.0
26-27	39.05025847822006	40.0	39.0	41.0	35.5	41.0
28-29	38.955584255427596	40.0	38.5	41.0	35.0	41.0
30-31	38.82238684717811	40.0	38.0	41.0	35.0	41.0
32-33	38.919926632270545	40.0	38.0	41.0	35.0	41.0
34-35	38.9095340905669	40.0	38.0	41.0	35.0	41.0
36-37	38.80823226524079	40.0	38.0	41.0	35.0	41.0
38-39	38.58993063896288	40.0	38.0	41.0	35.0	41.0
40-41	38.488573915030514	40.0	38.0	41.0	34.5	41.0
42-43	38.34544223255202	40.0	38.0	41.0	34.0	41.0
44-45	38.23719223669206	40.0	38.0	41.0	34.0	41.0
46-47	38.1091791230638	40.0	37.0	41.0	33.5	41.0
48-49	37.9794482809652	40.0	37.0	41.0	33.0	41.0
50-51	37.747397869477496	40.0	37.0	41.0	33.0	41.0
52-53	37.558234883342635	40.0	36.5	41.0	33.0	41.0
54-55	37.354921120124345	40.0	36.0	41.0	32.0	41.0
56-57	37.03284587236223	39.0	36.0	41.0	31.5	41.0
58-59	36.74492496809992	39.0	35.0	41.0	31.0	41.0
60-61	36.51518618653952	39.0	35.0	41.0	31.0	41.0
62-63	36.21220699318377	38.0	35.0	40.0	30.5	41.0
64-65	35.90349865012843	37.5	35.0	40.0	30.0	41.0
66-67	35.586339708444555	37.0	35.0	40.0	30.0	41.0
68-69	35.2549046469188	36.5	34.5	39.0	29.0	41.0
70-71	34.88611936391975	36.0	34.0	39.0	29.0	41.0
72-73	34.496368230716485	35.5	34.0	38.5	29.0	40.0
74-75	34.0731417754876	35.0	34.0	37.0	28.0	39.5
76-77	32.47469378988778	34.0	31.5	36.0	25.5	38.5
78-79	33.481055922329375	35.0	33.5	36.0	28.5	39.0
80-81	33.46109946362793	35.0	34.0	36.0	29.0	38.5
82-83	33.208545944643575	35.0	34.0	36.0	28.5	37.0
84-85	32.96617455894224	35.0	33.5	35.0	27.5	37.0
86-87	32.75608746728596	35.0	33.0	35.0	27.0	36.5
88-89	32.566710612125256	35.0	33.0	35.0	27.0	36.0
90-91	32.39242277478283	35.0	33.0	35.0	26.5	36.0
92-93	32.252767696022175	35.0	33.0	35.0	26.0	35.5
94-95	32.14612217081482	35.0	33.0	35.0	25.5	35.0
96-97	32.022084441013874	35.0	33.0	35.0	25.0	35.0
98-99	31.905628009435603	35.0	33.0	35.0	25.0	35.0
100	31.81746901915831	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.29623449893024656
1101	2	0.23831959718364715
1101	3	0.2828744366847147
1101	4	0.16617454508759266
1101	5	0.16689059885622726
1101	6	0.16135023617682265
1101	7	0.175945344815311
1101	8	0.1983054808172895
1101	9	0.2016862413656142
1101	10-11	0.22336556603968205
1101	12-13	0.22761659905342668
1101	14-15	0.2697010235136119
1101	16-17	0.2894217267331669
1101	18-19	0.28774046417238
1101	20-21	0.2751370429583986
1101	22-23	0.2659079675999152
1101	24-25	0.3139660783159215
1101	26-27	0.30095831984706933
1101	28-29	0.29550848798884743
1101	30-31	0.45174255634784544
1101	32-33	0.4050784842875146
1101	34-35	0.3355691435411998
1101	36-37	0.3309109223866642
1101	38-39	0.3227658110662901
1101	40-41	0.3683787144783892
1101	42-43	0.3382124050578952
1101	44-45	0.3397074706555401
1101	46-47	0.3444396321421195
1101	48-49	0.37229134460731217
1101	50-51	0.504566450271156
1101	52-53	0.5708725325864918
1101	54-55	0.40035450663197736
1101	56-57	0.40314444092868484
1101	58-59	0.4559125808224209
1101	60-61	0.35853290101394464
1101	62-63	0.3916824755265438
1101	64-65	0.3698993527867671
1101	66-67	0.3442861277244802
1101	68-69	0.35676747275487486
1101	70-71	0.38430440069555516
1101	72-73	0.39349198205680835
1101	74-75	0.3511209594464191
1101	76-77	0.31606413644533404
1101	78-79	0.4513262952821009
1101	80-81	0.41190148104249147
1101	82-83	0.35720218733494136
1101	84-85	0.3731455412190101
1101	86-87	0.3904653272086449
1101	88-89	0.42558271479068566
1101	90-91	0.3889995622865712
1101	92-93	0.36674187415099624
1101	94-95	0.43258320280918383
1101	96-97	0.4227594555980545
1101	98-99	0.3745815467657714
1101	100	0.46780980198455424
1102	1	0.28132027523610503
1102	2	0.2583390067861515
1102	3	0.27783463692725263
1102	4	0.1662057093286009
1102	5	0.1466180082738333
1102	6	0.15160138650483646
1102	7	0.15062546618437977
1102	8	0.18556114684263036
1102	9	0.2151419929697056
1102	10-11	0.19721204096413913
1102	12-13	0.22179159292159767
1102	14-15	0.27309587234875465
1102	16-17	0.2702503466099486
1102	18-19	0.2664385423315565
1102	20-21	0.25823979208452386
1102	22-23	0.2578892369198087
1102	24-25	0.26062909136803114
1102	26-27	0.3003517922515826
1102	28-29	0.3272730543187734
1102	30-31	0.4571649154497095
1102	32-33	0.4035075889067343
1102	34-35	0.3573185702133017
1102	36-37	0.31609282340895817
1102	38-39	0.32123766909117535
1102	40-41	0.3414258308178404
1102	42-43	0.3753349155983088
1102	44-45	0.39143751770226487
1102	46-47	0.3758253413051662
1102	48-49	0.40391199665460675
1102	50-51	0.5362691467693992
1102	52-53	0.5757196002790295
1102	54-55	0.40428698958562137
1102	56-57	0.4233662981891513
1102	58-59	0.4375584346563741
1102	60-61	0.36644671468068424
1102	62-63	0.42497302247936375
1102	64-65	0.3958906085731826
1102	66-67	0.3968091002606755
1102	68-69	0.33896082918379733
1102	70-71	0.3262151487794256
1102	72-73	0.4068202558474283
1102	74-75	0.3711125922254226
1102	76-77	0.37513820355804484
1102	78-79	0.4197378296965013
1102	80-81	0.3837202568441995
1102	82-83	0.3792978620344698
1102	84-85	0.37484189095589926
1102	86-87	0.37437248906658027
1102	88-89	0.4220825922141387
1102	90-91	0.40359521884992233
1102	92-93	0.3885181003916358
1102	94-95	0.4409433162259191
1102	96-97	0.4357518924240651
1102	98-99	0.4262764486065116
1102	100	0.4574999719927426
1103	1	0.2603767856881447
1103	2	0.21809516369863502
1103	3	0.24307958203709745
1103	4	0.15569360364948182
1103	5	0.11851922991208141
1103	6	0.12743988382708693
1103	7	0.1249829553652404
1103	8	0.18833301006449688
1103	9	0.18930317858112033
1103	10-11	0.176854969925337
1103	12-13	0.1976304082739091
1103	14-15	0.2334977603756201
1103	16-17	0.22904180561021548
1103	18-19	0.2424862070570981
1103	20-21	0.2196541673398258
1103	22-23	0.23464248697976586
1103	24-25	0.28176198130579166
1103	26-27	0.2654196456984721
1103	28-29	0.2756535932386228
1103	30-31	0.40889506255363983
1103	32-33	0.3456198335090832
1103	34-35	0.2739225579748066
1103	36-37	0.28732301126893134
1103	38-39	0.2768242341832803
1103	40-41	0.2533994696342674
1103	42-43	0.2769105241888141
1103	44-45	0.31404283667976784
1103	46-47	0.35696076025646306
1103	48-49	0.2988346844156453
1103	50-51	0.4247105053714364
1103	52-53	0.4982306204540947
1103	54-55	0.37451104889078835
1103	56-57	0.36219113646749435
1103	58-59	0.34212767557202284
1103	60-61	0.23764751367240677
1103	62-63	0.29385992634671965
1103	64-65	0.2656146022388697
1103	66-67	0.2636498970558776
1103	68-69	0.23210446452829814
1103	70-71	0.2539891191913384
1103	72-73	0.28872398240604014
1103	74-75	0.26767491284562794
1103	76-77	0.21471684823692527
1103	78-79	0.316078933429246
1103	80-81	0.27410119883342077
1103	82-83	0.21906277017033204
1103	84-85	0.2582456515629943
1103	86-87	0.2628238781437986
1103	88-89	0.2963708828737168
1103	90-91	0.2989574048720982
1103	92-93	0.3018105664774424
1103	94-95	0.3065387584042085
1103	96-97	0.3018865690161263
1103	98-99	0.31480297469348173
1103	100	0.38816251012816494
1104	1	0.23701791865703115
1104	2	0.18233171295918993
1104	3	0.21386003212605686
1104	4	0.1481010690029123
1104	5	0.13712872163716838
1104	6	0.12733685746640333
1104	7	0.13379543185015308
1104	8	0.1796207946508801
1104	9	0.15077089992154669
1104	10-11	0.16934767504776715
1104	12-13	0.15485885630440066
1104	14-15	0.18275377418041927
1104	16-17	0.1905139878668649
1104	18-19	0.19970137175677394
1104	20-21	0.20130177204321598
1104	22-23	0.23532278130670647
1104	24-25	0.2483082228724811
1104	26-27	0.23214468108202624
1104	28-29	0.2456202050389038
1104	30-31	0.40728637846980575
1104	32-33	0.34082576987473345
1104	34-35	0.2478455882253101
1104	36-37	0.2138400707478354
1104	38-39	0.24502077173167436
1104	40-41	0.23752816487647266
1104	42-43	0.25860272332587897
1104	44-45	0.25542991025170636
1104	46-47	0.2367635689804075
1104	48-49	0.20981991430913638
1104	50-51	0.34797924274766245
1104	52-53	0.42712171032349744
1104	54-55	0.2689951618798503
1104	56-57	0.12584906825638598
1104	58-59	0.25139550795115184
1104	60-61	0.201370006315301
1104	62-63	0.1713679333507443
1104	64-65	0.16806285726762837
1104	66-67	0.2215561042578642
1104	68-69	0.17599453370766582
1104	70-71	0.1801436716935072
1104	72-73	0.2451144391309512
1104	74-75	0.19857996923065002
1104	76-77	0.0748446395866722
1104	78-79	0.22901750335412885
1104	80-81	0.24318495425693243
1104	82-83	0.22250636183864003
1104	84-85	0.19449377726015626
1104	86-87	0.25118451554926224
1104	88-89	0.21927420622132132
1104	90-91	0.25537259086929254
1104	92-93	0.252462788146282
1104	94-95	0.3083086172507308
1104	96-97	0.266036908262997
1104	98-99	0.26765942288805533
1104	100	0.3247435549421276
1105	1	0.22796329510271818
1105	2	0.18791073288252136
1105	3	0.20787945249706752
1105	4	0.10893912528486283
1105	5	0.10935436322555603
1105	6	0.11805367970783465
1105	7	0.12641737390540442
1105	8	0.1604839296975058
1105	9	0.15936805905302975
1105	10-11	0.1729878532727085
1105	12-13	0.16107627968645488
1105	14-15	0.2134663239011303
1105	16-17	0.1733572134067458
1105	18-19	0.17881126373994505
1105	20-21	0.17610904614149803
1105	22-23	0.14982854584150118
1105	24-25	0.18530310925051907
1105	26-27	0.14539218809184007
1105	28-29	0.18690430466511287
1105	30-31	0.2897855807534313
1105	32-33	0.2804345726286357
1105	34-35	0.2171003741919506
1105	36-37	0.1814651837736534
1105	38-39	0.15745949451429198
1105	40-41	0.20237080445868827
1105	42-43	0.18278970881103618
1105	44-45	0.1700298545319825
1105	46-47	0.18980232560532073
1105	48-49	0.19253084912103446
1105	50-51	0.324238531667028
1105	52-53	0.37342532023178165
1105	54-55	0.2350651974331086
1105	56-57	0.22476143582342445
1105	58-59	0.2492768842633737
1105	60-61	0.1653555920638965
1105	62-63	0.1453773409938961
1105	64-65	0.1329144914465914
1105	66-67	0.13296355132325033
1105	68-69	0.13238503239784194
1105	70-71	0.15638145141517157
1105	72-73	0.13166918013508422
1105	74-75	0.13751162230211378
1105	76-77	0.08690949150182092
1105	78-79	0.17884599695219094
1105	80-81	0.18066907584434233
1105	82-83	0.17064690260482251
1105	84-85	0.16900456473798187
1105	86-87	0.17834740368251545
1105	88-89	0.1864413033511667
1105	90-91	0.15597418000546526
1105	92-93	0.16894434133477887
1105	94-95	0.20664059947486635
1105	96-97	0.17529498003227673
1105	98-99	0.13256089732479026
1105	100	0.1842908010772213
1106	1	0.19314000844268264
1106	2	0.14757892066889156
1106	3	0.18251071195371793
1106	4	0.11459051253798691
1106	5	0.1075460065243874
1106	6	0.10257631507275278
1106	7	0.09567541132789614
1106	8	0.18051962882588413
1106	9	0.16165416661817034
1106	10-11	0.13716871672372122
1106	12-13	0.12403358591027569
1106	14-15	0.10278489848864325
1106	16-17	0.14403128012016708
1106	18-19	0.10648555095149703
1106	20-21	0.13153624410808362
1106	22-23	0.11953213503848303
1106	24-25	0.13846393771505916
1106	26-27	0.15718843133830518
1106	28-29	0.16123717374245672
1106	30-31	0.27794821620165067
1106	32-33	0.2204901330328184
1106	34-35	0.1441134432366411
1106	36-37	0.08988280462704523
1106	38-39	0.1198815666780888
1106	40-41	0.14256989427792632
1106	42-43	0.153136022825052
1106	44-45	0.140117541974476
1106	46-47	0.11746288582802578
1106	48-49	0.11760906819398542
1106	50-51	0.27302247917000244
1106	52-53	0.31900745167145317
1106	54-55	0.1949528004626373
1106	56-57	0.13335996753644963
1106	58-59	0.17075583471709166
1106	60-61	0.08554981290482289
1106	62-63	0.11944269642681604
1106	64-65	0.056145000283777335
1106	66-67	0.05422219406704443
1106	68-69	0.038535036584100624
1106	70-71	0.04281111352825917
1106	72-73	0.0544586134253251
1106	74-75	0.06596580369411242
1106	76-77	0.022642674347601144
1106	78-79	0.13357643336389913
1106	80-81	0.10924645524499965
1106	82-83	0.12944302041231026
1106	84-85	0.08419548938530141
1106	86-87	0.09145832092674766
1106	88-89	0.09677611526655738
1106	90-91	0.041909342791718984
1106	92-93	0.08836002385823605
1106	94-95	0.12478419403018393
1106	96-97	0.03966479161093872
1106	98-99	0.0366229599803205
1106	100	0.05829615789813758
1107	1	0.13994574453639785
1107	2	0.10176369826123732
1107	3	0.12844866394224397
1107	4	0.07507639203647898
1107	5	0.060576330111828725
1107	6	0.05050696391342768
1107	7	0.05269367256568813
1107	8	0.09118706825415757
1107	9	0.05936854113528511
1107	10-11	0.06070799374191438
1107	12-13	0.04419374443723001
1107	14-15	-0.015115379497380843
1107	16-17	0.01398506611008088
1107	18-19	0.006531350296896221
1107	20-21	0.03299675739625485
1107	22-23	0.022048561177854253
1107	24-25	0.02692945182199935
1107	26-27	0.005052694176640671
1107	28-29	0.008517545271331528
1107	30-31	0.15512793986869866
1107	32-33	0.0669220658816343
1107	34-35	-0.019805203055675236
1107	36-37	0.03521726890206622
1107	38-39	-0.02125292033532844
1107	40-41	-0.006099065179647312
1107	42-43	0.019034700890294687
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2312	62-63	0.0861857915797728
2312	64-65	0.14325736127815958
2312	66-67	0.09353202617250389
2312	68-69	0.12947580232192024
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2312	78-79	0.10350803644790574
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2312	94-95	0.08700650344619731
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2312	100	0.15908121341849935
2313	1	0.008773162545082869
2313	2	-0.07598161610000886
2313	3	-0.039809917366653735
2313	4	-0.056279261175390616
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2313	10-11	-0.19629428626392809
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2313	54-55	-0.03439016092412572
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2313	64-65	-0.015358696987135545
2313	66-67	-0.047874627814316284
2313	68-69	6.638417381736872E-4
2313	70-71	-0.012212031285940839
2313	72-73	-0.011411294272896555
2313	74-75	8.345281399897431E-4
2313	76-77	0.02918519536716957
2313	78-79	-0.06187324617700085
2313	80-81	-0.06224113464232062
2313	82-83	-0.09086944308564426
2313	84-85	-0.126828886809065
2313	86-87	-0.126881341668458
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2313	92-93	0.034629511779364464
2313	94-95	-0.04420970205717367
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2313	100	0.04639511603114954
2314	1	-0.026195483910726125
2314	2	-0.09343532076173489
2314	3	-0.039015324946113594
2314	4	-0.06791425372684046
2314	5	-0.09684157028994633
2314	6	-0.1003749159271905
2314	7	-0.12321053229297263
2314	8	-0.0788877301307025
2314	9	-0.13683971294381791
2314	10-11	-0.15107152593259343
2314	12-13	-0.136419266922978
2314	14-15	-0.22731878207906675
2314	16-17	-0.22627012751626552
2314	18-19	-0.19176230107657943
2314	20-21	-0.19328319452107223
2314	22-23	-0.20552598473406647
2314	24-25	-0.17542183974669
2314	26-27	-0.5629069237454374
2314	28-29	-0.5066047379023928
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2314	78-79	-0.11751801268999884
2314	80-81	-0.14178883528069264
2314	82-83	-0.11859612872678582
2314	84-85	-0.11134167656395277
2314	86-87	-0.13308146950443245
2314	88-89	-0.10833779957640388
2314	90-91	-0.07349242924708932
2314	92-93	-0.09917934407782525
2314	94-95	-0.1652271899352158
2314	96-97	-0.2064904798549776
2314	98-99	-0.12751679075460842
2314	100	-0.12502537345108422
2315	1	-0.3591422671500979
2315	2	-0.3115179566513291
2315	3	-0.16463597514974992
2315	4	-0.14578865032856925
2315	5	-0.13281292217135388
2315	6	-0.13503925683949092
2315	7	-0.18912201028679476
2315	8	-0.12763034131907602
2315	9	-0.20907224220145793
2315	10-11	-0.3176901162704411
2315	12-13	-0.27108074661675374
2315	14-15	-0.24995767803222435
2315	16-17	-0.2750354705456104
2315	18-19	-0.2715931818311077
2315	20-21	-0.24287009108011404
2315	22-23	-0.242758837059327
2315	24-25	-0.24219109511272308
2315	26-27	-0.2210162539652245
2315	28-29	-0.21929000954619227
2315	30-31	-0.05904264513659996
2315	32-33	-0.10604816016046215
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2315	90-91	-0.07857268329126299
2315	92-93	-0.11162920437752888
2315	94-95	-0.17004134990094855
2315	96-97	-0.16526466070254386
2315	98-99	-0.09815096189685235
2315	100	-0.08948852181778122
2316	1	-0.4628955919946236
2316	2	-0.5057968888872892
2316	3	-0.3666297677820083
2316	4	-0.25570163674063195
2316	5	-0.21434738531611686
2316	6	-0.23267774815187892
2316	7	-0.1947132586737368
2316	8	-0.15416450549761862
2316	9	-0.91710734903603
2316	10-11	-0.535979865721437
2316	12-13	-0.32041432906606815
2316	14-15	-0.36992654352820153
2316	16-17	-0.3165830613938354
2316	18-19	-0.33962123783922493
2316	20-21	-0.35700660049655397
2316	22-23	-0.3703249731401854
2316	24-25	-0.41051581426133055
2316	26-27	-0.6970123307714786
2316	28-29	-0.6412695991428521
2316	30-31	-0.32662575064806276
2316	32-33	-0.32986955062327183
2316	34-35	-0.3854972718869405
2316	36-37	-0.3762090758641037
2316	38-39	-0.44980451846017644
2316	40-41	-0.4366489788187593
2316	42-43	-0.48066439445698705
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2316	50-51	-0.37505144909471255
2316	52-53	-0.3154322366900999
2316	54-55	-0.4576183964711902
2316	56-57	-0.4612694779561224
2316	58-59	-0.45024198618354916
2316	60-61	-0.45945145794804176
2316	62-63	-0.5061508007100954
2316	64-65	-0.5076917832678021
2316	66-67	-0.4802234968650865
2316	68-69	-0.49685605146186873
2316	70-71	-0.49898336689884815
2316	72-73	-0.5221745012522305
2316	74-75	-0.49347066628725145
2316	76-77	-0.46943936812223086
2316	78-79	-0.4758290259550222
2316	80-81	-0.49825536403092485
2316	82-83	-0.4883279985649054
2316	84-85	-0.544043905076073
2316	86-87	-0.583987693617388
2316	88-89	-0.5718114196463304
2316	90-91	-0.5668352910255479
2316	92-93	-0.5204493042390013
2316	94-95	-0.5832673489178504
2316	96-97	-0.6633977017278738
2316	98-99	-0.6696447420928315
2316	100	-0.7438144323028375
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	7.0
10	6.0
11	23.0
12	75.0
13	448.0
14	2109.0
15	6462.0
16	12548.0
17	19177.0
18	25040.0
19	32301.0
20	41177.0
21	57656.0
22	86546.0
23	135268.0
24	215929.0
25	288889.0
26	308190.0
27	289058.0
28	295713.0
29	347792.0
30	426257.0
31	480748.0
32	601119.0
33	788634.0
34	1121562.0
35	1799345.0
36	3362775.0
37	6941581.0
38	8049333.0
39	1857811.0
40	2746.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.609776665302423	31.86679074673675	27.34235401535161	22.181078572609216
2	22.990360356974854	30.005531052622402	26.865615932156672	20.13849265824607
3	24.58248190576758	26.337168577403595	26.51483323011718	22.56551628671164
4	21.786604297984706	26.93165456898271	26.286383960707237	24.995357172325345
5	22.52045552870456	29.022436281289444	25.984800369643366	22.472307820362627
6	25.768825991312106	26.21264589214022	26.094749776319887	21.92377834022779
7	22.68671677308964	26.332462553262065	28.925673411126528	22.055147262521764
8	25.038080246108095	26.77405027313597	26.087129197510293	22.100740283245646
9	24.79439566037932	26.451264586677453	26.491838823924734	22.262500929018493
10-11	22.142925241976933	28.184422645490276	27.641957377736727	22.030694734796068
12-13	22.192551407833992	26.4760921642875	27.51876164398533	23.812594783893175
14-15	21.928788064528437	29.665657703964897	26.061709149793078	22.34384508171359
16-17	23.532405342754103	26.62121472750959	26.079354612709537	23.767025317026768
18-19	22.31601569752295	27.834856727408503	26.27034244790933	23.578785127159215
20-21	23.565516630853327	27.83762833092764	26.221315523524886	22.375539514694147
22-23	23.75106848450749	26.663077179577037	27.49595788628263	22.08989644963285
24-25	22.290535454915037	27.95599008839183	25.914571825839587	23.838902630853546
26-27	22.223618945838773	26.279161985639465	29.08964087216093	22.407578196360827
28-29	22.24184057234569	26.58973227924627	28.82004704321361	22.348380105194426
30-31	22.54390973524051	26.379332917794972	28.941624808140244	22.135132538824276
32-33	22.275797987478697	26.60901020566902	28.706747553270585	22.4084442535817
34-35	22.25421178302375	26.702961107616886	28.693403387095433	22.34942372226393
36-37	22.292676876059673	26.694029924302875	28.645704463917	22.367588735720453
38-39	22.34186166354131	26.926594535491482	28.642770441266087	22.088773359701122
40-41	22.363092820780768	26.811627866610333	28.597524696558942	22.227754616049957
42-43	22.434233915677698	26.61080392250723	28.70118884290733	22.253773318907744
44-45	22.47641865733604	26.673044819399074	28.637303634362116	22.21323288890277
46-47	22.730033440780247	26.808797607679214	28.530853293875637	21.930315657664902
48-49	22.583736043464302	27.07544688445141	28.374699241351784	21.966117830732507
50-51	22.907156406840585	26.920434567063044	27.966788872693883	22.20562015340249
52-53	22.981636569774107	27.043572437053353	27.780553815436228	22.194237177736316
54-55	22.85336174824933	27.211758586033003	27.544642153426413	22.390237512291257
56-57	23.07285647110442	27.487180641182373	27.17393338729356	22.26602950041965
58-59	23.14931152396743	27.750681754067013	26.6079077492667	22.492098972698855
60-61	23.36134066137073	27.89241208661612	26.15037133312009	22.595875918893064
62-63	23.121771083460672	27.975643812003675	26.49996499485577	22.402620109679887
64-65	23.28188250980798	28.329346051687065	25.944548734845174	22.444222703659783
66-67	22.982629819579767	28.52650704324265	25.911681443886575	22.579181693291
68-69	22.857377414997263	28.49902235810024	25.972820547794	22.670779679108495
70-71	22.752742554903868	28.561209316050352	25.976321389180534	22.709726739865243
72-73	22.66532081132965	28.581107653847727	25.899437566496612	22.854133968326003
74-75	22.604792434456495	28.62048149668304	25.76578952570386	23.008936543156608
76-77	22.561558627129337	28.672396914176236	25.65753236721726	23.108512091477166
78-79	22.539855147891565	28.724134292979414	25.433342481326832	23.302668077802192
80-81	22.486465500538134	28.813207365370953	25.258538916534878	23.441788217556038
82-83	22.406587287070813	28.93466443771095	25.10392084761196	23.554827427606273
84-85	22.342607988801767	28.950180350052097	24.973678944655955	23.73353271649018
86-87	22.27998941843041	28.931924631683465	24.914205938362358	23.873880011523767
88-89	22.198006902755278	28.969318492398006	24.86708447877772	23.965590126068996
90-91	22.14284558521347	28.913843890805964	24.81395319298233	24.12935733099824
92-93	22.080340951839588	28.88560905319208	24.824126224483035	24.209923770485297
94-95	22.022181687729418	28.873443712627918	24.84081874682584	24.263555852816822
96-97	21.95178972101022	28.849235384426585	24.82912297273692	24.36985192182628
98-99	21.87076094530852	28.831335403918025	24.87357438939675	24.42432926137671
100	21.809581321180318	28.871967528885605	24.859299979119584	24.459151170814497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37261.0
1	22769.0
2	6480.5
3	4332.5
4	3942.5
5	3902.5
6	4019.5
7	4196.0
8	4289.5
9	4419.0
10	4891.0
11	6806.0
12	50066.0
13	60498.0
14	28749.0
15	30377.5
16	34915.5
17	40002.0
18	45975.5
19	52391.0
20	60257.0
21	71031.0
22	85343.5
23	102963.0
24	123328.5
25	147595.5
26	175860.0
27	208789.5
28	247462.5
29	286739.0
30	321816.0
31	356228.0
32	393097.5
33	431273.0
34	470371.0
35	514412.0
36	567551.5
37	616090.5
38	660575.0
39	705224.0
40	742148.0
41	782142.5
42	830176.5
43	872861.5
44	915038.5
45	959134.0
46	995205.0
47	1027627.0
48	1058211.0
49	1083354.0
50	1100969.0
51	1103741.5
52	1086778.5
53	1055028.5
54	1008662.0
55	950753.5
56	885373.5
57	806860.0
58	711676.0
59	606463.5
60	496651.0
61	394698.5
62	307205.5
63	231381.0
64	169560.5
65	121745.5
66	86774.5
67	62244.0
68	44495.0
69	32376.5
70	24053.0
71	17810.0
72	13502.5
73	10125.0
74	7425.5
75	5593.5
76	4173.5
77	2671.0
78	1578.5
79	976.0
80	563.5
81	371.0
82	218.0
83	99.5
84	56.0
85	38.5
86	31.5
87	20.0
88	9.5
89	4.5
90	3.0
91	3.5
92	2.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0746005021175099
2	0.004420877863863705
3	0.0018806849273321827
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.8118351901080756E-6
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0020473737648221257
38-39	1.1595745216691684E-4
40-41	0.00100738036570009
42-43	0.0
44-45	1.5219415596907836E-4
46-47	0.0019259808070848845
48-49	2.6633977294588713E-4
50-51	1.3045213368778144E-4
52-53	1.5038232077897027E-4
54-55	0.0017900931678267787
56-57	2.808344544667517E-4
58-59	8.479388689705794E-4
60-61	7.89960142887121E-4
62-63	0.0013951130963832182
64-65	1.5400599115918644E-4
66-67	0.002130718183567097
68-69	0.0012139295773724106
70-71	0.0024079289676536327
72-73	0.0
74-75	0.0017375499473136443
76-77	7.138630649025818E-4
78-79	4.112865881545332E-4
80-81	0.0
82-83	0.0016179688247665116
84-85	0.0019966423794990995
86-87	0.004272307378274842
88-89	0.011394631510589687
90-91	0.006259890581823402
92-93	0.00402952146280036
94-95	0.005252510216123311
96-97	0.0019332281478453167
98-99	9.385306284759832E-4
100	0.003967919066336686
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.7596329E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.983260705351135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38900001078558	35.21443520422542
2	13.201763848464026	12.669273530226583
3	4.797675650763803	6.9062436459094405
4	2.5121551846980528	4.821655886386648
5	1.4796903471121172	3.5500183844336117
6	0.9441073270022076	2.7180808803227476
7	0.6557290008300376	2.202481091922098
8	0.4702966948736843	1.8053095135191175
9	0.3587541097620422	1.5492772780045405
>10	2.012168891843119	17.486660182100884
>50	0.13458617891155727	4.44116148066382
>100	0.04322529136051789	3.0701312161014993
>500	5.743315366969479E-4	0.18841598098247908
>1k	2.2785295824889127E-4	0.19959239461293057
>5k	0.0	0.0
>10k+	4.5279098333718777E-5	3.177263330588092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	692591	2.5097214923042843	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAACATCTCGTATG	70340	0.25488897454440407	TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACAAACATCTCGTATG	49597	0.17972317984758046	TruSeq Adapter, Index 5 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30622	0.11096403438297898	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.4677941040636238	1.8118351901080756E-5	0.0	3.6236703802161512E-6	0.0
2	0.4678122224155249	2.1742022281296907E-5	0.0	3.6236703802161512E-6	0.0
3	0.4678122224155249	2.1742022281296907E-5	0.0	3.6236703802161512E-6	0.0
4	0.4678122224155249	3.623670380216151E-5	0.0	3.6236703802161512E-6	0.0
5	0.4678122224155249	5.073138532302612E-5	0.0	3.6236703802161512E-6	0.0
6	0.4678158460859051	5.435505570324227E-5	0.0	7.2473407604323024E-6	3.6236703802161512E-6
7	0.4678158460859051	5.797872608345842E-5	0.0	7.2473407604323024E-6	3.6236703802161512E-6
8	0.4678158460859051	6.884973722410688E-5	0.0	7.2473407604323024E-6	3.6236703802161512E-6
9	0.46782671709704576	7.247340760432302E-5	0.0	7.2473407604323024E-6	1.0871011140648454E-5
10-11	0.46783758810818643	9.240359469551186E-5	0.0	1.8118351901080756E-5	1.0871011140648454E-5
12-13	0.46786114196565787	1.0689827621637646E-4	0.0	2.1742022281296907E-5	2.536569266151306E-5
14-15	0.4679408627140226	1.1776928735702491E-4	0.0	2.3553857471404983E-5	2.898936304172921E-5
16-17	0.46799884144010606	1.3226396887788952E-4	0.0	3.080119823183728E-5	2.898936304172921E-5
18-19	0.4680405136494785	1.5400599115918644E-4	0.0	3.623670380216151E-5	2.898936304172921E-5
20-21	0.4681184225626532	2.0473737648221257E-4	0.0	3.623670380216151E-5	3.623670380216151E-5
22-23	0.4685985588880318	2.246675635734014E-4	0.0	3.623670380216151E-5	4.3484044562593815E-5
24-25	0.4710264180427766	2.3916224509426597E-4	0.0	3.623670380216151E-5	4.7107714942809966E-5
26-27	0.4798917276279755	2.609042673755629E-4	0.0	3.623670380216151E-5	4.7107714942809966E-5
28-29	0.5050327527259151	2.8808179522718405E-4	0.0	3.8048538992269584E-5	5.073138532302612E-5
30-31	0.5580053781791049	3.043883119381567E-4	0.0	3.986037418237766E-5	5.073138532302612E-5
32-33	0.6541486007069999	3.206948286491294E-4	0.0	4.8919550132918045E-5	5.073138532302612E-5
34-35	0.8029165763315838	3.4062501574031823E-4	0.0	5.797872608345842E-5	5.616689089335035E-5
36-37	1.0086631450146866	3.6236703802161515E-4	0.0	6.341423165378265E-5	6.522606684389072E-5
38-39	1.2761425622951517	3.659907084018313E-4	0.0	7.066157241421495E-5	6.522606684389072E-5
40-41	1.6045521851837612	3.786735547325878E-4	0.0	7.42852427944311E-5	6.522606684389072E-5
42-43	1.9918029677063207	4.040392473941009E-4	0.0	8.334441874497148E-5	6.522606684389072E-5
44-45	2.4348727687657297	4.2759310486550586E-4	0.0	8.334441874497148E-5	7.066157241421495E-5
46-47	2.9366170406215986	4.457114567665866E-4	0.0	8.334441874497148E-5	7.609707798453917E-5
48-49	3.4900185455826387	4.602061382874512E-4	0.0	8.515625393507956E-5	9.602726507572802E-5
50-51	4.098023327667966	4.728889846182077E-4	3.6236703802161512E-6	9.240359469551186E-5	1.0146277064605223E-4
52-53	4.751990744855956	4.873836661390724E-4	3.6236703802161512E-6	1.0146277064605223E-4	1.0871011140648455E-4
54-55	5.449610707279218	4.946310068995047E-4	3.6236703802161512E-6	1.032746058361603E-4	1.1414561697680877E-4
56-57	6.1830126028719254	5.109375236104774E-4	3.6236703802161512E-6	1.0689827621637646E-4	1.3045213368778144E-4
58-59	6.952348625790046	5.326795458917743E-4	3.6236703802161512E-6	1.1052194659659262E-4	1.340758040679976E-4
60-61	7.756808885703602	5.634807441236116E-4	3.6236703802161512E-6	1.1776928735702491E-4	1.3951130963832183E-4
62-63	8.589591753308927	5.779754256444761E-4	3.6236703802161512E-6	1.2320479292734915E-4	1.4494681520864605E-4
64-65	9.446205689169744	5.942819423554488E-4	3.6236703802161512E-6	1.2320479292734915E-4	1.485704855888622E-4
66-67	10.321496022170194	6.033411183059892E-4	3.6236703802161512E-6	1.2320479292734915E-4	1.558178263492945E-4
68-69	11.216939397990219	6.160239646367457E-4	3.6236703802161512E-6	1.2320479292734915E-4	1.5944149672951065E-4
70-71	12.131729549970215	6.268949757773942E-4	3.6236703802161512E-6	1.3045213368778144E-4	1.6668883748994297E-4
72-73	13.062664965329265	6.576961740092314E-4	3.6236703802161512E-6	1.3045213368778144E-4	1.7393617825037526E-4
74-75	14.008281681233761	6.667553499597719E-4	3.6236703802161512E-6	1.3226396887788952E-4	1.7393617825037526E-4
76-77	14.967726323309162	6.975565481916091E-4	3.6236703802161512E-6	1.340758040679976E-4	1.7574801344048333E-4
78-79	15.935568821490715	7.265459112333382E-4	3.6236703802161512E-6	1.4313498001853798E-4	1.8480718939102372E-4
80-81	16.913992437182493	7.42852427944311E-4	3.6236703802161512E-6	1.4494681520864605E-4	1.8843085977123986E-4
82-83	17.90397918505755	7.573471094651757E-4	3.6236703802161512E-6	1.503823207789703E-4	1.8843085977123986E-4
84-85	18.897678383237132	7.827128021266887E-4	3.6236703802161512E-6	1.630651671097268E-4	1.9024269496134794E-4
86-87	19.894896165355906	7.935838132673371E-4	3.6236703802161512E-6	1.6487700229983487E-4	1.9386636534156408E-4
88	20.645213354283463	8.008311540277694E-4	3.6236703802161512E-6	1.7031250787015911E-4	1.9930187091188832E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	96875	0.0	80.187614	8
GTGGTAT	98710	0.0	78.844536	6
TGGTATC	99150	0.0	78.32874	7
GTATCAA	99255	0.0	78.2222	9
AGTGGTA	100055	0.0	78.00073	5
AAGCAGT	109130	0.0	72.11905	1
GCAGTGG	110260	0.0	71.277435	3
CAGTGGT	110190	0.0	71.10792	4
AGCAGTG	115805	0.0	68.07015	2
CAACGCA	93405	0.0	43.112885	12-13
ATCAACG	95320	0.0	42.298508	10-11
TCAACGC	92920	0.0	41.643543	12-13
ACGCAGA	96900	0.0	41.555458	14-15
AACGCAG	95625	0.0	40.391827	14-15
AGAGTAC	98735	0.0	40.288113	18-19
GAGTACT	94235	0.0	39.369263	20-21
CGCAGAG	98640	0.0	39.00476	16-17
TATCAAC	100460	0.0	38.658348	10-11
AGTACTT	102025	0.0	38.09529	20-21
GTACTTT	99595	0.0	37.149036	22-23
>>END_MODULE