FastQCFastQC Report
Thu 26 May 2016
SRR1660602_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660602_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27567472
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7999842.9019128050624303No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC731520.26535621401918896Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA346240.12559729814906495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1092300.081.446438
AGTGGTA1113900.080.146785
GTATCAA1118850.079.430779
TGGTATC1124300.079.0875247
GTGGTAT1135550.078.548286
AAGCAGT1181550.076.7953641
GCAGTGG1200100.075.198633
CAGTGGT1205050.074.386044
AGCAGTG1275350.071.192822
CAACGCA1058750.043.8920712-13
ATCAACG1082500.042.94795210-11
ACGCAGA1093800.042.4807914-15
TCAACGC1054450.042.17672712-13
AGAGTAC1102050.041.47874518-19
AACGCAG1076750.041.22815314-15
GAGTACT1047700.040.4232220-21
CGCAGAG1110250.039.83676516-17
AGTACTT1121750.039.68231220-21
TATCAAC1142000.038.90067710-11
GTACTTT1106300.038.2378822-23
Produced by FastQC (version 0.11.5)