FastQCFastQC Report
Thu 26 May 2016
SRR1660602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27567472
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7347562.6653006122578087No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGAAATCTCGTATG428090.15528808735164398TruSeq Adapter, Index 13 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326800.11854550899698021No Hit
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACTGTGAAATCTCGTATG318190.11542226287560935TruSeq Adapter, Index 4 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1017500.080.7299968
GTATCAA1043600.078.4632959
AGTGGTA1050450.078.457185
TGGTATC1053300.077.9236457
GTGGTAT1066200.077.165976
AAGCAGT1105450.075.450211
GCAGTGG1121800.074.137873
CAGTGGT1138350.072.857194
AGCAGTG1189850.070.214642
CAACGCA982400.043.56281312-13
ATCAACG1003500.042.6281110-11
ACGCAGA1014450.042.1980914-15
TCAACGC978950.041.75770612-13
AGAGTAC1036350.040.805818-19
AACGCAG1000950.040.6990614-15
GAGTACT984850.039.75230420-21
CGCAGAG1026350.039.570516-17
AGTACTT1055350.039.05847520-21
TATCAAC1068450.038.26861610-11
GTACTTT1042750.037.4460422-23
Produced by FastQC (version 0.11.5)