FastQCFastQC Report
Thu 26 May 2016
SRR1660601_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660601_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27285475
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7036482.5788372751436435No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA452040.16567056281776293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1103950.078.094091
GTGGTAT1124100.075.320216
AGTGGTA1128550.075.119615
TGGTATC1142500.073.7825857
GCAGTGG1174700.072.87713
GGTATCA1162150.072.587858
CAGTGGT1195900.071.1441964
AGCAGTG1226550.070.155632
GTATCAA1202650.070.127799
CAACGCA1183950.037.23135812-13
ATCAACG1192500.037.13819510-11
GAGTACT1082050.036.48378820-21
ACGCAGA1227550.035.95868314-15
AGAGTAC1209650.035.85129518-19
TCAACGC1175800.035.70288512-13
AGTACTT1170400.035.6506720-21
AACGCAG1202400.034.82700314-15
GTACTTT1151100.034.3122222-23
TATCAAC1231300.034.25924710-11
CGCAGAG1238100.033.6841816-17
Produced by FastQC (version 0.11.5)