FastQCFastQC Report
Thu 26 May 2016
SRR1660601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27285475
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7335742.688514676764836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA387570.14204260691814968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1124000.074.728771
GTGGTAT1114650.074.402026
AGTGGTA1118650.074.274565
TGGTATC1129250.073.335957
GCAGTGG1158100.072.1747743
GGTATCA1151500.071.955638
CAGTGGT1184900.070.40324
GTATCAA1189950.069.531899
AGCAGTG1216300.069.027322
CAACGCA1172750.036.89409612-13
ATCAACG1180700.036.7848610-11
GAGTACT1077800.036.13904620-21
ACGCAGA1215450.035.6366214-15
AGAGTAC1208250.035.5107118-19
AGTACTT1169250.035.29913720-21
TCAACGC1164050.035.25102212-13
AACGCAG1190850.034.38669614-15
TATCAAC1221500.033.83144810-11
GTACTTT1153550.033.73536722-23
CGCAGAG1230850.033.21195616-17
Produced by FastQC (version 0.11.5)