FastQCFastQC Report
Thu 26 May 2016
SRR1660600_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660600_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37895444
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10996032.9016759903908236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA785440.2072650210933008No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC468820.1237140802466914Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379600.10017035293213611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1582800.082.349938
AGTGGTA1604600.081.667635
GTATCAA1612450.080.625879
TGGTATC1615550.080.5670857
GTGGTAT1629650.080.342596
AAGCAGT1675800.079.558011
GCAGTGG1736800.076.311363
CAGTGGT1747750.075.258064
AGCAGTG1867300.071.3476942
CAACGCA1530750.043.92073412-13
ATCAACG1561100.043.2479910-11
ACGCAGA1583600.042.55592714-15
TCAACGC1531450.042.32505412-13
AGAGTAC1594150.041.7651718-19
AACGCAG1562050.041.420814-15
GAGTACT1530450.040.60186820-21
CGCAGAG1604900.040.1962516-17
AGTACTT1628600.040.04602420-21
TATCAAC1621550.040.01977510-11
GTACTTT1630800.038.08699422-23
Produced by FastQC (version 0.11.5)