##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660600_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37895444 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07346128468636 34.0 31.0 34.0 30.0 34.0 2 31.81581968006497 34.0 31.0 34.0 30.0 34.0 3 32.283849372499766 34.0 31.0 34.0 30.0 34.0 4 35.82588429363699 37.0 35.0 37.0 35.0 37.0 5 35.860252277292226 37.0 35.0 37.0 35.0 37.0 6 35.918708961425544 37.0 36.0 37.0 35.0 37.0 7 35.86966277529299 37.0 35.0 37.0 35.0 37.0 8 35.90042433597031 37.0 36.0 37.0 35.0 37.0 9 37.64084394947319 39.0 38.0 39.0 35.0 39.0 10-11 37.53573959972603 39.0 38.0 39.0 35.0 39.0 12-13 37.55871472306803 39.0 38.0 39.0 35.0 39.0 14-15 39.00950598441332 41.0 39.0 41.0 36.0 41.0 16-17 38.93532329374476 41.0 39.0 41.0 36.0 41.0 18-19 38.763840581997144 40.0 38.5 41.0 35.0 41.0 20-21 38.71086255118162 40.0 38.5 41.0 35.0 41.0 22-23 38.569642567058985 40.0 38.0 41.0 34.5 41.0 24-25 38.5174615977583 40.0 38.0 41.0 34.0 41.0 26-27 38.474209129202976 40.0 38.0 41.0 34.0 41.0 28-29 38.364753689124214 40.0 38.0 41.0 34.0 41.0 30-31 38.24190095252611 40.0 38.0 41.0 34.0 41.0 32-33 38.34928155215704 40.0 38.0 41.0 34.0 41.0 34-35 38.184670167738375 40.0 38.0 41.0 33.5 41.0 36-37 38.099617291725096 40.0 38.0 41.0 33.0 41.0 38-39 37.81637394194405 40.0 37.0 41.0 33.0 41.0 40-41 37.77846252441323 40.0 37.0 41.0 33.0 41.0 42-43 37.80259021374707 40.0 37.0 41.0 33.0 41.0 44-45 37.762689797222066 40.0 37.0 41.0 33.0 41.0 46-47 37.61584857008141 40.0 37.0 41.0 33.0 41.0 48-49 37.50300400227531 40.0 37.0 41.0 32.5 41.0 50-51 36.28123223731063 38.5 34.5 40.0 30.0 40.5 52-53 36.355494502188705 38.5 35.0 39.5 30.5 40.5 54-55 36.70282742959813 39.5 36.0 41.0 30.0 41.0 56-57 36.522846242413735 39.0 35.0 41.0 29.5 41.0 58-59 36.25184448294101 39.0 35.0 41.0 28.5 41.0 60-61 36.05561960165977 39.0 35.0 41.0 28.5 41.0 62-63 35.78488547330386 38.5 35.0 41.0 28.0 41.0 64-65 35.48546864894894 37.5 35.0 40.0 28.0 41.0 66-67 35.14571784671529 37.0 34.5 40.0 27.5 41.0 68-69 34.80275675091707 36.5 34.0 39.5 27.0 41.0 70-71 34.446165692108 36.0 34.0 39.0 26.0 41.0 72-73 34.081967241233535 35.5 34.0 39.0 26.0 40.5 74-75 33.689418469407556 35.0 33.5 37.5 26.0 40.0 76-77 33.31974909701546 35.0 33.0 37.0 25.5 39.0 78-79 32.95278655397203 35.0 33.0 36.5 25.0 39.0 80-81 32.63611054405379 35.0 33.0 36.0 24.5 38.5 82-83 32.33216181607477 35.0 33.0 36.0 24.0 37.0 84-85 32.053135846620506 35.0 32.5 35.0 23.5 37.0 86-87 31.714549788623664 35.0 32.0 35.0 22.5 36.5 88-89 31.54233152671334 35.0 32.0 35.0 22.5 36.0 90-91 31.377231851406727 35.0 32.0 35.0 20.5 36.0 92-93 31.234924810486454 34.5 31.0 35.0 20.0 35.5 94-95 31.043051758411906 34.0 31.0 35.0 20.0 35.0 96-97 30.886816737125443 34.0 31.0 35.0 19.5 35.0 98-99 30.692597637858526 34.0 31.0 35.0 19.0 35.0 100 30.56825509156193 34.0 30.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5786675206483558 1101 2 0.9819181306613061 1101 3 0.5426249401580563 1101 4 0.32012920857317795 1101 5 0.2813269679909922 1101 6 0.23953147973102062 1101 7 0.2303963174359822 1101 8 0.24658022800984725 1101 9 0.2888899986811495 1101 10-11 0.31351970933766893 1101 12-13 0.30881035040282967 1101 14-15 0.3867808065238165 1101 16-17 0.4028212085993488 1101 18-19 0.43328451316919825 1101 20-21 0.3767077750682617 1101 22-23 0.3642945372527393 1101 24-25 0.37087694741756394 1101 26-27 0.38453071313092124 1101 28-29 0.4054634442095093 1101 30-31 0.3927190803523075 1101 32-33 0.3660874339394411 1101 34-35 0.4526515786895047 1101 36-37 0.4043944593895219 1101 38-39 0.40451716591785924 1101 40-41 0.39632870857946756 1101 42-43 0.39729442530832415 1101 44-45 0.3816157011792356 1101 46-47 0.40157441437687424 1101 48-49 0.39519346837170133 1101 50-51 0.3566841562369447 1101 52-53 0.429351352019232 1101 54-55 0.5733964791425592 1101 56-57 0.5055447559052624 1101 58-59 0.5330286033234657 1101 60-61 0.47572680056553196 1101 62-63 0.4515160862789429 1101 64-65 0.4049723730770438 1101 66-67 0.39757112854481846 1101 68-69 0.41191442237976617 1101 70-71 0.4259761133551976 1101 72-73 0.38699856364718954 1101 74-75 0.38196923639856095 1101 76-77 0.3786165624741855 1101 78-79 0.3998695518877966 1101 80-81 0.3722812134301492 1101 82-83 0.36346609044121436 1101 84-85 0.36562610894145564 1101 86-87 0.4976260887831998 1101 88-89 0.420676103316282 1101 90-91 0.42490532091774824 1101 92-93 0.385702795164228 1101 94-95 0.3762850954672068 1101 96-97 0.33305744553907246 1101 98-99 0.3619941038857881 1101 100 0.37553466592564533 1102 1 1.5799936304005051 1102 2 0.9862312794886847 1102 3 0.5254643501520277 1102 4 0.3235761994154558 1102 5 0.27828279468125316 1102 6 0.25075595233942494 1102 7 0.22703852875660147 1102 8 0.2304837849236705 1102 9 0.2635454466060736 1102 10-11 0.2958085077685837 1102 12-13 0.3165039372855887 1102 14-15 0.393853977413535 1102 16-17 0.3946380874724724 1102 18-19 0.4097297850509136 1102 20-21 0.3641410217010801 1102 22-23 0.3596879805848019 1102 24-25 0.39225005350365194 1102 26-27 0.3592792004067533 1102 28-29 0.39803885292734265 1102 30-31 0.3969254918150753 1102 32-33 0.34967110267789536 1102 34-35 0.4692291902024337 1102 36-37 0.39301400758006366 1102 38-39 0.3924012634545946 1102 40-41 0.3623271987207417 1102 42-43 0.4028483189129588 1102 44-45 0.3998140387499163 1102 46-47 0.431044585173602 1102 48-49 0.4010928272523344 1102 50-51 0.36925612344563064 1102 52-53 0.42203669991115333 1102 54-55 0.5834283400123041 1102 56-57 0.5078544814626014 1102 58-59 0.5473841902044327 1102 60-61 0.46556576474802824 1102 62-63 0.4371096068231992 1102 64-65 0.40608931247808044 1102 66-67 0.3763127082842246 1102 68-69 0.3644196651247711 1102 70-71 0.37011484105726566 1102 72-73 0.3984391023325742 1102 74-75 0.39351350374433025 1102 76-77 0.3888392237803444 1102 78-79 0.3910552257911988 1102 80-81 0.38812846354674235 1102 82-83 0.3579773854283914 1102 84-85 0.3981190557413825 1102 86-87 0.4934336867942051 1102 88-89 0.4180326669858445 1102 90-91 0.43887521434059806 1102 92-93 0.36713847332840643 1102 94-95 0.3093325246558898 1102 96-97 0.32572360126173194 1102 98-99 0.35206112535779255 1102 100 0.3807104594685846 1103 1 1.5272961764479085 1103 2 0.907430507093153 1103 3 0.4569488924811296 1103 4 0.2907420809590917 1103 5 0.24859626608068197 1103 6 0.22536067744486132 1103 7 0.1987221081680275 1103 8 0.2055051991445893 1103 9 0.2439131309264866 1103 10-11 0.25543408880257346 1103 12-13 0.2779571957218536 1103 14-15 0.32488238902882927 1103 16-17 0.3269788433612888 1103 18-19 0.350043100284644 1103 20-21 0.2943952711648521 1103 22-23 0.28307814066244674 1103 24-25 0.3062727978658799 1103 26-27 0.27123835565966914 1103 28-29 0.29254202738550816 1103 30-31 0.29651885391413657 1103 32-33 0.29356862948177564 1103 34-35 0.3803096049941317 1103 36-37 0.3041029002169324 1103 38-39 0.2803685190220975 1103 40-41 0.279136946349162 1103 42-43 0.30202178269150437 1103 44-45 0.2746276452326697 1103 46-47 0.3270143233852423 1103 48-49 0.2781490551012027 1103 50-51 0.22411903924655263 1103 52-53 0.32397903416664775 1103 54-55 0.4728542085473393 1103 56-57 0.40524020135912764 1103 58-59 0.45961688138442724 1103 60-61 0.38821902576102474 1103 62-63 0.3683188680735725 1103 64-65 0.362775322218134 1103 66-67 0.3728867647896621 1103 68-69 0.35702349383910814 1103 70-71 0.37719945202119476 1103 72-73 0.3769862128377568 1103 74-75 0.35835123392280366 1103 76-77 0.35633743177899646 1103 78-79 0.373210259675254 1103 80-81 0.33366175768248496 1103 82-83 0.3611659453542373 1103 84-85 0.3642008935929866 1103 86-87 0.4549991737071224 1103 88-89 0.39673595489838576 1103 90-91 0.38838429771824323 1103 92-93 0.31641783696881376 1103 94-95 0.3334888121600592 1103 96-97 0.33891592087669054 1103 98-99 0.3480173474285344 1103 100 0.3360307240757372 1104 1 1.4678247152878505 1104 2 0.8580109183055455 1104 3 0.41203237408394244 1104 4 0.24437805594993733 1104 5 0.2207337256429298 1104 6 0.1672604875352235 1104 7 0.17448353194002664 1104 8 0.16923554598528767 1104 9 0.2177368907159476 1104 10-11 0.2117456539307554 1104 12-13 0.234004673494141 1104 14-15 0.2794121392986497 1104 16-17 0.28650796026603587 1104 18-19 0.32070051779327 1104 20-21 0.27955160745800356 1104 22-23 0.2515273791987198 1104 24-25 0.27311887271864066 1104 26-27 0.24527358900952123 1104 28-29 0.2889924234134753 1104 30-31 0.26305619439466454 1104 32-33 0.2400791143751846 1104 34-35 0.3513437839159934 1104 36-37 0.28427480321609266 1104 38-39 0.30479585393265296 1104 40-41 0.24000292984117522 1104 42-43 0.24842055742292501 1104 44-45 0.226946608155707 1104 46-47 0.29932484371076384 1104 48-49 0.29466956305363823 1104 50-51 0.18605073325249322 1104 52-53 0.24432339861589725 1104 54-55 0.4195976469305833 1104 56-57 0.3631973333949077 1104 58-59 0.38266318077986483 1104 60-61 0.28636459048929197 1104 62-63 0.30471102448051113 1104 64-65 0.3239854594917233 1104 66-67 0.27838730662326583 1104 68-69 0.29089628561972347 1104 70-71 0.2623944925956039 1104 72-73 0.28066602622616443 1104 74-75 0.2904017326875774 1104 76-77 0.289909868221379 1104 78-79 0.3289487628960117 1104 80-81 0.2643790483945949 1104 82-83 0.24651683686892767 1104 84-85 0.27564031220107665 1104 86-87 0.3492076616889932 1104 88-89 0.2748148805405073 1104 90-91 0.29704155150540146 1104 92-93 0.26439277814077755 1104 94-95 0.25512567631373884 1104 96-97 0.24602670736991072 1104 98-99 0.2708171851781067 1104 100 0.2976127701418534 1105 1 1.4191408162881949 1105 2 0.8232150972150976 1105 3 0.36761830061536216 1105 4 0.18652095189316498 1105 5 0.17977876957622385 1105 6 0.13700632989718287 1105 7 0.1348849804634753 1105 8 0.14235062844331736 1105 9 0.16436331522117342 1105 10-11 0.18289185716142242 1105 12-13 0.18535023725964805 1105 14-15 0.20359054992396608 1105 16-17 0.21919387956234004 1105 18-19 0.2664091879566328 1105 20-21 0.22858799727013235 1105 22-23 0.22864999366176875 1105 24-25 0.25478725197290686 1105 26-27 0.239365229527003 1105 28-29 0.22793044094243697 1105 30-31 0.22956045749722165 1105 32-33 0.20480952220139415 1105 34-35 0.30573668008566557 1105 36-37 0.2609889603105131 1105 38-39 0.2751255982714511 1105 40-41 0.20580705885576123 1105 42-43 0.1966264701448992 1105 44-45 0.2293120504459054 1105 46-47 0.2610240091632363 1105 48-49 0.23848514072060567 1105 50-51 0.1189569151822738 1105 52-53 0.2350584607040318 1105 54-55 0.3953780633501367 1105 56-57 0.33642944156677146 1105 58-59 0.39225568572329195 1105 60-61 0.3386939244910465 1105 62-63 0.30103100871647115 1105 64-65 0.25292658684284675 1105 66-67 0.27835946619367036 1105 68-69 0.27478523001613553 1105 70-71 0.28200475841001804 1105 72-73 0.28182900973115466 1105 74-75 0.3050616965279076 1105 76-77 0.2680252369266185 1105 78-79 0.28229269404932467 1105 80-81 0.23829372092529155 1105 82-83 0.22714097039761327 1105 84-85 0.2534616885601011 1105 86-87 0.38729523466032845 1105 88-89 0.33989991576931544 1105 90-91 0.3073203577241017 1105 92-93 0.24123264317431037 1105 94-95 0.2684528040830223 1105 96-97 0.22587611111863382 1105 98-99 0.30356444213372313 1105 100 0.32487143943035335 1106 1 1.401864033024541 1106 2 0.79629765450386 1106 3 0.3624274326497172 1106 4 0.21353208820046632 1106 5 0.17285236497892242 1106 6 0.14224756159269702 1106 7 0.12799240803838074 1106 8 0.09395790427191031 1106 9 0.11096914685276005 1106 10-11 0.16232388240131002 1106 12-13 0.17810349832579675 1106 14-15 0.20310676252083937 1106 16-17 0.19969936497117402 1106 18-19 0.248734068052201 1106 20-21 0.1901744251698645 1106 22-23 0.18459033285730442 1106 24-25 0.18459876958682742 1106 26-27 0.1648233567782924 1106 28-29 0.17077174873344347 1106 30-31 0.13598222233175505 1106 32-33 0.12617074034441345 1106 34-35 0.2744527097482816 1106 36-37 0.19764800672082572 1106 38-39 0.19955367671467883 1106 40-41 0.14532173365540046 1106 42-43 0.14166954816870714 1106 44-45 0.12875734682214102 1106 46-47 0.17180309113943082 1106 48-49 0.1693718995189144 1106 50-51 0.07112839786709202 1106 52-53 0.15539929942573139 1106 54-55 0.272506563114689 1106 56-57 0.23706262775969833 1106 58-59 0.30038463098696155 1106 60-61 0.21395511938354872 1106 62-63 0.2032612680751953 1106 64-65 0.20179412687855347 1106 66-67 0.1659311401964132 1106 68-69 0.21136694586015636 1106 70-71 0.19056608136632036 1106 72-73 0.1317903519645114 1106 74-75 0.19428685069414087 1106 76-77 0.18630689188064764 1106 78-79 0.20676349787373027 1106 80-81 0.19091094485251858 1106 82-83 0.1489711771376605 1106 84-85 0.17111445676049186 1106 86-87 0.3636642551700504 1106 88-89 0.2841835406482538 1106 90-91 0.26612668858752286 1106 92-93 0.21797905697592412 1106 94-95 0.1721356662696003 1106 96-97 0.21200280244438474 1106 98-99 0.24108895522776308 1106 100 0.2600129356861771 1107 1 1.3804208233810193 1107 2 0.7723170318878303 1107 3 0.3138727696131909 1107 4 0.19817099682481398 1107 5 0.13522013676042377 1107 6 0.09594133339540889 1107 7 0.1106893080068545 1107 8 0.10714777496261974 1107 9 0.09773525368644442 1107 10-11 0.11627328847811214 1107 12-13 0.13192537789317527 1107 14-15 0.17890076430881408 1107 16-17 0.18395764420456828 1107 18-19 0.2104781774556841 1107 20-21 0.15609970693597575 1107 22-23 0.15639224542965025 1107 24-25 0.17490435338807941 1107 26-27 0.14319190357866063 1107 28-29 0.1790139295156976 1107 30-31 0.1606029539540259 1107 32-33 0.15171524392417268 1107 34-35 0.2523554803169148 1107 36-37 0.18065752698983317 1107 38-39 0.20374394056256762 1107 40-41 0.11159118756314967 1107 42-43 0.1575146640728775 1107 44-45 0.12428927711607685 1107 46-47 0.1733063671503956 1107 48-49 0.16107467765113626 1107 50-51 0.03510648422078333 1107 52-53 0.11284712640055972 1107 54-55 0.24887767786656667 1107 56-57 0.2264002590086136 1107 58-59 0.27606210186277025 1107 60-61 0.18944765904155503 1107 62-63 0.2077920725185578 1107 64-65 0.16769029317612194 1107 66-67 0.157280204099024 1107 68-69 0.17533439219930358 1107 70-71 0.1537510764910195 1107 72-73 0.1635214201086015 1107 74-75 0.17006358036751124 1107 76-77 0.15139086421466885 1107 78-79 0.20212090758015933 1107 80-81 0.17054105456417545 1107 82-83 0.13331932748815944 1107 84-85 0.20106288114396165 1107 86-87 0.3064576872774012 1107 88-89 0.22830917488160907 1107 90-91 0.242435612740163 1107 92-93 0.19069144093080226 1107 94-95 0.18065594168178123 1107 96-97 0.15793220902930827 1107 98-99 0.22162461458215432 1107 100 0.24823060240345285 1108 1 1.3144463791151004 1108 2 0.6982728883594049 1108 3 0.2586751741530904 1108 4 0.11416595188192957 1108 5 0.09601434498279104 1108 6 0.06457281839200846 1108 7 0.04294363060156314 1108 8 0.0832844290565049 1108 9 0.07881698092599976 1108 10-11 0.08389310329616251 1108 12-13 0.08240677109859007 1108 14-15 0.11013823765654962 1108 16-17 0.0948428136982784 1108 18-19 0.15486263027815994 1108 20-21 0.09747909706033653 1108 22-23 0.07651764975782527 1108 24-25 0.09524743903740784 1108 26-27 0.07781759221641238 1108 28-29 0.09107045196657992 1108 30-31 0.04109653665235413 1108 32-33 0.019030884981319218 1108 34-35 0.135386885736132 1108 36-37 0.08903641574235621 1108 38-39 0.06475047227338848 1108 40-41 0.030206101193449797 1108 42-43 0.026100242181520628 1108 44-45 0.0158630136252782 1108 46-47 0.07027473316630051 1108 48-49 0.06384631069364133 1108 50-51 -0.07134231715451023 1108 52-53 0.002732781328823819 1108 54-55 0.18572545197690715 1108 56-57 0.12151471202753328 1108 58-59 0.1521970381393274 1108 60-61 0.0743181185375974 1108 62-63 0.09443329827345082 1108 64-65 0.06780827787761723 1108 66-67 0.054045534243492455 1108 68-69 0.052803779050151434 1108 70-71 0.08144112071647669 1108 72-73 0.0907443145653204 1108 74-75 0.11359315360984112 1108 76-77 0.14030176874661748 1108 78-79 0.16629307073891653 1108 80-81 0.10717701228166021 1108 82-83 0.10053824550007562 1108 84-85 0.08755108095503061 1108 86-87 0.20850790315028433 1108 88-89 0.13255385659464025 1108 90-91 0.13779450250169845 1108 92-93 0.08498674381307936 1108 94-95 0.11465047258552019 1108 96-97 0.09672267642865862 1108 98-99 0.14271049818766457 1108 100 0.170798344122705 1109 1 1.2369345544857033 1109 2 0.634785847606949 1109 3 0.19992290982273886 1109 4 0.11458008965706767 1109 5 0.09005509612106266 1109 6 0.043511781935151816 1109 7 -0.011706165575127159 1109 8 0.01418303030079926 1109 9 0.010593999740272864 1109 10-11 -0.029737600629573535 1109 12-13 0.03306858206523344 1109 14-15 0.02269167165924557 1109 16-17 0.035049224211476826 1109 18-19 0.0848175778893534 1109 20-21 0.023968994242864028 1109 22-23 0.015663371760155087 1109 24-25 0.024910216351777592 1109 26-27 0.016422194128288936 1109 28-29 0.015366154115874053 1109 30-31 -0.002048591586159887 1109 32-33 -0.024366052911325653 1109 34-35 0.04333563569462484 1109 36-37 0.014362011861472013 1109 38-39 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-0.6147436298572799 2316 52-53 -0.6048097076555408 2316 54-55 -0.5797674982242214 2316 56-57 -0.5775659710250807 2316 58-59 -0.5169210153144448 2316 60-61 -0.5544372544589251 2316 62-63 -0.562869680535826 2316 64-65 -0.5955573026844974 2316 66-67 -0.6331322939044597 2316 68-69 -0.6222935578484794 2316 70-71 -0.634142386783985 2316 72-73 -0.8554510904357002 2316 74-75 -0.7305322593900172 2316 76-77 -0.6734044189132007 2316 78-79 -0.6516901317195263 2316 80-81 -0.893172567941015 2316 82-83 -0.7312103041835556 2316 84-85 -0.6639393430464224 2316 86-87 -0.5550995494612145 2316 88-89 -0.7655067178982193 2316 90-91 -0.8158780821997063 2316 92-93 -0.8490439920539323 2316 94-95 -0.7749003601281785 2316 96-97 -0.8288793461403863 2316 98-99 -0.9397043126231353 2316 100 -0.8962299363651844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 27.0 4 47.0 5 287.0 6 7155.0 7 7914.0 8 19907.0 9 29032.0 10 32868.0 11 32602.0 12 34909.0 13 39182.0 14 45865.0 15 59234.0 16 76585.0 17 95697.0 18 104814.0 19 115657.0 20 130777.0 21 158922.0 22 206119.0 23 291914.0 24 401288.0 25 481770.0 26 486171.0 27 453073.0 28 425424.0 29 464771.0 30 555108.0 31 688761.0 32 877974.0 33 1169153.0 34 1677691.0 35 2673068.0 36 4731155.0 37 8913017.0 38 9927734.0 39 2476158.0 40 3614.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.57237653244918 32.585385116323465 27.34997644395444 21.492261907272916 2 22.254829648452272 30.90021591886456 26.615090813617208 20.229863619065956 3 24.87650537096839 26.113550907025896 26.612828336349992 22.39711538565572 4 21.4899028416021 26.834717779369317 26.079886380249334 25.59549299877925 5 22.134422491565036 29.796540452233838 26.110651199382673 21.958385856818452 6 25.82430957855854 26.246373541344987 26.17316156843247 21.756155311664006 7 22.04364989100009 26.358825615026436 29.759862425678403 21.837662068295067 8 25.410627659826286 26.584692473540837 26.164173692443644 21.840506174189237 9 25.222713001382157 26.3215287286307 26.49209180792932 21.963666462057823 10-11 21.668887403559708 28.542628186630775 28.094131958400194 21.694352451409323 12-13 21.72574137718698 26.66223762359673 27.982923167898388 23.6290978313179 14-15 21.684194062689816 30.353655338431835 26.232548580772804 21.729602018105552 16-17 23.60884332164046 26.596625969074278 26.17886730658176 23.6156634027035 18-19 21.8582262693104 28.41959172404659 26.187313744034984 23.53486826260803 20-21 23.579407194376955 28.47520303866705 26.194980352446628 21.750409414509363 22-23 23.642222439524264 26.689154084209516 27.945849674106775 21.72277380215944 24-25 21.877049625231752 28.3880281980912 26.055890623576055 23.679031553100995 26-27 21.80929243925141 26.696736380249174 29.692065879547307 21.801905300952107 28-29 21.951403604351235 26.668092020867185 29.657719665559114 21.722784709222466 30-31 21.949392700394878 26.571539789881587 29.679630428942648 21.79943708078088 32-33 21.952506612324417 26.60823655265275 29.640601387619412 21.798655447403423 34-35 21.977769538877254 26.773039283771016 29.51176938905667 21.737421788295062 36-37 21.915893886920276 26.731777410925783 29.573301309480716 21.779027392673225 38-39 21.980246056613222 26.658310710261706 29.531943307302434 21.829499925822642 40-41 22.04292857639579 26.748330376527303 29.443899045101162 21.764842001975737 42-43 22.094668556762727 26.765694294537468 29.431589468643494 21.70804768005631 44-45 22.22621628810605 26.789306974346587 29.31344000749795 21.671036730049416 46-47 22.150685979934053 26.89622259124907 29.295695710251675 21.657395718565205 48-49 22.309647683922368 26.88375495043454 29.109077806123594 21.6975195595195 50-51 22.392640956125753 26.900803223024173 29.031646195435506 21.67490962541457 52-53 22.45878452183382 27.091818637429032 28.888534810224158 21.560862030512986 54-55 22.801081697440786 27.16785914831267 28.33156315643364 21.69949599781291 56-57 23.126833793978193 27.542123215751257 27.67644570459975 21.6545972856708 58-59 23.353566513787197 27.814259561807695 27.298935078863078 21.533238845542023 60-61 23.516444782163013 28.00289878036341 26.98524268667491 21.49541375079867 62-63 23.680137114742074 28.063372122004065 26.872683498744987 21.38380726450887 64-65 23.836683539614338 28.13587945975156 26.817990657551356 21.209446343082742 66-67 24.026782527374348 27.999805200705634 26.852459793115496 21.120952478804526 68-69 24.159608373792285 27.876390998520396 26.961726778901628 21.00227384878569 70-71 24.341080121223268 27.808891106245014 27.027909190793775 20.822119581737937 72-73 24.54093816712459 27.708189528712573 27.001453128658632 20.749419175504205 74-75 24.756756789575956 27.72834735001758 26.89004021650997 20.624855643896495 76-77 24.98846507462191 27.789010979031403 26.694258617363094 20.528265328983597 78-79 25.24829047347853 27.83093087044815 26.437972335043348 20.482806321029972 80-81 25.47937669150622 27.866566491835894 26.234099973495557 20.41995684316233 82-83 25.64645191670524 27.918814753577593 26.085456806576563 20.34927652314061 84-85 25.822659345933165 27.877247893324437 25.957538950313076 20.34255381042933 86-87 25.980909722758106 27.804223240042685 25.881835417979758 20.333031619219447 88-89 26.124252918969702 27.812637702502357 25.824682750063786 20.23842662846415 90-91 26.27405087213446 27.74218478629248 25.758443144181705 20.22532119739136 92-93 26.377262599861712 27.72852561652973 25.719159471329338 20.17505231227922 94-95 26.478585427546502 27.7719463405359 25.657153221544743 20.092315010372857 96-97 26.54202688174072 27.790328292735296 25.572167236842137 20.09547758868185 98-99 26.59981432446684 27.84102669107235 25.521664906011083 20.03749407844973 100 26.609895574177056 27.98150979741225 25.434927226904346 19.97366740150635 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 89642.0 1 53489.0 2 13259.5 3 8553.5 4 7994.5 5 8315.5 6 8760.0 7 9050.0 8 9320.5 9 9566.0 10 10014.0 11 13210.5 12 92114.0 13 110336.5 14 50816.5 15 52205.5 16 59161.0 17 66857.5 18 74357.0 19 82318.5 20 92735.0 21 109267.5 22 132552.5 23 159936.0 24 190481.0 25 224939.0 26 261931.0 27 307066.0 28 365093.0 29 425407.0 30 480519.0 31 535600.0 32 590940.5 33 639014.0 34 685005.5 35 741564.0 36 807060.0 37 867097.5 38 937525.5 39 1002868.5 40 1043248.5 41 1099709.0 42 1170725.5 43 1208075.5 44 1228261.0 45 1272822.0 46 1325786.0 47 1367433.0 48 1393831.0 49 1406383.5 50 1410090.0 51 1411164.0 52 1405287.5 53 1371507.0 54 1311504.5 55 1241225.0 56 1159584.0 57 1061084.5 58 944376.5 59 810395.0 60 667149.5 61 536497.5 62 422620.0 63 320047.5 64 236608.0 65 173765.5 66 127417.5 67 95260.5 68 71943.0 69 55018.5 70 42830.0 71 34203.0 72 28223.5 73 22765.0 74 18579.0 75 15818.5 76 12332.5 77 8269.0 78 5585.0 79 4029.5 80 2941.0 81 2212.5 82 1653.0 83 1169.5 84 851.0 85 676.0 86 572.5 87 494.5 88 419.5 89 354.5 90 284.5 91 252.5 92 224.5 93 179.0 94 147.5 95 115.0 96 96.5 97 76.5 98 57.5 99 47.5 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.292397260208905 2 5.040183722349314E-4 3 0.0023248177274291866 4 0.0016466359386104567 5 0.001295670265797651 6 7.916518935627197E-6 7 0.0 8 9.156773568875457E-4 9 9.842871876629814E-4 10-11 9.631764705013088E-4 12-13 3.2985495565113313E-4 14-15 2.6916164381132464E-4 16-17 0.0 18-19 0.061133998060558414 20-21 0.050400517803670546 22-23 0.04427972924660811 24-25 0.043720295241823796 26-27 0.03175183803097808 28-29 0.026715612568096573 30-31 0.02612847074703756 32-33 0.026726167926677414 34-35 0.02673144560596783 36-37 0.027311990327913822 38-39 0.026109998869521096 40-41 0.03002603690301135 42-43 0.02982548508997546 44-45 0.04860478742510577 46-47 0.04602400225209131 48-49 0.043398356805108285 50-51 0.041536655435413294 52-53 0.04282308976245271 54-55 0.029774027716893886 56-57 0.02868946462271296 58-59 0.027652400642145792 60-61 0.02600708412335794 62-63 0.025096684445760815 64-65 0.02859578581530804 66-67 0.02717081240689514 68-69 0.027282963091816523 70-71 0.025025435775340173 72-73 0.031699061238073895 74-75 0.03658091458171067 76-77 0.042006368892260504 78-79 0.038422824654066594 80-81 0.03959315003671681 82-83 0.026359369215993356 84-85 0.025873822721274885 86-87 0.027028315066053854 88-89 0.02725921353500964 90-91 0.023971219337079148 92-93 0.025703617564158898 94-95 0.027107480255410123 96-97 0.027240741657493177 98-99 0.024967381303145568 100 0.026607420142643005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.7895444E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.77127338723235 #Duplication Level Percentage of deduplicated Percentage of total 1 73.64782348772401 32.236590162558 2 12.767353804775883 11.176866676407323 3 4.668242722880722 6.1300478538351015 4 2.398703580612997 4.199772408077785 5 1.518406643998976 3.3231296163734583 6 0.9722563469420471 2.5534139018683297 7 0.7345850092472509 2.2507604886146813 8 0.520725985574481 1.823427157953329 9 0.4046305435518295 1.5940074728368399 >10 2.076445717934611 16.22860719343172 >50 0.16167679991538472 4.9740310992839865 >100 0.12751314030829144 9.423265068532501 >500 0.0011767960722745736 0.31936988611784733 >1k 4.2319210708087815E-4 0.36577944649354094 >5k 6.038061435499591E-6 0.01350276195633401 >10k+ 3.019029285682644E-5 3.387428805659245 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1099603 2.9016759903908236 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 78544 0.2072650210933008 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 46882 0.1237140802466914 Illumina Single End PCR Primer 1 (100% over 50bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37960 0.10017035293213611 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.1392304573605207 2.6388396452090653E-6 0.0 0.0 2.6388396452090653E-6 2 0.13923573503981113 2.3749556806881587E-5 0.0 0.0 2.6388396452090653E-6 3 0.13924101271910153 3.430491538771785E-5 0.0 0.0 2.6388396452090653E-6 4 0.13924365155874674 4.486027396855411E-5 0.0 7.916518935627195E-6 1.0555358580836261E-5 5 0.13924629039839195 5.805447219459944E-5 0.0 7.916518935627195E-6 1.0555358580836261E-5 6 0.13933601094632905 6.333215148501756E-5 0.0 7.916518935627195E-6 1.3194198226045326E-5 7 0.13933601094632905 6.86098307754357E-5 0.0 1.0555358580836261E-5 1.3194198226045326E-5 8 0.13933864978597427 6.86098307754357E-5 0.0 1.0555358580836261E-5 1.3194198226045326E-5 9 0.13934128862561948 7.916518935627196E-5 0.0 1.0555358580836261E-5 1.3194198226045326E-5 10-11 0.13934920514455512 9.631764705013088E-5 0.0 1.0555358580836261E-5 1.8471877516463458E-5 12-13 0.1393676770220716 1.1215068492138528E-4 0.0 1.0555358580836261E-5 2.638839645209065E-5 14-15 0.13940198193745929 1.3062256243784873E-4 0.0 1.583303787125439E-5 3.562433521032239E-5 16-17 0.13943232859337917 1.4645560030910313E-4 0.0 2.1110717161672522E-5 3.6943755032926916E-5 18-19 0.13947586944752516 1.5964979853514845E-4 0.0 2.1110717161672522E-5 4.0902014500740516E-5 20-21 0.13955635405670402 1.7284399676119376E-4 0.0 2.1110717161672522E-5 5.013795325897224E-5 22-23 0.13991787508809767 1.860381949872391E-4 0.0 2.638839645209065E-5 5.541563254939037E-5 24-25 0.14131646010005847 2.0055181303588895E-4 0.0 2.7707816274695187E-5 5.67350523719949E-5 26-27 0.1463065058691488 2.2562078966537508E-4 0.0 3.166607574250878E-5 6.333215148501756E-5 28-29 0.1613465724270179 2.4145382753662948E-4 1.3194198226045326E-6 3.166607574250878E-5 6.729041095283116E-5 30-31 0.19394415856428548 2.506897662948612E-4 2.6388396452090653E-6 3.2985495565113316E-5 7.25680902432493E-5 32-33 0.2538339437321278 2.6124512487569747E-4 2.6388396452090653E-6 3.430491538771785E-5 7.784576953366742E-5 34-35 0.34970826572186353 2.718004834565337E-4 2.6388396452090653E-6 3.6943755032926916E-5 8.312344882408556E-5 36-37 0.48981745668423887 2.995082997312289E-4 2.6388396452090653E-6 3.958259467813598E-5 9.103996775971276E-5 38-39 0.6833552339431621 3.206190168929014E-4 2.6388396452090653E-6 3.958259467813598E-5 9.235938758231729E-5 40-41 0.9286261430265865 3.4172973405457394E-4 2.6388396452090653E-6 3.958259467813598E-5 9.631764705013088E-5 42-43 1.2227129467067335 3.5756277192582834E-4 2.6388396452090653E-6 4.2221434323345044E-5 1.0027590651794448E-4 44-45 1.565164403404272 3.720763899744782E-4 2.6388396452090653E-6 4.354085414594957E-5 1.0291474616315355E-4 46-47 1.9610220162613743 3.852705882005235E-4 2.6388396452090653E-6 4.88185334363677E-5 1.1083126509878075E-4 48-49 2.412255679073189 4.0110362607177796E-4 2.6388396452090653E-6 5.541563254939037E-5 1.174283642118034E-4 50-51 2.913197164281806 4.1561724412042777E-4 3.958259467813598E-6 6.06933118398085E-5 1.2270604350222153E-4 52-53 3.456232100090977 4.367279612821003E-4 5.2776792904181305E-6 6.597099113022663E-5 1.332614020830578E-4 54-55 4.043468919377221 4.4596390004033204E-4 5.2776792904181305E-6 7.25680902432493E-5 1.3458082190566232E-4 56-57 4.668017611826899 4.525609991533547E-4 5.2776792904181305E-6 8.444286864669009E-5 1.41177921018685E-4 58-59 5.334729684127728 4.617969379115864E-4 5.2776792904181305E-6 8.708170829189915E-5 1.4645560030910313E-4 60-61 6.041420177053474 4.815882352506544E-4 5.2776792904181305E-6 8.708170829189915E-5 1.5833037871254392E-4 62-63 6.774464761515922 4.987406929445133E-4 5.2776792904181305E-6 8.708170829189915E-5 1.622886381803575E-4 64-65 7.535098414469032 5.172125704609768E-4 5.2776792904181305E-6 8.972054793710822E-5 1.6624689764817112E-4 66-67 8.320514729950123 5.383232876226493E-4 5.2776792904181305E-6 9.235938758231729E-5 1.7152457693858924E-4 68-69 9.134229961786435 5.462398065582766E-4 5.2776792904181305E-6 9.367880740492182E-5 1.781216760516119E-4 70-71 9.966943519648432 5.581145849617173E-4 5.2776792904181305E-6 9.499822722752635E-5 1.8339935534203005E-4 72-73 10.817275290401664 5.67350523719949E-4 5.2776792904181305E-6 9.499822722752635E-5 1.9395471392286628E-4 74-75 11.681124517237482 5.831835615912034E-4 5.2776792904181305E-6 1.0027590651794449E-4 1.9659355356807536E-4 76-77 12.557970029325954 5.897806607042262E-4 5.2776792904181305E-6 1.055535858083626E-4 2.1242659143932974E-4 78-79 13.44851639685235 5.976971796398532E-4 5.2776792904181305E-6 1.055535858083626E-4 2.2430136984277055E-4 80-81 14.351648710066572 6.082525382206895E-4 5.2776792904181305E-6 1.0687300563096713E-4 2.269402094879796E-4 82-83 15.26782348822724 6.320020950275711E-4 5.2776792904181305E-6 1.1083126509878075E-4 2.2957904913318867E-4 84-85 16.195217293139514 6.451962932536165E-4 5.2776792904181305E-6 1.1478952456659433E-4 2.3221788877839772E-4 86-87 17.12792176283777 6.610293311248708E-4 5.2776792904181305E-6 1.1874778403440793E-4 2.3881498789142042E-4 88 17.837070334893028 6.834594681091479E-4 5.2776792904181305E-6 1.2402546332482606E-4 2.4541208700444307E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 158280 0.0 82.34993 8 AGTGGTA 160460 0.0 81.66763 5 GTATCAA 161245 0.0 80.62587 9 TGGTATC 161555 0.0 80.567085 7 GTGGTAT 162965 0.0 80.34259 6 AAGCAGT 167580 0.0 79.55801 1 GCAGTGG 173680 0.0 76.31136 3 CAGTGGT 174775 0.0 75.25806 4 AGCAGTG 186730 0.0 71.347694 2 CAACGCA 153075 0.0 43.920734 12-13 ATCAACG 156110 0.0 43.24799 10-11 ACGCAGA 158360 0.0 42.555927 14-15 TCAACGC 153145 0.0 42.325054 12-13 AGAGTAC 159415 0.0 41.76517 18-19 AACGCAG 156205 0.0 41.4208 14-15 GAGTACT 153045 0.0 40.601868 20-21 CGCAGAG 160490 0.0 40.19625 16-17 AGTACTT 162860 0.0 40.046024 20-21 TATCAAC 162155 0.0 40.019775 10-11 GTACTTT 163080 0.0 38.086994 22-23 >>END_MODULE