FastQCFastQC Report
Thu 26 May 2016
SRR1660600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37895444
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11317842.9865964890132966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA712460.18800676936256505No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473210.12487253085093816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1532650.082.16348
AGTGGTA1560500.081.043325
GTATCAA1557600.080.578899
TGGTATC1561450.080.482497
GTGGTAT1584150.079.747426
AAGCAGT1679400.076.100391
GCAGTGG1685000.075.635613
CAGTGGT1702550.074.6073464
AGCAGTG1819350.070.394432
CAACGCA1474450.043.9539712-13
ATCAACG1498100.043.37901310-11
ACGCAGA1526300.042.5839214-15
TCAACGC1473050.042.30881512-13
AGAGTAC1558500.041.47039818-19
AACGCAG1503800.041.3765214-15
GAGTACT1487100.040.5885620-21
TATCAAC1566750.039.94321410-11
AGTACTT1586150.039.93004620-21
CGCAGAG1557200.039.9229316-17
GTACTTT1592500.037.8579522-23
Produced by FastQC (version 0.11.5)