FastQCFastQC Report
Thu 26 May 2016
SRR1660599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31466306
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10913533.468322592426324No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA496970.15793719161060724No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC473520.15048477568355179Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1568100.081.979918
AGTGGTA1584500.081.4274145
GTGGTAT1588850.081.118756
AAGCAGT1621000.081.028131
TGGTATC1591800.080.582047
GTATCAA1603950.080.001179
GCAGTGG1672950.077.9744643
CAGTGGT1712450.075.7053764
AGCAGTG1761100.074.426282
CAACGCA1524200.043.5672212-13
ATCAACG1541650.043.24606710-11
ACGCAGA1563900.042.51795214-15
TCAACGC1526600.041.9364612-13
AGAGTAC1564350.041.60410718-19
AACGCAG1549350.041.22178314-15
GAGTACT1475500.041.13744420-21
CGCAGAG1574500.040.3551416-17
AGTACTT1581400.040.1860420-21
TATCAAC1612000.039.75199510-11
GTACTTT1577350.038.46093422-23
Produced by FastQC (version 0.11.5)