FastQCFastQC Report
Thu 26 May 2016
SRR1660599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31466306
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11574913.678509323592035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA468480.1488830624096772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1570000.082.179698
AGTGGTA1595300.081.291665
GTGGTAT1595500.081.16966
TGGTATC1594650.080.8239447
GTATCAA1601550.080.29119
GCAGTGG1676650.077.978223
AAGCAGT1679700.077.921941
CAGTGGT1722150.075.6668554
AGCAGTG1765700.074.315612
CAACGCA1526700.043.47258812-13
ATCAACG1537350.043.3007610-11
ACGCAGA1563950.042.5363114-15
TCAACGC1523350.041.98955512-13
AGAGTAC1587800.041.5435518-19
AACGCAG1545250.041.3291414-15
GAGTACT1492500.041.2756220-21
CGCAGAG1572500.040.58453816-17
AGTACTT1595650.040.35583520-21
TATCAAC1612700.039.80540510-11
GTACTTT1594550.038.60599522-23
Produced by FastQC (version 0.11.5)