##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660599_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31466306 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50431671261317 34.0 31.0 34.0 31.0 34.0 2 32.78519178577873 34.0 31.0 34.0 31.0 34.0 3 32.880065839313964 34.0 31.0 34.0 31.0 34.0 4 36.29302238400656 37.0 37.0 37.0 35.0 37.0 5 36.203688033797164 37.0 37.0 37.0 35.0 37.0 6 36.17695880793888 37.0 37.0 37.0 35.0 37.0 7 36.09644112658156 37.0 36.0 37.0 35.0 37.0 8 36.118910112931594 37.0 36.0 37.0 35.0 37.0 9 37.93603125832438 39.0 38.0 39.0 35.0 39.0 10-11 37.811376842264224 39.0 38.0 39.0 35.0 39.0 12-13 37.87612715010144 39.0 38.0 39.0 35.0 39.0 14-15 39.35861716656541 41.0 39.0 41.0 36.0 41.0 16-17 39.29606333517509 41.0 39.0 41.0 36.0 41.0 18-19 39.24384837228749 41.0 39.0 41.0 36.0 41.0 20-21 39.22684833103701 40.5 39.0 41.0 36.0 41.0 22-23 39.06449988123805 40.0 38.5 41.0 35.5 41.0 24-25 39.07639067960503 40.0 39.0 41.0 35.5 41.0 26-27 39.026591809029 40.0 39.0 41.0 35.0 41.0 28-29 38.93840198464987 40.0 38.5 41.0 35.0 41.0 30-31 38.82718578405739 40.0 38.0 41.0 35.0 41.0 32-33 38.69948774730659 40.0 38.0 41.0 35.0 41.0 34-35 38.785427053305845 40.0 38.0 41.0 35.0 41.0 36-37 38.72617252879954 40.0 38.0 41.0 35.0 41.0 38-39 38.433633089947065 40.0 38.0 41.0 34.0 41.0 40-41 38.319038227747484 40.0 38.0 41.0 34.0 41.0 42-43 38.21730874605999 40.0 38.0 41.0 34.0 41.0 44-45 38.10900407248312 40.0 37.0 41.0 33.5 41.0 46-47 37.99243017276956 40.0 37.0 41.0 33.0 41.0 48-49 37.842142401462695 40.0 37.0 41.0 33.0 41.0 50-51 37.645180006194565 40.0 36.5 41.0 33.0 41.0 52-53 37.2895377995752 40.0 36.0 41.0 32.0 41.0 54-55 37.08245167068546 40.0 36.0 41.0 31.0 41.0 56-57 36.71277929795763 40.0 36.0 41.0 30.5 41.0 58-59 36.39173122196168 39.0 35.0 41.0 29.5 41.0 60-61 36.11833260631229 39.0 35.0 41.0 29.0 41.0 62-63 35.93849616475477 39.0 35.0 41.0 29.0 41.0 64-65 35.66203223854748 38.0 35.0 40.0 28.0 41.0 66-67 35.317385300962876 37.0 35.0 40.0 28.0 41.0 68-69 35.02596270753866 37.0 34.5 40.0 28.0 41.0 70-71 34.72043122888336 36.0 34.0 39.0 28.0 41.0 72-73 34.289136370185936 36.0 34.0 39.0 26.0 41.0 74-75 33.94031787525361 35.0 34.0 38.5 26.0 40.0 76-77 32.27612969568147 34.5 31.5 36.0 24.5 39.0 78-79 33.26362908629949 35.0 33.5 37.0 26.0 39.0 80-81 33.15431320409837 35.0 34.0 36.5 26.0 39.0 82-83 32.93070967720202 35.0 34.0 36.0 26.0 37.5 84-85 32.66812507321323 35.0 33.0 36.0 25.5 37.0 86-87 32.4427025689002 35.0 33.0 35.0 25.0 37.0 88-89 32.19655114585106 35.0 33.0 35.0 25.0 36.0 90-91 32.039188584767466 35.0 33.0 35.0 24.5 36.0 92-93 31.89439405438948 35.0 33.0 35.0 24.0 36.0 94-95 31.651457991287568 35.0 32.5 35.0 23.5 35.5 96-97 30.614148225724367 35.0 31.5 35.0 11.5 35.0 98-99 31.171297720806503 35.0 31.5 35.0 20.0 35.0 100 31.336198980585774 35.0 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38646839904230745 1101 2 0.3225185968720439 1101 3 0.27419507329354076 1101 4 0.18371127018556876 1101 5 0.17046432081480134 1101 6 0.23351411027887536 1101 7 0.2581724179494742 1101 8 0.27639003478115853 1101 9 0.2798829786342907 1101 10-11 0.3096084065365474 1101 12-13 0.30102974617479816 1101 14-15 0.38650106617904356 1101 16-17 0.4052441231182087 1101 18-19 0.4049047661849059 1101 20-21 0.38571919978610936 1101 22-23 0.39440665522786134 1101 24-25 0.3535648253155941 1101 26-27 0.3752876708188424 1101 28-29 0.38203561292576893 1101 30-31 0.4005111850111831 1101 32-33 0.43160243330116543 1101 34-35 0.35791292367605365 1101 36-37 0.3915167518297906 1101 38-39 0.4265958426723202 1101 40-41 0.47961931692408655 1101 42-43 0.44504003163663697 1101 44-45 0.427182850503911 1101 46-47 0.42372027189644257 1101 48-49 0.4240108538465037 1101 50-51 0.46415070066044706 1101 52-53 0.5617875109355452 1101 54-55 0.5267619459762329 1101 56-57 0.47261467858585604 1101 58-59 0.5412893387304578 1101 60-61 0.540464712408145 1101 62-63 0.5009666865748343 1101 64-65 0.4915406902505026 1101 66-67 0.5256894094884288 1101 68-69 0.5186781412553998 1101 70-71 0.49308321907656705 1101 72-73 0.5306607212999026 1101 74-75 0.4822663964757723 1101 76-77 0.3787237952155138 1101 78-79 0.4445024046666788 1101 80-81 0.4728854501767117 1101 82-83 0.443564807788583 1101 84-85 0.4266412193855942 1101 86-87 0.45771248850207513 1101 88-89 0.5092994425291266 1101 90-91 0.4947265519272861 1101 92-93 0.4462524618899266 1101 94-95 0.5566154666591636 1101 96-97 1.45366121014143 1101 98-99 0.7434720138034514 1101 100 0.4719871065597303 1102 1 0.36476727562219224 1102 2 0.3022929979452016 1102 3 0.2491102362970068 1102 4 0.16619076545531897 1102 5 0.1546594112675237 1102 6 0.1914835811698623 1102 7 0.19354613137886645 1102 8 0.21413110286189863 1102 9 0.22071539650044514 1102 10-11 0.23593143288759677 1102 12-13 0.25930171433469695 1102 14-15 0.3201794844211534 1102 16-17 0.32729370226461185 1102 18-19 0.35281471815530097 1102 20-21 0.32275215965328385 1102 22-23 0.34505231658773283 1102 24-25 0.3357511060177174 1102 26-27 0.31021796367928545 1102 28-29 0.35334726237348235 1102 30-31 0.3553351883136102 1102 32-33 0.3600728356085767 1102 34-35 0.35798579385740936 1102 36-37 0.358901026423176 1102 38-39 0.3713412277573198 1102 40-41 0.3827651209185774 1102 42-43 0.3639129543093347 1102 44-45 0.39404704001843527 1102 46-47 0.36266680784717664 1102 48-49 0.3628109443336953 1102 50-51 0.4299705645028453 1102 52-53 0.49020384650143 1102 54-55 0.3915878714481096 1102 56-57 0.3853423688988329 1102 58-59 0.46300620489710553 1102 60-61 0.4936455326562239 1102 62-63 0.4133463011200078 1102 64-65 0.3762955241372339 1102 66-67 0.4073998094524143 1102 68-69 0.41515980673390374 1102 70-71 0.3819331922564544 1102 72-73 0.4433079963269648 1102 74-75 0.38996616141663054 1102 76-77 0.325428978409505 1102 78-79 0.40941920029020906 1102 80-81 0.4565718205144975 1102 82-83 0.4424113551846247 1102 84-85 0.42814398224700057 1102 86-87 0.4083527696197038 1102 88-89 0.458783807319854 1102 90-91 0.431278840275354 1102 92-93 0.42671576750385753 1102 94-95 0.5442379441383984 1102 96-97 1.446670203698826 1102 98-99 0.7632670289012431 1102 100 0.5824970046961617 1103 1 0.3581251044813101 1103 2 0.2759664353985869 1103 3 0.2288051702217686 1103 4 0.15746129101857775 1103 5 0.1452256354912862 1103 6 0.1836515733042745 1103 7 0.18685776295603063 1103 8 0.20842529300334434 1103 9 0.20512226789734456 1103 10-11 0.24162995997703263 1103 12-13 0.2417293938631886 1103 14-15 0.27753411462132505 1103 16-17 0.31054201861540776 1103 18-19 0.3233610160212166 1103 20-21 0.26340815344750723 1103 22-23 0.2696158389762857 1103 24-25 0.26058371038862305 1103 26-27 0.2762904659555332 1103 28-29 0.29460188875645343 1103 30-31 0.34015935219697724 1103 32-33 0.3172628835717646 1103 34-35 0.3148362425769733 1103 36-37 0.3182081367543006 1103 38-39 0.345280407783882 1103 40-41 0.3713962388498544 1103 42-43 0.3628737969319147 1103 44-45 0.3751014324283375 1103 46-47 0.3417040188964151 1103 48-49 0.33422070860659403 1103 50-51 0.36242169146448333 1103 52-53 0.45497263692691803 1103 54-55 0.40154686655846916 1103 56-57 0.3916638881596626 1103 58-59 0.4311175437751231 1103 60-61 0.41175311926037494 1103 62-63 0.3635882882027275 1103 64-65 0.37200755089790505 1103 66-67 0.44673041889902976 1103 68-69 0.42277653444523366 1103 70-71 0.37082924937911343 1103 72-73 0.45240014177462484 1103 74-75 0.4277317774713225 1103 76-77 0.3543550659153283 1103 78-79 0.3942286197131679 1103 80-81 0.39904118138575484 1103 82-83 0.3981464440663487 1103 84-85 0.42918403564970475 1103 86-87 0.41375365789857455 1103 88-89 0.473082967198053 1103 90-91 0.44445647916941056 1103 92-93 0.44830876421090693 1103 94-95 0.5840518139004693 1103 96-97 1.4539958144190557 1103 98-99 0.7930278583789097 1103 100 0.5786603799764549 1104 1 0.35138971184943557 1104 2 0.2905638688381984 1104 3 0.22794312879146617 1104 4 0.1595687088742821 1104 5 0.1343608781921688 1104 6 0.16295169460879322 1104 7 0.18037651478079653 1104 8 0.17603991866914015 1104 9 0.20443301251401635 1104 10-11 0.20136550338615677 1104 12-13 0.2171910026601651 1104 14-15 0.28062655724310304 1104 16-17 0.3099997932130094 1104 18-19 0.30849795756990517 1104 20-21 0.2676466823127228 1104 22-23 0.26210270600741126 1104 24-25 0.2648880519578469 1104 26-27 0.28002628131029894 1104 28-29 0.2952158966459777 1104 30-31 0.29894649544624485 1104 32-33 0.3306729012973051 1104 34-35 0.29269210636069687 1104 36-37 0.2850269333500748 1104 38-39 0.28535086304286494 1104 40-41 0.30358702885524025 1104 42-43 0.2879335003028629 1104 44-45 0.2751005943372604 1104 46-47 0.2805963046144626 1104 48-49 0.3085035147437978 1104 50-51 0.33032184053970326 1104 52-53 0.3991208233732877 1104 54-55 0.3196339028201578 1104 56-57 0.28868103656347444 1104 58-59 0.3506653659164698 1104 60-61 0.3683030566095695 1104 62-63 0.3141326052715172 1104 64-65 0.3051407528140544 1104 66-67 0.3850714994790465 1104 68-69 0.3209788628621979 1104 70-71 0.2664795456360025 1104 72-73 0.31754102781199833 1104 74-75 0.2608424253199999 1104 76-77 0.1852217730622101 1104 78-79 0.2994030414754434 1104 80-81 0.3570409384686073 1104 82-83 0.31353456855274686 1104 84-85 0.334020386457361 1104 86-87 0.31874137291325866 1104 88-89 0.3353593360360847 1104 90-91 0.33982459303777546 1104 92-93 0.3657287504137656 1104 94-95 0.4774549352734532 1104 96-97 1.381759042099869 1104 98-99 0.6728377281994433 1104 100 0.40404623135516005 1105 1 0.2932267846575911 1105 2 0.22473238371821225 1105 3 0.15870761351963836 1105 4 0.10671615055647266 1105 5 0.08815539604836431 1105 6 0.13436360239432332 1105 7 0.16067422717334523 1105 8 0.17631769289222632 1105 9 0.14788622178548394 1105 10-11 0.17288478064674706 1105 12-13 0.18952931428332676 1105 14-15 0.2561177894974591 1105 16-17 0.2500528699259803 1105 18-19 0.24860447188593326 1105 20-21 0.23118531507593332 1105 22-23 0.2034157713668563 1105 24-25 0.19631784234424998 1105 26-27 0.20222621041885702 1105 28-29 0.23238393419499204 1105 30-31 0.23001726215749585 1105 32-33 0.2357724974220332 1105 34-35 0.22995209883344359 1105 36-37 0.219537219214466 1105 38-39 0.23516906969923213 1105 40-41 0.24576107996044527 1105 42-43 0.2507809077973775 1105 44-45 0.2380102170117695 1105 46-47 0.22498017187906783 1105 48-49 0.2561170803102826 1105 50-51 0.2660361815123409 1105 52-53 0.37319165703953416 1105 54-55 0.27034041649093155 1105 56-57 0.27398907568490216 1105 58-59 0.32110987628092147 1105 60-61 0.3607126030092829 1105 62-63 0.29069263753856944 1105 64-65 0.2653856573169193 1105 66-67 0.3668618720861261 1105 68-69 0.33896982051449953 1105 70-71 0.2831511335163128 1105 72-73 0.2861748700016733 1105 74-75 0.2424173495411921 1105 76-77 0.16140234080577187 1105 78-79 0.2628879612220558 1105 80-81 0.31737390521996645 1105 82-83 0.24955870057198837 1105 84-85 0.2939211519112419 1105 86-87 0.2756168989592638 1105 88-89 0.31753850648874504 1105 90-91 0.31235615766669866 1105 92-93 0.3117192486861313 1105 94-95 0.457426885093394 1105 96-97 1.3438848255567102 1105 98-99 0.6551956518729334 1105 100 0.4416381576309689 1106 1 0.2820235868991432 1106 2 0.2083093603196886 1106 3 0.15155202107295906 1106 4 0.10765199573544493 1106 5 0.09259785048507752 1106 6 0.128100789955937 1106 7 0.1540569441757853 1106 8 0.16832330368454507 1106 9 0.16360216925147597 1106 10-11 0.17097202509503262 1106 12-13 0.16805491318343257 1106 14-15 0.20696525943014876 1106 16-17 0.22581841142730497 1106 18-19 0.22830110276402849 1106 20-21 0.17602114420761694 1106 22-23 0.20595872528304682 1106 24-25 0.18720478748907965 1106 26-27 0.18514895667542675 1106 28-29 0.18077452640599034 1106 30-31 0.18366768242660925 1106 32-33 0.21430677940155363 1106 34-35 0.20703929672466614 1106 36-37 0.1953227996547966 1106 38-39 0.2238726754368443 1106 40-41 0.24847623177203104 1106 42-43 0.24761050548492847 1106 44-45 0.268986131612607 1106 46-47 0.24573423452683585 1106 48-49 0.2685814299733025 1106 50-51 0.3146348130249166 1106 52-53 0.3959594965373441 1106 54-55 0.31051706004924284 1106 56-57 0.30983466213704247 1106 58-59 0.3709857597424886 1106 60-61 0.3791355134413692 1106 62-63 0.31292019583585784 1106 64-65 0.3662402628814476 1106 66-67 0.42666293120475984 1106 68-69 0.39278315215033643 1106 70-71 0.32639298071764244 1106 72-73 0.36475656295418446 1106 74-75 0.2970559297074544 1106 76-77 0.21623245965781024 1106 78-79 0.3361569862891187 1106 80-81 0.37542328584302 1106 82-83 0.3332415108586062 1106 84-85 0.3560030798403133 1106 86-87 0.35955930647715917 1106 88-89 0.43275076069555496 1106 90-91 0.3437764916723296 1106 92-93 0.38603529564313277 1106 94-95 0.5602691301371259 1106 96-97 1.414912214078992 1106 98-99 0.6844508092799373 1106 100 0.46005420063087854 1107 1 0.2600675728079409 1107 2 0.20047883499299246 1107 3 0.1415477107523344 1107 4 0.09625837851433516 1107 5 0.08150691280487621 1107 6 0.10528052557629053 1107 7 0.11713979008411712 1107 8 0.13134751356488295 1107 9 0.10645108629667277 1107 10-11 0.12290880101255652 1107 12-13 0.13328494088336384 1107 14-15 0.14966739934522622 1107 16-17 0.18048502725265791 1107 18-19 0.21144885814376124 1107 20-21 0.15238205261658777 1107 22-23 0.14838853331518465 1107 24-25 0.13681478925993673 1107 26-27 0.15152425752258836 1107 28-29 0.17715442997315023 1107 30-31 0.1925122330136233 1107 32-33 0.1825805485308365 1107 34-35 0.1610481912739843 1107 36-37 0.18581403111242167 1107 38-39 0.19788939196202904 1107 40-41 0.21993428629225775 1107 42-43 0.18707356603326986 1107 44-45 0.17718269000178566 1107 46-47 0.14628680556798912 1107 48-49 0.208943602310093 1107 50-51 0.1737635206223871 1107 52-53 0.27745954211820845 1107 54-55 0.15572347659786345 1107 56-57 0.1571291852646155 1107 58-59 0.2414789796329373 1107 60-61 0.2534075234377653 1107 62-63 0.22772696113740665 1107 64-65 0.1696078304290083 1107 66-67 0.23425761666212708 1107 68-69 0.2177677808509344 1107 70-71 0.1687299031523608 1107 72-73 0.23574847048613634 1107 74-75 0.15713709863970848 1107 76-77 0.07720901056236329 1107 78-79 0.18718408103340778 1107 80-81 0.20595170797567874 1107 82-83 0.19042500791638872 1107 84-85 0.21379808781795617 1107 86-87 0.20171086697204998 1107 88-89 0.2671965291786478 1107 90-91 0.282191672331777 1107 92-93 0.27358082989897525 1107 94-95 0.3721159019638556 1107 96-97 1.2535513044012738 1107 98-99 0.5857873135002798 1107 100 0.3129126871513215 1108 1 0.2217106519854255 1108 2 0.15683629877374017 1108 3 0.10080366805534169 1108 4 0.07298540207667514 1108 5 0.04838397790094717 1108 6 0.1004715238985483 1108 7 0.10040422793515091 1108 8 0.12271326711768182 1108 9 0.08948693368174787 1108 10-11 0.1060135179051116 1108 12-13 0.09991587043106875 1108 14-15 0.12985333819607803 1108 16-17 0.15327822225184917 1108 18-19 0.1440196904714739 1108 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-0.9784778726569883 2316 76-77 -0.9589183916728601 2316 78-79 -0.9411698914293325 2316 80-81 -0.8737543946114243 2316 82-83 -0.9025132035278247 2316 84-85 -0.914668206810731 2316 86-87 -0.8924226224502583 2316 88-89 -0.9607481732405638 2316 90-91 -0.967900165198806 2316 92-93 -1.0006689392120016 2316 94-95 -1.1171385239474212 2316 96-97 -0.33685159523235697 2316 98-99 -0.9858931087754641 2316 100 -1.2721739020326197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 17.0 8 62.0 9 40.0 10 49.0 11 64.0 12 259.0 13 1277.0 14 5496.0 15 15328.0 16 27762.0 17 38365.0 18 48189.0 19 57789.0 20 72785.0 21 99248.0 22 146687.0 23 231973.0 24 350592.0 25 431345.0 26 428425.0 27 384083.0 28 376968.0 29 415679.0 30 484310.0 31 567409.0 32 698200.0 33 892770.0 34 1241892.0 35 1935519.0 36 3443715.0 37 7068066.0 38 9372771.0 39 2623719.0 40 5453.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.636745080365976 33.73603192540685 27.79936179908574 20.827861195141434 2 21.46500595344068 32.00091476985295 27.46197956018206 19.072099716524317 3 24.88112700929033 26.73756021034288 27.00323938612503 21.378073394241753 4 20.796044195644683 27.379452815948813 26.51449482347255 25.310008164933954 5 21.43681244312567 30.963431169836074 26.442353926132924 21.157402460905324 6 25.846187044095153 26.719029614886036 26.56802665203089 20.866756688987923 7 21.320290980453823 26.903768113104853 30.829173275058093 20.94676763138323 8 25.257133773503632 27.051434000546486 26.676601314434556 21.01483091151532 9 25.081183663566993 26.70943008054393 27.076206530248577 21.1331797256405 10-11 20.8723038541607 29.322981223153427 28.904039450960656 20.900675471725215 12-13 20.909182984491412 27.09133858928341 28.744489423067325 23.254989003157853 14-15 20.83222209157098 31.556075865290804 26.538830431286804 21.07287161185141 16-17 23.160664065322962 27.038283685194404 26.552484623073912 23.24856762640872 18-19 21.07945008494222 29.157746483707413 26.590856711299683 23.171946720050684 20-21 23.17505090486422 29.166127928850095 26.573982946292485 21.0848382199932 22-23 23.179779984342616 27.17778184703346 28.667774031054044 20.97466413756988 24-25 21.03724126490225 29.156021019944983 26.441048607391306 23.36568910776146 26-27 21.039909686152516 26.949295962015963 30.8476797266508 21.16311462518072 28-29 21.140393391348734 27.076088181261987 30.685100102094943 21.098418325294336 30-31 21.20394439928677 26.971671300144184 30.73917809241364 21.085206208155412 32-33 21.145365622330925 27.095566029582475 30.57840146863145 21.180666879455153 34-35 21.12244670958671 27.146464180998947 30.569095278561864 21.161993830852474 36-37 21.147050209663444 27.114305463024095 30.558034110727906 21.180610216584558 38-39 21.315186322863866 27.02703776358569 30.526263335394756 21.13151257815569 40-41 21.220439244214333 27.284066800063737 30.507745863005347 20.98774809271658 42-43 21.256127870043645 27.117228041682484 30.51268045150345 21.113963636770425 44-45 21.310086755529138 27.16325350913541 30.42607663742768 21.10058309790777 46-47 21.39773337570248 27.284580519315277 30.360649477245477 20.95703662773677 48-49 21.419308140396403 27.389094254816985 30.197581665565572 20.994015939221043 50-51 21.53597985457646 27.35299282950452 29.973571462531407 21.13745585338761 52-53 21.676482781214233 27.499262027521226 29.717299969095418 21.106955222169123 54-55 21.769042053269843 27.7282143169102 29.16792259020431 21.334821039615644 56-57 22.097010656270765 28.083900587617723 28.40618690187753 21.412901854233983 58-59 22.26089255229274 28.40700461839444 27.857070828591528 21.475032000721296 60-61 22.331555601674225 28.577930410795773 27.539114638624255 21.551399348905747 62-63 22.23586553978196 28.678099720874357 27.57610722619983 21.509927513143854 64-65 22.300089353220155 28.904968142339005 27.252892656547917 21.542049847892926 66-67 22.211838063660387 28.94729498222915 27.19454429972946 21.646322654381002 68-69 22.111444267814555 28.880205770372562 27.26138488744428 21.746965074368607 70-71 22.032777508616366 28.8412720658569 27.312074856053293 21.813875569473442 72-73 22.018658368239176 28.7971540330691 27.25687307875248 21.927314519939245 74-75 21.98543227493312 28.839260710462117 27.09300150210566 22.082305512499108 76-77 21.960192328236396 28.888157728911374 26.963289295592258 22.18836064725998 78-79 22.00805022100118 28.971266834801668 26.6770948104138 22.343588133783353 80-81 22.007770603033876 29.07419720197966 26.43703372015566 22.4809984748308 82-83 21.924837470586773 29.210035680936446 26.23051173463587 22.634615113840912 84-85 21.89992760238148 29.241872804359108 26.040125192561188 22.81807440069823 86-87 21.834796171702404 29.27714044891452 25.922625524066728 22.965437855316345 88-89 21.83400273119987 29.30473274115435 25.799108618313255 23.062155909332528 90-91 21.8012515267788 29.272067433014293 25.707620249259417 23.219060790947495 92-93 21.76596160458468 29.232963967829562 25.679056080142814 23.32201834744294 94-95 21.762389270155012 29.22534465884007 25.628198060497017 23.384068010507907 96-97 22.304855240868896 28.9762426385482 25.447263076196563 23.271639044386337 98-99 21.644267021949563 29.11469693868856 25.640605280671576 23.6004307586903 100 21.61929203134774 29.112731586254597 25.626922998682826 23.641053383714837 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 46913.0 1 29808.0 2 9499.0 3 5761.5 4 5247.5 5 5355.0 6 5503.0 7 5646.0 8 5760.5 9 5976.0 10 6529.5 11 9413.0 12 74019.0 13 92693.5 14 46651.5 15 46911.0 16 53548.5 17 61675.5 18 70110.0 19 79267.0 20 91078.0 21 107531.5 22 129699.5 23 156288.0 24 188158.0 25 223922.5 26 263721.5 27 310302.5 28 364008.5 29 422241.5 30 477170.5 31 528560.5 32 582705.0 33 634354.5 34 681957.5 35 734741.0 36 794084.0 37 847625.5 38 898057.5 39 940396.0 40 967599.5 41 997670.0 42 1032862.0 43 1056866.5 44 1077639.5 45 1105369.0 46 1126736.5 47 1138327.0 48 1139143.5 49 1134335.0 50 1126739.0 51 1106385.0 52 1074847.5 53 1022795.5 54 950717.5 55 874603.0 56 795039.0 57 709012.5 58 617050.5 59 523507.0 60 428187.0 61 342209.0 62 268226.0 63 201912.0 64 149530.5 65 111230.5 66 82050.0 67 61117.0 68 47015.5 69 36811.0 70 29569.5 71 24342.5 72 20675.0 73 16996.5 74 13105.5 75 10508.5 76 8243.0 77 5977.5 78 4182.5 79 2710.5 80 1704.5 81 1128.0 82 758.5 83 482.0 84 318.0 85 236.0 86 154.5 87 90.5 88 54.0 89 35.0 90 23.5 91 15.5 92 9.5 93 6.0 94 4.5 95 3.5 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2809767374664188 2 0.001487305182883558 3 5.053024018771063E-4 4 1.2076409604610087E-4 5 0.0 6 1.1440809099104293E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.906801516517382E-5 16-17 1.271201011011588E-5 18-19 6.673805307810838E-4 20-21 3.336902653905419E-5 22-23 0.0 24-25 3.17800252752897E-6 26-27 1.1123008846351396E-4 28-29 2.0657016428938304E-5 30-31 6.038204802305043E-5 32-33 1.4301011373880366E-5 34-35 1.4618811626633264E-4 36-37 8.946077114994051E-4 38-39 5.92697471384153E-4 40-41 0.0011059448795800815 42-43 7.43652591441779E-4 44-45 0.009195550313405075 46-47 0.005038723007397182 48-49 0.004666896711676293 50-51 0.0022182457642152213 52-53 0.005459808342294771 54-55 0.001326816055243345 56-57 5.89519468856624E-4 58-59 0.0019354035392651428 60-61 0.005032367002342124 62-63 0.00629562300703489 64-65 0.01300915334643984 66-67 0.013941897088269591 68-69 0.011019723764206703 70-71 0.010676499491233575 72-73 0.001860720479868212 74-75 0.004689142729368995 76-77 0.015518186341923961 78-79 0.011731596330373193 80-81 0.010295139187930099 82-83 0.012225775723403949 84-85 0.010342809225843033 86-87 0.00496721795052778 88-89 0.011369304042234891 90-91 0.005648899492682745 92-93 0.008184945509650862 94-95 0.0160489127640213 96-97 0.4873752896193153 98-99 0.011691871298779083 100 0.015972640703360606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.1466306E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.38152058563718 #Duplication Level Percentage of deduplicated Percentage of total 1 77.15450411365057 38.87161237276515 2 11.866403665619954 11.956949211138246 3 3.9659191053140206 5.994271051360504 4 1.9690096045270218 3.9680679169518194 5 1.1501266123293812 2.8972563797580935 6 0.7740798960367111 2.339959333026087 7 0.5382588838160152 1.8982810724765062 8 0.39957360962520927 1.6104900831047868 9 0.3033493109466318 1.3754879598686927 >10 1.6908723113626911 15.092102826106673 >50 0.12094292114740396 4.213909615718541 >100 0.06620915578552311 5.107054069042795 >500 2.885028485783022E-4 0.10121281202551707 >1k 4.0567886838623925E-4 0.39695898243879174 >5k 1.887604085641652E-5 0.059710348433774436 >10k+ 3.775208111497421E-5 4.116675965783929 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1157491 3.678509323592035 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46848 0.1488830624096772 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.1754098495069615 3.1780025275289702E-6 0.0 3.1780025275289702E-6 0.0 2 0.17544798553729185 1.2712010110115881E-5 0.0 6.3560050550579405E-6 0.0 3 0.1754734095575121 1.5890012637644852E-5 0.0 9.534007582586911E-6 3.1780025275289702E-6 4 0.17548929957014972 1.9068015165173822E-5 0.0 1.5890012637644852E-5 6.3560050550579405E-6 5 0.17550201158025985 3.4958027802818675E-5 0.0 1.5890012637644852E-5 9.534007582586911E-6 6 0.17551472359036996 4.4492035385405584E-5 0.0 1.5890012637644852E-5 1.9068015165173822E-5 7 0.17552425759795257 5.0848040440463524E-5 0.0 1.9068015165173822E-5 1.9068015165173822E-5 8 0.17553061360300762 5.720404549552146E-5 0.0 1.9068015165173822E-5 1.9068015165173822E-5 9 0.17554332561311772 6.356005055057941E-5 0.0 2.5424020220231762E-5 1.9068015165173822E-5 10-11 0.17556398262954667 8.262806571575323E-5 0.0 2.7013021483996247E-5 1.9068015165173822E-5 12-13 0.1755989406573495 9.216207329834014E-5 0.0 4.1314032857876614E-5 2.3835018956467277E-5 14-15 0.1756783907205377 1.0328508214469153E-4 0.0 4.4492035385405584E-5 3.1780025275289705E-5 16-17 0.17573082776224191 1.1281908972727844E-4 0.0 4.608103664917007E-5 4.1314032857876614E-5 18-19 0.17581981183301276 1.2076409604610087E-4 3.1780025275289702E-6 4.7670037912934554E-5 5.720404549552146E-5 20-21 0.17592627491768498 1.4142111247503918E-4 3.1780025275289702E-6 5.561504423175698E-5 6.991605560563735E-5 22-23 0.17625360917802047 1.5890012637644852E-4 3.1780025275289702E-6 5.720404549552146E-5 7.46830593969308E-5 24-25 0.17755182321051605 1.6525613143150646E-4 3.1780025275289702E-6 5.720404549552146E-5 7.627206066069529E-5 26-27 0.18151638136360843 1.8591314786044476E-4 3.1780025275289702E-6 5.720404549552146E-5 7.627206066069529E-5 28-29 0.19274585329463204 1.9862515797056064E-4 3.1780025275289702E-6 5.879304675928595E-5 7.945006318822426E-5 30-31 0.21785842926716598 2.1769317313573446E-4 3.1780025275289702E-6 6.038204802305043E-5 8.58060682432822E-5 32-33 0.26599245554911977 2.3676118830090828E-4 3.1780025275289702E-6 6.51490518143439E-5 9.692907708963359E-5 34-35 0.3456490889016334 2.621852085211401E-4 3.1780025275289702E-6 6.991605560563735E-5 1.0169608088092705E-4 36-37 0.4642457872239595 2.780752211587849E-4 3.1780025275289702E-6 7.786106192445977E-5 1.0169608088092705E-4 38-39 0.6261967960268358 2.8602022747760734E-4 3.1780025275289702E-6 7.945006318822426E-5 1.0487408340845602E-4 40-41 0.8330958835778182 2.987322375877232E-4 3.1780025275289702E-6 8.262806571575323E-5 1.064630846722205E-4 42-43 1.0885373707355417 3.114442476978391E-4 3.1780025275289702E-6 9.057307203457565E-5 1.0805208593598499E-4 44-45 1.3911928524434993 3.1938925401666155E-4 3.1780025275289702E-6 9.851807835339808E-5 1.2076409604610087E-4 46-47 1.742203231609074 3.3527926665430634E-4 3.1780025275289702E-6 1.0328508214469153E-4 1.2394209857362985E-4 48-49 2.1416431912916627 3.5116927929195125E-4 3.1780025275289702E-6 1.0805208593598499E-4 1.2553109983739433E-4 50-51 2.5871006275728714 3.654702906658316E-4 3.1780025275289702E-6 1.1123008846351396E-4 1.3347610615621676E-4 52-53 3.077698411755101 3.76593299512183E-4 3.1780025275289702E-6 1.1281908972727844E-4 1.3347610615621676E-4 54-55 3.610919565836549 3.924833121498278E-4 3.1780025275289702E-6 1.1917509478233638E-4 1.3665410868374573E-4 56-57 4.180449398795016 4.051953222599437E-4 3.1780025275289702E-6 1.302981036286878E-4 1.4142111247503918E-4 58-59 4.78944048913781 4.1314032857876613E-4 3.1780025275289702E-6 1.3347610615621676E-4 1.493661187938616E-4 60-61 5.435035812592682 4.369753475352334E-4 3.1780025275289702E-6 1.3665410868374573E-4 1.509551200576261E-4 62-63 6.109891005318515 4.592213652279362E-4 3.1780025275289702E-6 1.4301011373880367E-4 1.5254412132139058E-4 64-65 6.809656970856382 4.687553728105231E-4 3.1780025275289702E-6 1.509551200576261E-4 1.5254412132139058E-4 66-67 7.536571022985666 4.7828938039311004E-4 3.1780025275289702E-6 1.5731112511268403E-4 1.5254412132139058E-4 68-69 8.289282510632166 4.957683942945194E-4 3.1780025275289702E-6 1.60489127640213E-4 1.5254412132139058E-4 70-71 9.060410522925697 5.005353980858129E-4 3.1780025275289702E-6 1.700231352227999E-4 1.5254412132139058E-4 72-73 9.847175896655934 5.259594183060446E-4 3.1780025275289702E-6 1.7796814154162234E-4 1.5254412132139058E-4 74-75 10.654456865702635 5.418494309436894E-4 3.1780025275289702E-6 1.811461440691513E-4 1.5413312258515506E-4 76-77 11.473712548273063 5.545614410538053E-4 3.1780025275289702E-6 1.811461440691513E-4 1.60489127640213E-4 78-79 12.303986365606436 5.656844499001567E-4 3.1780025275289702E-6 1.8750214912420925E-4 1.6525613143150646E-4 80-81 13.151435697599839 5.736294562189791E-4 3.1780025275289702E-6 1.9862515797056064E-4 1.716121364865644E-4 82-83 14.006122930349688 5.879304675928596E-4 3.1780025275289702E-6 2.12926169344441E-4 1.7479013901409337E-4 84-85 14.863389112150628 5.942864726479174E-4 3.1780025275289702E-6 2.145151706082055E-4 1.811461440691513E-4 86-87 15.711912608998336 6.085874840217977E-4 3.1780025275289702E-6 2.2087117566326343E-4 1.922691529155027E-4 88 16.34676151690637 6.101764852855623E-4 3.1780025275289702E-6 2.2246017692702791E-4 1.9703615670679616E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 157000 0.0 82.17969 8 AGTGGTA 159530 0.0 81.29166 5 GTGGTAT 159550 0.0 81.1696 6 TGGTATC 159465 0.0 80.823944 7 GTATCAA 160155 0.0 80.2911 9 GCAGTGG 167665 0.0 77.97822 3 AAGCAGT 167970 0.0 77.92194 1 CAGTGGT 172215 0.0 75.666855 4 AGCAGTG 176570 0.0 74.31561 2 CAACGCA 152670 0.0 43.472588 12-13 ATCAACG 153735 0.0 43.30076 10-11 ACGCAGA 156395 0.0 42.53631 14-15 TCAACGC 152335 0.0 41.989555 12-13 AGAGTAC 158780 0.0 41.54355 18-19 AACGCAG 154525 0.0 41.32914 14-15 GAGTACT 149250 0.0 41.27562 20-21 CGCAGAG 157250 0.0 40.584538 16-17 AGTACTT 159565 0.0 40.355835 20-21 TATCAAC 161270 0.0 39.805405 10-11 GTACTTT 159455 0.0 38.605995 22-23 >>END_MODULE