FastQCFastQC Report
Thu 26 May 2016
SRR1660598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28058989
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12650204.5084304356083535No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2197570.783196429493593Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2093100.745964154303635Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA615820.219473338829136Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG366560.13063906187068963Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2547450.086.489558
GTGGTAT2545300.086.484046
TGGTATC2554250.086.053687
AGTGGTA2565250.085.930895
GTATCAA2574250.085.555259
AAGCAGT2612550.085.19311
GCAGTGG2650950.083.585453
CAGTGGT2659650.082.9391864
AGCAGTG2722500.081.62652
CAACGCA2527500.044.81082512-13
ATCAACG2546750.044.503710-11
ACGCAGA2563500.044.1759614-15
TCAACGC2521100.043.7954812-13
AGAGTAC2479950.043.6894818-19
AACGCAG2553300.043.2264614-15
TATCAAC2581400.042.69188710-11
CGCAGAG2576050.042.66185816-17
GAGTACT1729150.041.97039820-21
GCAGAGT2672950.041.8522616-17
AGTACTT1791650.041.76835320-21
Produced by FastQC (version 0.11.5)