FastQCFastQC Report
Thu 26 May 2016
SRR1660598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28058989
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9249783.2965478549494422No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2235910.7968604998562137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2165490.7717633732277381Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1621700.5779609521925397TruSeq Adapter, Index 27 (97% over 44bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT618120.22029304049408197TruSeq Adapter, Index 1 (100% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG413080.147218419024292Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC348590.12423469712326413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1668850.083.27598
GTGGTAT1682500.082.960616
AGTGGTA1689750.082.7025
TGGTATC1680900.082.611827
GTATCAA1703250.082.054799
AAGCAGT1759850.080.661281
GCAGTGG1773200.079.1402363
CAGTGGT1791800.078.2147454
AGCAGTG1856300.075.992932
CAACGCA1662350.043.71750312-13
ATCAACG1671100.043.5251510-11
ACGCAGA1704550.042.839214-15
TCAACGC1652750.042.33359512-13
AGAGTAC1678500.041.8398418-19
AACGCAG1688500.041.5138214-15
TATCAAC1716950.040.79445310-11
CGCAGAG1719700.040.60761316-17
GAGTACT1273750.039.99234420-21
AGTACTT1340400.039.72180620-21
GCAGAGT1824600.039.42431616-17
Produced by FastQC (version 0.11.5)