FastQCFastQC Report
Thu 26 May 2016
SRR1660597_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660597_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28021044
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11869774.236019899900946No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG3463051.2358747232972476Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA3372421.2035311746414588Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA1073360.3830549639763601Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG831730.29682334462627447Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC575770.20547771167983608Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG544820.19443244156070702Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA338200.12069500336961035Illumina Single End PCR Primer 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2302250.085.8612756
GGTATCA2305750.085.742398
TGGTATC2311400.085.386527
AGTGGTA2328750.084.993875
GTATCAA2342500.084.397929
AAGCAGT2377650.084.221951
GCAGTGG2417350.082.454213
CAGTGGT2426600.081.707874
AGCAGTG2487550.080.351372
CAACGCA2292200.044.5525112-13
ATCAACG2307250.044.3121610-11
ACGCAGA2328400.043.855914-15
TCAACGC2284200.043.37076612-13
AGAGTAC2263000.043.2634118-19
AACGCAG2318750.042.73267714-15
TATCAAC2338450.042.2736510-11
CGCAGAG2343100.042.15673416-17
GAGTACT1633400.041.38617320-21
GCAGAGT2442200.041.32388716-17
AGTACTT1700950.041.1796420-21
Produced by FastQC (version 0.11.5)