##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660597_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28021044
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40556411816776	34.0	31.0	34.0	31.0	34.0
2	32.5343740939845	34.0	31.0	34.0	31.0	34.0
3	32.55411022515792	34.0	31.0	34.0	31.0	34.0
4	35.95706601795422	37.0	37.0	37.0	35.0	37.0
5	35.955032831753165	37.0	37.0	37.0	35.0	37.0
6	35.98657009353399	37.0	37.0	37.0	35.0	37.0
7	35.85713469491001	37.0	35.0	37.0	35.0	37.0
8	35.93672783926252	37.0	36.0	37.0	35.0	37.0
9	37.67021728383853	39.0	38.0	39.0	35.0	39.0
10-11	37.52954925590923	39.0	37.5	39.0	35.0	39.0
12-13	37.629326730295986	39.0	38.0	39.0	35.0	39.0
14-15	39.09480754892644	41.0	39.0	41.0	36.0	41.0
16-17	39.00443545215518	41.0	39.0	41.0	36.0	41.0
18-19	38.82318686983968	40.0	39.0	41.0	35.0	41.0
20-21	38.635260645534835	40.0	38.5	41.0	34.0	41.0
22-23	38.373394064118386	40.0	38.0	41.0	33.5	41.0
24-25	38.32131274266583	40.0	38.0	41.0	34.0	41.0
26-27	38.24960524311657	40.0	38.0	41.0	33.5	41.0
28-29	38.149822076579305	40.0	38.0	41.0	33.0	41.0
30-31	37.96098741003369	40.0	38.0	41.0	32.5	41.0
32-33	37.99225407161846	40.0	38.0	41.0	33.0	41.0
34-35	37.92704549123866	40.0	38.0	41.0	33.0	41.0
36-37	37.72408197924389	40.0	38.0	41.0	32.0	41.0
38-39	37.340925662869665	40.0	36.0	41.0	31.0	41.0
40-41	37.27711041030449	40.0	36.0	41.0	31.0	41.0
42-43	37.15595245130767	40.0	36.0	41.0	31.0	41.0
44-45	36.99809594888755	40.0	35.0	41.0	30.5	41.0
46-47	36.91679398169462	40.0	35.0	41.0	30.5	41.0
48-49	36.864599209793894	40.0	35.0	41.0	30.5	41.0
50-51	35.65572315935124	38.5	34.0	40.0	28.5	40.5
52-53	35.72411333068104	38.5	35.0	39.5	28.0	40.5
54-55	36.115984811272554	39.0	35.0	41.0	27.5	41.0
56-57	35.806260269959964	39.0	35.0	41.0	26.0	41.0
58-59	35.53227808000301	39.0	35.0	41.0	25.5	41.0
60-61	35.29187840039079	38.5	35.0	41.0	25.5	41.0
62-63	34.955192872185634	38.0	34.0	40.5	25.0	41.0
64-65	34.61294083118388	37.0	34.0	40.0	23.5	41.0
66-67	34.33030204013812	36.5	34.0	40.0	23.5	41.0
68-69	33.988787444893205	36.0	33.0	39.0	23.0	41.0
70-71	33.67158850326919	35.5	33.0	39.0	23.0	41.0
72-73	33.350933873127644	35.0	33.0	38.5	23.0	40.5
74-75	32.99030086102431	35.0	33.0	37.5	21.5	40.0
76-77	32.63880846124077	35.0	33.0	37.0	20.5	39.0
78-79	32.306677224446034	35.0	32.0	36.5	20.0	39.0
80-81	31.908960619026185	35.0	32.0	36.0	19.5	38.5
82-83	31.39405917566812	35.0	31.5	35.5	17.5	37.0
84-85	30.92409597586728	35.0	31.0	35.0	15.0	37.0
86-87	30.536457795790906	35.0	31.0	35.0	10.5	36.5
88-89	30.265492445606238	35.0	30.0	35.0	10.0	36.0
90-91	29.990979493840413	34.5	30.0	35.0	10.0	36.0
92-93	29.753932562255713	34.0	29.5	35.0	10.0	35.5
94-95	29.553488478159487	34.0	29.0	35.0	10.0	35.0
96-97	29.339672354820184	34.0	29.0	35.0	10.0	35.0
98-99	29.174232337667362	34.0	29.0	35.0	9.0	35.0
100	29.041143292162847	34.0	28.0	35.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19758384061481848
1101	2	0.20735498374335748
1101	3	0.20263226416062707
1101	4	0.13651942852177967
1101	5	0.15464096058029497
1101	6	0.16457338895585139
1101	7	0.1952787496104449
1101	8	0.13157249439512952
1101	9	0.1823193916347492
1101	10-11	0.1889251228305966
1101	12-13	0.20180353937725926
1101	14-15	0.26578342665543886
1101	16-17	0.31556901245316027
1101	18-19	0.3274488562747564
1101	20-21	0.3176881884445848
1101	22-23	0.28948276524857874
1101	24-25	0.3056083308069546
1101	26-27	0.29088162317718513
1101	28-29	0.2948418871613825
1101	30-31	0.3188446086483694
1101	32-33	0.2900473498128804
1101	34-35	0.3008638283706162
1101	36-37	0.24635918017563085
1101	38-39	0.2390815378323694
1101	40-41	0.23875751729320882
1101	42-43	0.27145440425388756
1101	44-45	0.2090038932616025
1101	46-47	0.19878713469876175
1101	48-49	0.21425363339894687
1101	50-51	0.1686974632330589
1101	52-53	0.259272960832746
1101	54-55	0.28943596645932246
1101	56-57	0.31149714812476503
1101	58-59	0.3124733695362636
1101	60-61	0.29209918591917017
1101	62-63	0.23600872238353787
1101	64-65	0.23586967420188643
1101	66-67	0.21439105449270102
1101	68-69	0.245359797872716
1101	70-71	0.2106260837486147
1101	72-73	0.22184866224823452
1101	74-75	0.21350191559036347
1101	76-77	0.1434423853778597
1101	78-79	0.11206044904869827
1101	80-81	0.08766522061663906
1101	82-83	0.03766198841545432
1101	84-85	0.05644478130640351
1101	86-87	0.047387010342241354
1101	88-89	0.0981648476146404
1101	90-91	0.07884137489415721
1101	92-93	0.0670512785615216
1101	94-95	0.1572786616311852
1101	96-97	0.1678865731088024
1101	98-99	0.17389219467705175
1101	100	0.14305179178224492
1102	1	0.17577202869442488
1102	2	0.15687347169558308
1102	3	0.14991684643713654
1102	4	0.134130827827029
1102	5	0.1256488171725607
1102	6	0.13396473335256331
1102	7	0.15418922947980462
1102	8	0.14082973107448993
1102	9	0.18057805926128623
1102	10-11	0.1708821402121785
1102	12-13	0.19301740034817527
1102	14-15	0.21460434569193154
1102	16-17	0.2196414299836178
1102	18-19	0.22493391777609162
1102	20-21	0.2218341801521433
1102	22-23	0.24440681146707277
1102	24-25	0.22892490954917122
1102	26-27	0.25987550049336505
1102	28-29	0.24890434972868292
1102	30-31	0.2812320808264701
1102	32-33	0.22537443901913434
1102	34-35	0.14835182229028732
1102	36-37	0.11898665387700191
1102	38-39	0.15713965856288326
1102	40-41	0.15382639246895025
1102	42-43	0.20436614937551667
1102	44-45	0.21422877441987254
1102	46-47	0.23254039100171298
1102	48-49	0.21528171969710286
1102	50-51	0.21507953073859198
1102	52-53	0.2620443831024133
1102	54-55	0.2746531480291239
1102	56-57	0.36281934205003097
1102	58-59	0.3035666911956554
1102	60-61	0.25297187826101464
1102	62-63	0.2352255875573519
1102	64-65	0.20983644373207966
1102	66-67	0.2491361764197677
1102	68-69	0.20983232895809323
1102	70-71	0.2345680471426803
1102	72-73	0.22302711625804506
1102	74-75	0.17649117540916848
1102	76-77	0.14787972313078512
1102	78-79	0.131424487787541
1102	80-81	0.10441664379323967
1102	82-83	0.012828855126787886
1102	84-85	-0.029901178973858578
1102	86-87	0.009914663063767648
1102	88-89	0.044818598371751506
1102	90-91	0.01236314098489899
1102	92-93	-0.00637822648308628
1102	94-95	0.06308775416480472
1102	96-97	0.053672728425400607
1102	98-99	0.11778139311741143
1102	100	0.12750618964181726
1103	1	0.09550039742750727
1103	2	0.11499270227265868
1103	3	0.11932152311701572
1103	4	0.10784439906634447
1103	5	0.12001676411661322
1103	6	0.09639694749445482
1103	7	0.08937841846078953
1103	8	0.09522648675326906
1103	9	0.1456890114967777
1103	10-11	0.1462967155486652
1103	12-13	0.14422707202673024
1103	14-15	0.1413128062943727
1103	16-17	0.17313111745831833
1103	18-19	0.16395859853814443
1103	20-21	0.18601417212200744
1103	22-23	0.1851768049529383
1103	24-25	0.15753736664299822
1103	26-27	0.1961211407771799
1103	28-29	0.1857213802861537
1103	30-31	0.1692331785520551
1103	32-33	0.2039951487567464
1103	34-35	0.19086368367896256
1103	36-37	0.1447828763557979
1103	38-39	0.145605693908621
1103	40-41	0.15208627875892944
1103	42-43	0.17164635374659554
1103	44-45	0.16191277316277564
1103	46-47	0.1569141149229054
1103	48-49	0.15485133711337795
1103	50-51	0.06884228597657227
1103	52-53	0.16703472574455702
1103	54-55	0.25018326385727363
1103	56-57	0.26520382766413775
1103	58-59	0.23515443098253286
1103	60-61	0.2190542444022725
1103	62-63	0.18664453535298264
1103	64-65	0.23414021087485537
1103	66-67	0.21356687533376117
1103	68-69	0.18393893172521558
1103	70-71	0.20306971299967103
1103	72-73	0.15133109537131872
1103	74-75	0.17730545987537738
1103	76-77	0.10845345428885622
1103	78-79	0.09964669716612917
1103	80-81	0.12453061300630353
1103	82-83	0.06017440155069664
1103	84-85	0.09048675897513903
1103	86-87	0.13640549212677655
1103	88-89	0.11168744247773787
1103	90-91	0.14344487948122264
1103	92-93	0.08505176915360124
1103	94-95	0.13532532633820438
1103	96-97	0.11462959812171647
1103	98-99	0.107466279373714
1103	100	0.09416293137851284
1104	1	0.0938903612868458
1104	2	0.08928881429328328
1104	3	0.11581617919161857
1104	4	0.11364041382769585
1104	5	0.07413155413885875
1104	6	0.08369817357048248
1104	7	0.09848004103889707
1104	8	0.07023356560281968
1104	9	0.10807608444966377
1104	10-11	0.10881251984285001
1104	12-13	0.1013085582854174
1104	14-15	0.13058929317222834
1104	16-17	0.1298559217957802
1104	18-19	0.13076526881763328
1104	20-21	0.16027222028868238
1104	22-23	0.1454475447475474
1104	24-25	0.1372354820731161
1104	26-27	0.16106709776418882
1104	28-29	0.1512070573102804
1104	30-31	0.14987502344073533
1104	32-33	0.17544890736498076
1104	34-35	0.1296915109010044
1104	36-37	0.11560762617291687
1104	38-39	0.1535370737552313
1104	40-41	0.0878777656878853
1104	42-43	0.12846416851696318
1104	44-45	0.1284507977003031
1104	46-47	0.13513944576529724
1104	48-49	0.14693349678868373
1104	50-51	0.11293286724351503
1104	52-53	0.2076289473811883
1104	54-55	0.24375225678231516
1104	56-57	0.33300010678183156
1104	58-59	0.27002914854720217
1104	60-61	0.2044565806902625
1104	62-63	0.20870443560369978
1104	64-65	0.18993680326239826
1104	66-67	0.197038255019244
1104	68-69	0.2048126932599601
1104	70-71	0.17419285045031785
1104	72-73	0.14612948139181725
1104	74-75	0.17502494931294166
1104	76-77	0.1637842267466567
1104	78-79	0.11110163733982859
1104	80-81	0.18322238592059747
1104	82-83	0.10615045924237876
1104	84-85	0.11682745054526578
1104	86-87	0.15096079358087522
1104	88-89	0.10833172266006841
1104	90-91	0.09497121929869579
1104	92-93	0.07713037836525771
1104	94-95	0.16266674852886354
1104	96-97	0.13002009658716673
1104	98-99	0.16352580251832904
1104	100	0.13147924151825308
1105	1	0.05299768567097374
1105	2	-0.012707522760315726
1105	3	0.03582599108435858
1105	4	0.04497882082115723
1105	5	0.04816258673136531
1105	6	0.042822407155533426
1105	7	0.04577937824435452
1105	8	0.028846462445713428
1105	9	0.018190027120091656
1105	10-11	0.03284699350616194
1105	12-13	0.0929040404339716
1105	14-15	0.07943884928918266
1105	16-17	0.06755896996087785
1105	18-19	0.06768165043840213
1105	20-21	0.07033286822180429
1105	22-23	0.08974995959273713
1105	24-25	0.055818654887211494
1105	26-27	0.07509325801230204
1105	28-29	0.09063666217961242
1105	30-31	0.022055394963508945
1105	32-33	0.06147740472778196
1105	34-35	0.10624154742209413
1105	36-37	0.04832637270006046
1105	38-39	0.07432200507041387
1105	40-41	0.053552616026216526
1105	42-43	0.1163733281092334
1105	44-45	0.10191498763204976
1105	46-47	0.08055663627405352
1105	48-49	0.04494368934063431
1105	50-51	-0.03339057841036208
1105	52-53	0.04104228902776441
1105	54-55	0.12232209222924695
1105	56-57	0.2325399114024833
1105	58-59	0.19787720088433502
1105	60-61	0.1649902366094551
1105	62-63	0.1408273207412023
1105	64-65	0.05661416817375908
1105	66-67	0.16679682545969143
1105	68-69	0.15373760324608554
1105	70-71	0.12670570322279673
1105	72-73	0.1028919597826814
1105	74-75	0.0760483811199606
1105	76-77	0.09123234999876928
1105	78-79	0.13578138211443047
1105	80-81	0.08774808257811273
1105	82-83	0.017925783585656774
1105	84-85	0.033460459700911827
1105	86-87	0.11462115524561511
1105	88-89	0.11568304617580338
1105	90-91	0.0688464639887556
1105	92-93	0.0563641952476317
1105	94-95	0.06437698349733267
1105	96-97	0.07886426784407874
1105	98-99	0.10800839517939664
1105	100	0.12131738697723549
1106	1	0.008185532461261857
1106	2	0.010087715342507408
1106	3	-0.021499900774649916
1106	4	-0.02304580310919846
1106	5	0.02790991774248397
1106	6	0.04680925259295776
1106	7	0.02677447624619589
1106	8	0.024687413577879624
1106	9	0.07022388241998812
1106	10-11	0.043294144952170655
1106	12-13	0.02585712226998993
1106	14-15	0.0674939573239115
1106	16-17	0.01167313959933125
1106	18-19	0.0297793542539182
1106	20-21	0.013326437727883444
1106	22-23	0.014292710399892883
1106	24-25	-0.014409533359099669
1106	26-27	0.01864728444046193
1106	28-29	0.014822450217387484
1106	30-31	0.06987919415801969
1106	32-33	-0.0038406281804412856
1106	34-35	0.06413230047324703
1106	36-37	0.07040575499009094
1106	38-39	0.021274157556909756
1106	40-41	0.06996288221950664
1106	42-43	0.042157756923622
1106	44-45	0.03307718151192063
1106	46-47	0.037099161544070114
1106	48-49	-0.023268268528859437
1106	50-51	-0.17753738462169366
1106	52-53	-0.05257165648660589
1106	54-55	0.008775413039280977
1106	56-57	0.10634583205958137
1106	58-59	0.08527831531245056
1106	60-61	0.0777852653367006
1106	62-63	0.12650208989726508
1106	64-65	0.08059429839406107
1106	66-67	0.020397302245349636
1106	68-69	0.11701045450676162
1106	70-71	0.08555338877610552
1106	72-73	0.09300357364907796
1106	74-75	0.08518471153470841
1106	76-77	0.04339876218523386
1106	78-79	0.02471550259354416
1106	80-81	0.031103535531105564
1106	82-83	0.019352420382237057
1106	84-85	0.042903924771117374
1106	86-87	0.08900125893239164
1106	88-89	0.12432871039916193
1106	90-91	0.10674763310955271
1106	92-93	0.06846216301376273
1106	94-95	0.11135481221168675
1106	96-97	0.14740308094090793
1106	98-99	0.21016875705546312
1106	100	0.16595929204635596
1107	1	-0.05507081208006781
1107	2	-0.09250073004503179
1107	3	-0.08876099906370882
1107	4	-0.0463396896478514
1107	5	-0.05112740546374539
1107	6	-0.05353917304420719
1107	7	-0.11032170412182296
1107	8	-0.07881917210656297
1107	9	-0.1142314871705068
1107	10-11	-0.08827196266433646
1107	12-13	-0.09589199113072056
1107	14-15	-0.11122546912263687
1107	16-17	-0.12483776195986707
1107	18-19	-0.10718008486728792
1107	20-21	-0.05706758691117386
1107	22-23	-0.10350302534389044
1107	24-25	-0.11288066272407349
1107	26-27	-0.09431602450489862
1107	28-29	-0.08175946779531529
1107	30-31	-0.12477172357135657
1107	32-33	-0.16501437066040126
1107	34-35	-0.1164696945966881
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2312	86-87	0.011405819221010915
2312	88-89	0.06151900057615478
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2312	92-93	0.06218147100240756
2312	94-95	0.03038740053782618
2312	96-97	0.092585692654918
2312	98-99	0.07535587797471521
2312	100	0.08511420369949363
2313	1	-0.1727189989215816
2313	2	-0.15657392002115245
2313	3	-0.16512490564039695
2313	4	-0.11923938510236098
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2313	24-25	-0.12766362171672796
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2313	30-31	-0.13521490574027695
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2313	66-67	-0.15661737807238296
2313	68-69	-0.1342590895475837
2313	70-71	-0.15100244259532047
2313	72-73	-0.09567091860268562
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2313	80-81	-0.09267586103876013
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2313	84-85	-5.700135445039223E-4
2313	86-87	-0.021049618240727597
2313	88-89	-0.024363631014743703
2313	90-91	-0.01977350559974056
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2313	98-99	-0.017873717658382304
2313	100	-0.036935708217914254
2314	1	-0.07908728033361712
2314	2	-0.12400040415957392
2314	3	-0.1012643112145426
2314	4	-0.022962426370497724
2314	5	-0.014712349336498676
2314	6	-0.6380360880301907
2314	7	-0.26913769387907394
2314	8	-0.14829558008077726
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2314	10-11	-0.12233600999294936
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2314	14-15	-0.08876004467013843
2314	16-17	-0.040452653909724745
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2314	22-23	-0.08167880236620562
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2314	80-81	0.056431582799216784
2314	82-83	0.13073884430928118
2314	84-85	0.17611373186882417
2314	86-87	0.17646900228324824
2314	88-89	0.2134638371248414
2314	90-91	0.2760857838076589
2314	92-93	0.2421251952769552
2314	94-95	0.2178945867284412
2314	96-97	-2.0920995616790385
2314	98-99	-2.105301016450632
2314	100	-0.9706079405957766
2315	1	-0.28869221710704807
2315	2	-0.2968193096439862
2315	3	-0.2816353428797669
2315	4	-0.23573000786296916
2315	5	-0.2452802449653575
2315	6	-0.27008390982007313
2315	7	-0.23137637692065027
2315	8	-0.25562380054108047
2315	9	-0.3275801342339122
2315	10-11	-0.35695169901959645
2315	12-13	-0.334565003046869
2315	14-15	-0.41785968036074905
2315	16-17	-0.39171518724916865
2315	18-19	-0.3605982266365544
2315	20-21	-0.33647093806260386
2315	22-23	-0.3621049776311125
2315	24-25	-0.34718496518760844
2315	26-27	-0.3557940669005859
2315	28-29	-0.3743972561800959
2315	30-31	-0.3915272154975611
2315	32-33	-0.3602030360973174
2315	34-35	-0.3180657008321788
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2315	66-67	-0.48220561369540604
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2315	86-87	-0.27254826835732615
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2315	92-93	-0.2578173707571949
2315	94-95	-0.3090261079316967
2315	96-97	-0.2133803339060698
2315	98-99	-0.24466332903756438
2315	100	-0.26375865337790927
2316	1	-0.4765369312102692
2316	2	-0.4309400040950706
2316	3	-0.41779863172471465
2316	4	-0.3612551072132746
2316	5	-0.35993217784476883
2316	6	-0.33164366971391246
2316	7	-0.34611335330512816
2316	8	-0.36140574013538895
2316	9	-0.4706246385631587
2316	10-11	-0.4870056972159631
2316	12-13	-0.4691964014632859
2316	14-15	-0.5724860470364561
2316	16-17	-0.5240633668752182
2316	18-19	-0.5499743882333803
2316	20-21	-0.6087593126154616
2316	22-23	-0.5998844031268078
2316	24-25	-0.5850431223657253
2316	26-27	-0.56096074774117
2316	28-29	-0.5708096604840023
2316	30-31	-0.6185371212941604
2316	32-33	-0.5160933175736062
2316	34-35	-0.4852071937354836
2316	36-37	-0.4385011546304298
2316	38-39	-0.4478688232097525
2316	40-41	-0.5457863046216929
2316	42-43	-0.5219262481785449
2316	44-45	-0.5597828875697957
2316	46-47	-0.5784723495141293
2316	48-49	-0.5919179786859488
2316	50-51	-0.6032481233538505
2316	52-53	-0.635949697657928
2316	54-55	-0.7580468864183914
2316	56-57	-0.6984990514662286
2316	58-59	-0.7505779666726937
2316	60-61	-0.7792442609456032
2316	62-63	-0.7878857416981973
2316	64-65	-0.7103570825800176
2316	66-67	-0.7116434672682104
2316	68-69	-0.7033254580258372
2316	70-71	-0.667046571966516
2316	72-73	-0.6130767088731446
2316	74-75	-0.6178131288691446
2316	76-77	-0.5882639976152788
2316	78-79	-0.5939855241074987
2316	80-81	-0.6789516165830385
2316	82-83	-0.624945228335168
2316	84-85	-0.5869537329832113
2316	86-87	-0.8372870829217405
2316	88-89	-0.6996459102597612
2316	90-91	-0.9037861137450101
2316	92-93	-0.8910226294577654
2316	94-95	-0.8461010897270711
2316	96-97	-0.7566861919861765
2316	98-99	-0.98854517969054
2316	100	-1.1314801252683715
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	174.0
3	411.0
4	618.0
5	1275.0
6	11492.0
7	2046.0
8	3063.0
9	10938.0
10	22785.0
11	22897.0
12	24018.0
13	28141.0
14	33964.0
15	42359.0
16	54318.0
17	70639.0
18	92089.0
19	117907.0
20	148373.0
21	184855.0
22	236233.0
23	323055.0
24	445846.0
25	542268.0
26	541171.0
27	493146.0
28	464099.0
29	503238.0
30	598110.0
31	691338.0
32	697761.0
33	790643.0
34	1094598.0
35	1718332.0
36	3077820.0
37	5821255.0
38	7038252.0
39	2067140.0
40	4377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.36221385612591	44.55688146323477	16.573037698477147	19.507866982162177
2	20.86047922919819	35.070952628257615	27.64660372020316	16.421964422341034
3	27.320794727626918	31.441411918316163	21.675684727462027	19.562108626594892
4	19.571309216755477	25.562512558115298	28.025591253020636	26.840586972108582
5	20.562966319316224	38.1250970518057	22.940550422345545	18.371386206532524
6	27.445995508717573	24.165936047513004	28.9761200850233	19.411948358746127
7	20.307328061008082	29.848591509711227	30.284296211673883	19.55978421760681
8	26.667448706390427	23.861502311394894	29.876921175035264	19.594127807179408
9	26.606692726559167	28.511196863962347	25.052638506123138	19.82947190335535
10-11	19.54753614688449	30.79730297024657	30.372663643248504	19.282497239620433
12-13	19.69176914675429	27.054405876018123	30.055365951266694	23.198459025960897
14-15	19.682995173663336	34.291656382446604	26.309223076524574	19.716125367365485
16-17	24.066298549978864	26.260215762733587	26.21162879981475	23.46185688747279
18-19	20.80679638362786	29.769056288280954	25.69654380156893	23.727603526522255
20-21	25.099359201223482	29.58203042178247	25.165825796544027	20.152784580450025
22-23	25.754640537239105	25.58783746227994	28.51954291695893	20.137979083522026
24-25	21.93953869771392	30.02891033602202	24.253199661994756	23.7783513042693
26-27	21.600707654938102	27.63644450791097	30.668383953701756	20.09446388344917
28-29	24.44057828068186	25.496496603340223	29.50508891350228	20.557836202475634
30-31	24.35923966954662	24.758347164598398	30.014187417611453	20.86822574824353
32-33	23.299615652905707	25.270859394174554	30.429411833505004	21.000113119414728
34-35	23.35449235717659	25.245236737509163	30.371991417629356	21.028279487684888
36-37	22.913752708949385	25.56016740966115	30.881990846627982	20.644089034761485
38-39	23.33355505558428	25.844094377251693	30.30333836032735	20.51901220683668
40-41	24.148700863148527	26.07981954355344	29.444803870429798	20.32667572286823
42-43	23.226693520849786	27.01637208435598	29.51832407127215	20.23861032352209
44-45	23.143276845283893	26.998397663811446	29.646759319395837	20.21156617150882
46-47	23.55177821797173	26.155025284859295	29.933964061040136	20.359232436128842
48-49	23.319352847718008	25.71776214642872	30.320970717706956	20.641914288146314
50-51	23.206436951534286	26.13216847147994	30.141947044463446	20.51944753252233
52-53	22.782886584327898	26.680438095828656	29.850357645431018	20.686317674412425
54-55	22.58473496262692	26.26754867423946	29.60426358860112	21.5434527745325
56-57	23.306768671691145	25.970745044409725	28.752603178327142	21.969883105571988
58-59	23.987435698050326	26.578786814615064	27.78832971555161	21.645447771783
60-61	24.380572110167247	26.57745903946674	27.524299164661226	21.51766968570479
62-63	23.783045077651952	26.55773506887166	27.54688312984478	22.112336723631604
64-65	23.265990029528496	26.48369999030199	27.5867199400841	22.663590040085417
66-67	23.6101332066187	26.422753491549845	27.28043265119752	22.686680650633946
68-69	22.91869872313594	26.877790715438344	28.185496230712147	22.01801433071357
70-71	22.320912603803134	27.0365160701627	28.8087893043668	21.833782021667364
72-73	22.077859126129702	27.348063194990413	28.791815606020577	21.78226207285931
74-75	21.976731836771133	28.36515106066057	28.576711967771494	21.081405134796803
76-77	22.00159525186967	29.320190992953727	27.74673835047084	20.93147540470576
78-79	22.090779793745124	30.344902417499746	26.770183473211496	20.79413431554363
80-81	22.186683800648286	30.772254602160302	26.419255096529305	20.62180650066211
82-83	22.307718762481553	30.993528235517974	26.0849306861446	20.613822315855877
84-85	22.51201091039014	30.99436071531049	25.862879479506685	20.630748894792692
86-87	22.789622342209388	30.868288696290474	25.696641412744903	20.645447548755232
88-89	23.113112564201593	30.639026000869347	25.626377502140997	20.621483932788067
90-91	23.51322289246823	30.16138646231073	25.514397597221123	20.81099304799992
92-93	23.789866132302347	29.94360612803896	25.442189633651534	20.82433810600715
94-95	24.13332304233561	29.804890826431524	25.28209554619929	20.779690585033578
96-97	24.29796854434909	29.51605953224815	25.326710519574323	20.859261403828434
98-99	24.716099403560847	29.295831593702033	25.184165504913487	20.80390349782363
100	24.980214494875337	29.13147418155865	25.14697344628071	20.7413378772853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5744.0
1	6133.5
2	6037.5
3	5433.5
4	5543.5
5	5990.5
6	6725.5
7	7745.5
8	8620.5
9	8791.5
10	9015.0
11	12153.0
12	88286.0
13	106565.5
14	51339.5
15	55077.0
16	64677.5
17	73498.0
18	79694.5
19	86606.0
20	95564.5
21	107653.5
22	123095.0
23	143087.5
24	165422.0
25	189759.0
26	217189.5
27	252272.5
28	293743.5
29	335630.5
30	390186.0
31	472222.5
32	573377.5
33	666355.0
34	739054.5
35	786048.5
36	805269.0
37	796362.0
38	782841.0
39	777031.0
40	782259.5
41	790556.0
42	802979.0
43	812286.0
44	819657.0
45	839739.0
46	850641.5
47	860388.5
48	868486.5
49	873772.0
50	877398.0
51	881312.5
52	886003.5
53	876801.5
54	854250.5
55	827038.5
56	791455.0
57	744503.0
58	681091.5
59	595131.5
60	493948.5
61	401537.5
62	325062.5
63	254668.0
64	195609.0
65	149616.0
66	112369.0
67	84872.5
68	65351.5
69	50524.0
70	39026.5
71	30403.0
72	24375.5
73	19298.5
74	15341.0
75	12921.0
76	9830.5
77	6089.0
78	3621.5
79	2407.5
80	1608.5
81	1090.0
82	780.0
83	540.0
84	366.5
85	256.0
86	182.0
87	147.0
88	118.5
89	88.0
90	69.0
91	57.0
92	42.0
93	26.5
94	21.0
95	23.0
96	16.0
97	10.0
98	5.5
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02089144144664988
2	0.010145946025422892
3	0.0074444049979008635
4	0.004689332774324897
5	0.004336026880368911
6	0.005103307357142011
7	0.004100489617731588
8	0.0010777614138859353
9	7.38730505544333E-4
10-11	0.001573817163985753
12-13	0.003013806337836663
14-15	0.009976430571252092
16-17	0.00789049829835034
18-19	0.09880431292995365
20-21	0.09468062646059869
22-23	0.09512850413424996
24-25	0.09354398073105342
26-27	0.0848754957167192
28-29	0.07953843547014165
30-31	0.06445869754174756
32-33	0.07016690741429905
34-35	0.07442620624699064
36-37	0.0791619327245623
38-39	0.07332524798148134
40-41	0.06996884198889948
42-43	0.06940319568392955
44-45	0.09049805567558439
46-47	0.0876002335958646
48-49	0.0913349267072276
50-51	0.08898490720045976
52-53	0.08978965951447063
54-55	0.07784684967483724
56-57	0.07629087624286947
58-59	0.07466174350962797
60-61	0.06670879214921471
62-63	0.076347976185327
64-65	0.07302547328357929
66-67	0.07537727716354894
68-69	0.07634083869251981
70-71	0.07661206341919309
72-73	0.09281774083792167
74-75	0.09880788167635725
76-77	0.10430018239149119
78-79	0.09649533400682715
80-81	0.09554426309026888
82-83	0.07036140409329503
84-85	0.06558999015168743
86-87	0.07201194930495808
88-89	0.06958877049691653
90-91	0.07125180632099218
92-93	0.07911375464811375
94-95	0.08016475046397271
96-97	0.08091240283552605
98-99	0.07358219772254025
100	0.0740443503818059
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8021044E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.99124307285595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.24907999887843	35.067899723100574
2	11.896339267192442	10.942548618292172
3	4.407019410722074	6.0805290283594
4	2.1402378917138294	3.9372880444618987
5	1.2905851469772636	2.967780760042443
6	0.8391233419174668	2.315539535174006
7	0.5639002653294082	1.8154131920128942
8	0.4101022886804169	1.508889123474848
9	0.30148467047228406	1.2479089286187626
>10	1.695299442918347	13.920323945109846
>50	0.12843617903054086	4.144668159584515
>100	0.07543231928564567	5.527451495634863
>500	0.001561513929377358	0.4999223554153723
>1k	0.0010806258575854456	1.0168014514167494
>5k	2.0917564790236804E-4	0.7039563077356462
>10k+	1.0846144704524756E-4	8.303079331565916
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1186977	4.236019899900946	No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG	346305	1.2358747232972476	Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	337242	1.2035311746414588	Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA	107336	0.3830549639763601	Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG	83173	0.29682334462627447	Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC	57577	0.20547771167983608	Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG	54482	0.19443244156070702	Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA	33820	0.12069500336961035	Illumina Single End PCR Primer 1 (100% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6051848746249425E-4	3.568746403595812E-6	1.3204361693304502E-4	3.568746403595812E-6	7.137492807191624E-6
2	2.8193096588406913E-4	1.0706239210787435E-5	1.3204361693304502E-4	3.568746403595812E-6	7.137492807191624E-6
3	3.247559227272189E-4	1.7843732017979058E-5	1.3204361693304502E-4	7.137492807191624E-6	7.137492807191624E-6
4	3.425996547451979E-4	3.5687464035958116E-5	1.3204361693304502E-4	1.4274985614383248E-5	7.137492807191624E-6
5	4.496620468530723E-4	3.925621043955393E-5	1.3204361693304502E-4	1.4274985614383248E-5	7.137492807191624E-6
6	4.996244965034136E-4	5.353119605393718E-5	1.3204361693304502E-4	1.4274985614383248E-5	1.0706239210787435E-5
7	6.352368598400545E-4	6.06686888611288E-5	1.3204361693304502E-4	1.4274985614383248E-5	1.4274985614383248E-5
8	7.315930127371414E-4	7.494367447551205E-5	1.3204361693304502E-4	1.4274985614383248E-5	1.4274985614383248E-5
9	0.014567622819478104	8.92186600898953E-5	1.3204361693304502E-4	2.141247842157487E-5	1.4274985614383248E-5
10-11	0.07418710023794974	9.635615289708692E-5	2.2839976983013197E-4	2.141247842157487E-5	1.9628105219776965E-5
12-13	0.30247088580996484	1.1419988491506597E-4	3.729339991757623E-4	2.141247842157487E-5	2.498122482517068E-5
14-15	0.7371263540359168	1.3561236333664085E-4	4.960557500998178E-4	2.676559802696859E-5	3.211871763236231E-5
16-17	1.6771163130110356	1.4631860254742828E-4	9.546396629618797E-4	2.8549971228766496E-5	3.5687464035958116E-5
18-19	3.1026199452097503	1.7665294697799267E-4	0.0013793204849897813	2.8549971228766496E-5	3.747183723775602E-5
20-21	4.030427988336195	2.2126227702294032E-4	0.0016915857953044148	3.0334344430564403E-5	3.925621043955393E-5
22-23	4.377192370134389	2.533809946553026E-4	0.0021287572297449017	3.5687464035958116E-5	4.282495684314974E-5
24-25	4.62813055787643	2.85499712287665E-4	0.0027336597451543916	3.747183723775602E-5	4.6393703246745554E-5
26-27	4.797747721319734	2.962059514984524E-4	0.003128006222751729	4.460933004494765E-5	5.1746822852139266E-5
28-29	4.89651456241245	3.247559227272189E-4	0.0032921685573171363	4.6393703246745554E-5	6.780618166832042E-5
30-31	4.948407703867137	3.7293399917576235E-4	0.003654396317282111	4.817807644854346E-5	6.959055487011832E-5
32-33	4.9801124469166815	4.0683709000992254E-4	0.003797146173425944	5.531556925573508E-5	7.672804767730995E-5
34-35	5.002038824820374	4.264651952296995E-4	0.0040112709576416924	6.06686888611288E-5	9.10030332916932E-5
36-37	5.019782631939053	4.3538706123868906E-4	0.0043520862391850925	6.06686888611288E-5	1.0349364570427855E-4
38-39	5.037333726751937	4.4430892724767857E-4	0.0045001892149343185	6.06686888611288E-5	1.0349364570427855E-4
40-41	5.058278699394641	4.567995396602639E-4	0.004776767061212994	6.423743526472461E-5	1.0706239210787435E-4
42-43	5.084046832801804	4.657214056692534E-4	0.005133641701572576	7.137492807191623E-5	1.1241551171326807E-4
44-45	5.116252984721054	4.728588984764451E-4	0.005338844619779334	7.672804767730996E-5	1.1776863131866179E-4
46-47	5.155013139410508	4.871338840908283E-4	0.0057528192025964485	7.851242087910786E-5	1.213373777222576E-4
48-49	5.202065276368717	5.049776161088074E-4	0.006120400082166817	8.029679408090576E-5	1.2669049732765133E-4
50-51	5.2733135139433065	5.406650801447655E-4	0.006123968828570413	8.208116728270367E-5	1.3561236333664085E-4
52-53	5.355251931369866	5.460181997501592E-4	0.0062149718618621065	8.386554048450158E-5	1.4631860254742828E-4
54-55	5.4371635831984	5.799212905843194E-4	0.006529021545378538	8.92186600898953E-5	1.4988734895102408E-4
56-57	5.535989308606775	6.138243814184797E-4	0.006614671459064837	8.92186600898953E-5	1.6416233456540734E-4
58-59	5.653197646740072	6.298837402346608E-4	0.006714596358365519	9.278740649349111E-5	1.7308420057439688E-4
60-61	5.791741735247266	6.459430990508419E-4	0.006837718109289575	9.992489930068274E-5	1.784373201797906E-4
62-63	5.952397776471141	6.566493382616294E-4	0.006866268080518342	1.0527801890607646E-4	1.9271230579417383E-4
64-65	6.139244847551005	6.816305630868001E-4	0.007083961611137686	1.1776863131866179E-4	2.1055603781215289E-4
66-67	6.3581267707227465	6.959055487011834E-4	0.0071749646444293795	1.213373777222576E-4	2.2483102342653615E-4
68-69	6.610326510318459	7.155336539209603E-4	0.007435483131891874	1.249061241258534E-4	2.2661539662833407E-4
70-71	6.899402106502527	7.369461323425352E-4	0.0076656672749238035	1.284748705294492E-4	2.319685162337278E-4
72-73	7.227080832534291	7.60142983965908E-4	0.007690648499748974	1.3918110974023667E-4	2.426747554445152E-4
74-75	7.595844751537451	7.904773283964723E-4	0.007690648499748974	1.427498561438325E-4	2.444591286463131E-4
76-77	8.01078646462994	8.15458553221643E-4	0.00791547952317551	1.4631860254742828E-4	2.5873411426069633E-4
78-79	8.473740307463206	8.368710316432179E-4	0.008293766641956666	1.4631860254742828E-4	2.7479347307687754E-4
80-81	8.983300193954229	8.600678832665907E-4	0.008557853875822757	1.481029757492262E-4	2.8371533908586705E-4
82-83	9.54599336127519	8.939709741007508E-4	0.009087812716756734	1.534560953546199E-4	2.854997122876649E-4
84-85	10.163324036035203	9.028928401097404E-4	0.009171678257241236	1.534560953546199E-4	2.890684586912607E-4
86-87	10.828996949578324	9.29658438136709E-4	0.009350115577421027	1.552404685564178E-4	3.015590711038461E-4
88	11.361535994162102	9.67130275374465E-4	0.009353684323824623	1.5702484175821572E-4	3.1048093711283565E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGTAT	230225	0.0	85.861275	6
GGTATCA	230575	0.0	85.74239	8
TGGTATC	231140	0.0	85.38652	7
AGTGGTA	232875	0.0	84.99387	5
GTATCAA	234250	0.0	84.39792	9
AAGCAGT	237765	0.0	84.22195	1
GCAGTGG	241735	0.0	82.45421	3
CAGTGGT	242660	0.0	81.70787	4
AGCAGTG	248755	0.0	80.35137	2
CAACGCA	229220	0.0	44.55251	12-13
ATCAACG	230725	0.0	44.31216	10-11
ACGCAGA	232840	0.0	43.8559	14-15
TCAACGC	228420	0.0	43.370766	12-13
AGAGTAC	226300	0.0	43.26341	18-19
AACGCAG	231875	0.0	42.732677	14-15
TATCAAC	233845	0.0	42.27365	10-11
CGCAGAG	234310	0.0	42.156734	16-17
GAGTACT	163340	0.0	41.386173	20-21
GCAGAGT	244220	0.0	41.323887	16-17
AGTACTT	170095	0.0	41.17964	20-21
>>END_MODULE