FastQCFastQC Report
Thu 26 May 2016
SRR1660597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28021044
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9031203.223006252015449No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3523251.2573585766468944Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3396761.2122175033878109Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1072890.38288723289539106TruSeq Adapter, Index 5 (100% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG925550.33030532338481033Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT850820.30363608151073884TruSeq Adapter, Index 25 (97% over 44bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC703890.2512004906027056Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTG575380.2053385305700958TruSeq Adapter, Index 5 (97% over 37bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT526930.18804795424467413Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA361940.12916720733174683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1617150.082.8375558
GTGGTAT1624350.082.71346
AGTGGTA1635100.082.247195
TGGTATC1627850.082.151597
GTATCAA1651550.081.561729
AAGCAGT1696400.080.5153961
GCAGTGG1721050.078.392773
CAGTGGT1729100.077.930874
AGCAGTG1795450.075.5634842
CAACGCA1614850.043.5105512-13
ATCAACG1626900.043.2460510-11
ACGCAGA1654550.042.62701814-15
TCAACGC1606000.041.9857912-13
AGAGTAC1632200.041.69791818-19
AACGCAG1642000.041.12680414-15
TATCAAC1669050.040.39973410-11
CGCAGAG1668950.040.27262516-17
GAGTACT1268050.039.59391820-21
AGTACTT1331200.039.5797220-21
GCAGAGT1775150.039.2240516-17
Produced by FastQC (version 0.11.5)