##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660597_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28021044 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59424159927803 34.0 31.0 34.0 31.0 34.0 2 32.827935925585074 34.0 31.0 34.0 31.0 34.0 3 32.806998340247425 34.0 31.0 34.0 31.0 34.0 4 36.26296275756178 37.0 37.0 37.0 35.0 37.0 5 36.158480640478636 37.0 37.0 37.0 35.0 37.0 6 36.20590535456138 37.0 37.0 37.0 35.0 37.0 7 36.09860214344619 37.0 35.0 37.0 35.0 37.0 8 36.159995430577105 37.0 36.0 37.0 35.0 37.0 9 37.85843882190828 39.0 38.0 39.0 35.0 39.0 10-11 37.797658306378594 39.0 38.0 39.0 35.0 39.0 12-13 37.831824538728824 39.0 38.0 39.0 35.0 39.0 14-15 39.34090535670263 41.0 39.0 41.0 36.0 41.0 16-17 39.303673142942145 41.0 39.0 41.0 36.0 41.0 18-19 39.221262776647436 41.0 39.0 41.0 36.0 41.0 20-21 39.089812481647726 40.5 39.0 41.0 36.0 41.0 22-23 38.862580780359224 40.0 38.5 41.0 34.5 41.0 24-25 38.8154260954731 40.0 38.0 41.0 34.5 41.0 26-27 38.65696936559537 40.0 38.0 41.0 34.0 41.0 28-29 38.432352859515156 40.0 38.0 41.0 34.0 41.0 30-31 38.40982655749728 40.0 38.0 41.0 34.0 41.0 32-33 38.320531115828516 40.0 38.0 41.0 34.0 41.0 34-35 38.347141259262145 40.0 38.0 41.0 34.0 41.0 36-37 38.30824592045892 40.0 38.0 41.0 34.0 41.0 38-39 38.03221853546927 40.0 37.5 41.0 33.0 41.0 40-41 37.93515075669558 40.0 37.0 41.0 33.0 41.0 42-43 37.798240511666876 40.0 37.0 41.0 33.0 41.0 44-45 37.650261085918146 40.0 37.0 41.0 33.0 41.0 46-47 37.463833556665485 40.0 36.5 41.0 32.0 41.0 48-49 37.31293339748512 40.0 36.0 41.0 32.0 41.0 50-51 37.2522708468678 40.0 36.0 41.0 31.5 41.0 52-53 37.05015933738943 40.0 36.0 41.0 31.0 41.0 54-55 36.670999267550485 39.5 35.0 41.0 30.0 41.0 56-57 36.321350268034266 39.0 35.0 41.0 28.5 41.0 58-59 36.08771903716364 39.0 35.0 41.0 28.0 41.0 60-61 35.906385358090155 39.0 35.0 41.0 28.0 41.0 62-63 35.64709940857307 38.0 35.0 40.0 28.0 41.0 64-65 35.34728939792536 37.5 35.0 40.0 28.0 41.0 66-67 35.03573187708495 37.0 34.0 40.0 27.0 41.0 68-69 34.72237106868681 36.5 34.0 39.0 26.0 41.0 70-71 34.36547726415904 36.0 34.0 39.0 26.0 41.0 72-73 33.94086342750113 35.0 34.0 38.5 26.0 40.0 74-75 33.52962801814236 35.0 33.0 37.5 25.5 40.0 76-77 31.72085131803083 34.0 30.5 35.5 22.5 38.5 78-79 32.8042765108966 35.0 33.0 36.0 25.0 39.0 80-81 32.73599124286733 35.0 33.0 36.0 25.0 38.5 82-83 32.525591658897504 35.0 33.0 36.0 25.0 37.0 84-85 32.27345162442913 35.0 33.0 35.5 24.0 37.0 86-87 31.9959822874551 35.0 33.0 35.0 23.0 36.5 88-89 31.691185239208075 35.0 32.5 35.0 20.5 36.0 90-91 31.340237679937978 35.0 32.0 35.0 19.5 36.0 92-93 31.038525420394755 35.0 32.0 35.0 17.5 36.0 94-95 30.680341549729555 35.0 31.5 35.0 14.0 35.0 96-97 30.51190888890507 35.0 31.0 35.0 11.5 35.0 98-99 30.401886168124214 35.0 31.0 35.0 10.0 35.0 100 30.304847635227297 35.0 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2010862266786475 1101 2 0.19505254471609135 1101 3 0.25531114329118765 1101 4 0.16472587167749708 1101 5 0.11769479783480108 1101 6 0.13995440238858947 1101 7 0.14397176988212124 1101 8 0.15487835268040584 1101 9 0.29099467217837116 1101 10-11 0.21429520036343774 1101 12-13 0.28547811478907903 1101 14-15 0.3357226039083443 1101 16-17 0.29436590634595206 1101 18-19 0.28620643243029775 1101 20-21 0.23247096855462956 1101 22-23 0.2713419183871437 1101 24-25 0.3226832999148499 1101 26-27 0.4003845987419723 1101 28-29 0.5390430368117123 1101 30-31 0.43398746055327564 1101 32-33 0.406633779221103 1101 34-35 0.41361654209593723 1101 36-37 0.3968987089328877 1101 38-39 0.38586810770438973 1101 40-41 0.3640165185716526 1101 42-43 0.3853644943454242 1101 44-45 0.4256240664673001 1101 46-47 0.4121055279874213 1101 48-49 0.4082981693115215 1101 50-51 0.40163000979045904 1101 52-53 0.39027549654559124 1101 54-55 0.3864836653078427 1101 56-57 0.3751347129774061 1101 58-59 0.45563991782058366 1101 60-61 0.40013260674149365 1101 62-63 0.3428501358405498 1101 64-65 0.3829284902611363 1101 66-67 0.3286460683704391 1101 68-69 0.2969426751337707 1101 70-71 0.2546877754522896 1101 72-73 0.24372316121719706 1101 74-75 0.23384924030565202 1101 76-77 0.21496488452298834 1101 78-79 0.3732838367384872 1101 80-81 0.3623500328730742 1101 82-83 0.2755470950259422 1101 84-85 0.2729471791971463 1101 86-87 0.35160149720308453 1101 88-89 0.33533880869855537 1101 90-91 0.34881179879888435 1101 92-93 0.27086172987653967 1101 94-95 0.44099502143879477 1101 96-97 0.39619586875851454 1101 98-99 0.35414440814339443 1101 100 0.400239037864786 1102 1 0.20162742040578507 1102 2 0.17906670829667348 1102 3 0.25608825737158014 1102 4 0.1443419219176576 1102 5 0.10816911550600139 1102 6 0.11961524830437043 1102 7 0.12346880507116964 1102 8 0.1256009353899472 1102 9 0.25144167291316677 1102 10-11 0.18215297289912513 1102 12-13 0.25554243592578274 1102 14-15 0.24653513855044196 1102 16-17 0.25198558143997474 1102 18-19 0.22701650204969326 1102 20-21 0.23037471736287785 1102 22-23 0.22968912016840903 1102 24-25 0.24063947764287974 1102 26-27 0.303132538444423 1102 28-29 0.46147323571825183 1102 30-31 0.37081684028012063 1102 32-33 0.36366217229533504 1102 34-35 0.3241179136483652 1102 36-37 0.3413340935094311 1102 38-39 0.33926833633349673 1102 40-41 0.3192264075753073 1102 42-43 0.3230407193358076 1102 44-45 0.3258982359717635 1102 46-47 0.3372226032968655 1102 48-49 0.3131466841958854 1102 50-51 0.3282403843083799 1102 52-53 0.3222663677680728 1102 54-55 0.3457599278756547 1102 56-57 0.3840241782877385 1102 58-59 0.37964735641055114 1102 60-61 0.2808345092673079 1102 62-63 0.2698344534065953 1102 64-65 0.28473183963353677 1102 66-67 0.2790645848407607 1102 68-69 0.2556507451589951 1102 70-71 0.29603598530211883 1102 72-73 0.19939567736183506 1102 74-75 0.25189852563317316 1102 76-77 0.2075176913565322 1102 78-79 0.3275279185029305 1102 80-81 0.30106756989908234 1102 82-83 0.23509909945826735 1102 84-85 0.2338033816616587 1102 86-87 0.33627744358056333 1102 88-89 0.31594018633143506 1102 90-91 0.27021343538686793 1102 92-93 0.24574748581036587 1102 94-95 0.3980969936659413 1102 96-97 0.368262145924394 1102 98-99 0.30536751518857486 1102 100 0.31070464983611146 1103 1 0.14430491276002044 1103 2 0.12907889277369122 1103 3 0.1805280520930097 1103 4 0.10853012194122158 1103 5 0.07886402675747206 1103 6 0.10677105311489754 1103 7 0.1063229256333571 1103 8 0.08674090998776762 1103 9 0.2140911103264287 1103 10-11 0.14375100148370024 1103 12-13 0.187393856473129 1103 14-15 0.1933672754053788 1103 16-17 0.14913900216746612 1103 18-19 0.1552616522979946 1103 20-21 0.14437254113919806 1103 22-23 0.19418180562087883 1103 24-25 0.1755432711260667 1103 26-27 0.26581827408168834 1103 28-29 0.33372688119303007 1103 30-31 0.2961006762319087 1103 32-33 0.2776671621089051 1103 34-35 0.2712726723945451 1103 36-37 0.24339829267734814 1103 38-39 0.21388634875697932 1103 40-41 0.18855784362279593 1103 42-43 0.25019865586848766 1103 44-45 0.24726391613948095 1103 46-47 0.2174484952596245 1103 48-49 0.2353184312888601 1103 50-51 0.24575965603103356 1103 52-53 0.16123902929800238 1103 54-55 0.20206892699725643 1103 56-57 0.20376185338199804 1103 58-59 0.2103859106631063 1103 60-61 0.18037309299865 1103 62-63 0.1117603144706365 1103 64-65 0.11400189544116301 1103 66-67 0.09703688167441982 1103 68-69 0.07528981292420411 1103 70-71 0.06393815521931145 1103 72-73 0.08559257341272541 1103 74-75 0.10612384446475431 1103 76-77 0.043017769028661945 1103 78-79 0.17140366589358536 1103 80-81 0.1580822750565929 1103 82-83 0.1912624335349591 1103 84-85 0.1758972088200892 1103 86-87 0.1731239330024792 1103 88-89 0.23762185838284822 1103 90-91 0.16351536882736184 1103 92-93 0.14609672513424599 1103 94-95 0.2817337169668832 1103 96-97 0.21916643973991157 1103 98-99 0.1986369342371752 1103 100 0.2128481671696889 1104 1 0.09640804071669606 1104 2 0.09693344545303262 1104 3 0.13701068597329424 1104 4 0.10832832223822919 1104 5 0.07672150217128149 1104 6 0.09002530032099543 1104 7 0.08807059576774634 1104 8 0.08615875109644833 1104 9 0.17533462759882923 1104 10-11 0.13887979689554442 1104 12-13 0.18950130896661221 1104 14-15 0.17360036190218864 1104 16-17 0.10368441657154648 1104 18-19 0.12797176589823778 1104 20-21 0.13004206333383195 1104 22-23 0.11730258891588363 1104 24-25 0.11547129151315261 1104 26-27 0.18125267754459884 1104 28-29 0.32399167657431605 1104 30-31 0.24135743792322017 1104 32-33 0.2511692363636584 1104 34-35 0.21487545419904563 1104 36-37 0.22851658736767178 1104 38-39 0.2079729118723037 1104 40-41 0.21067734930958437 1104 42-43 0.23829826317098934 1104 44-45 0.23886226206317218 1104 46-47 0.2292098656780368 1104 48-49 0.21225914761129872 1104 50-51 0.18674963451396565 1104 52-53 0.19743027049882045 1104 54-55 0.2425659479329667 1104 56-57 0.2349754887267821 1104 58-59 0.25329386312676405 1104 60-61 0.1928428710285317 1104 62-63 0.1709055644972679 1104 64-65 0.2080463738474947 1104 66-67 0.1785855302985624 1104 68-69 0.142522564073424 1104 70-71 0.15174977666541878 1104 72-73 0.17098940197638512 1104 74-75 0.15105818801132642 1104 76-77 0.14562077523326877 1104 78-79 0.2931780584033916 1104 80-81 0.2917172977744329 1104 82-83 0.23781280125035664 1104 84-85 0.25026022365106115 1104 86-87 0.3034446024086961 1104 88-89 0.3175019693180765 1104 90-91 0.3390443171722808 1104 92-93 0.27915372065512756 1104 94-95 0.423408255480048 1104 96-97 0.3682188101781314 1104 98-99 0.2896255267776944 1104 100 0.31581187434828095 1105 1 0.06020295269200204 1105 2 0.057677582196149046 1105 3 0.1029451700013837 1105 4 0.04872011987132652 1105 5 0.008069243681525506 1105 6 0.045168257135628664 1105 7 0.0399765881966232 1105 8 0.05052489677120775 1105 9 0.1329239539553413 1105 10-11 0.05690364335671916 1105 12-13 0.11554477965750465 1105 14-15 0.079892482022629 1105 16-17 0.03615364844657876 1105 18-19 0.05443255185235785 1105 20-21 0.016636811051263578 1105 22-23 0.06502769883266524 1105 24-25 0.05593743068450863 1105 26-27 0.1048061117785295 1105 28-29 0.18782362419891996 1105 30-31 0.12621473392078286 1105 32-33 0.08967876428263111 1105 34-35 0.07166415010946992 1105 36-37 0.11850360327694176 1105 38-39 0.1163183480416663 1105 40-41 0.10829908158871149 1105 42-43 0.15769862765226605 1105 44-45 0.11522068698197785 1105 46-47 0.11257270496899707 1105 48-49 0.11599936660817889 1105 50-51 0.13661337249563843 1105 52-53 0.10182258437227176 1105 54-55 0.14636231783563858 1105 56-57 0.09455353217550311 1105 58-59 0.14884589981438268 1105 60-61 0.1186917421035858 1105 62-63 0.1338215667915179 1105 64-65 0.11499333822703761 1105 66-67 0.08129656329133184 1105 68-69 0.04415133559194828 1105 70-71 0.021532750687910607 1105 72-73 0.003986130299438173 1105 74-75 0.04001183190681701 1105 76-77 -0.033707956997712785 1105 78-79 0.169546074125293 1105 80-81 0.1678708474203532 1105 82-83 0.14766855824865388 1105 84-85 0.1600420420501223 1105 86-87 0.19090012612722518 1105 88-89 0.23640815684917982 1105 90-91 0.2321396744894635 1105 92-93 0.20236190591121783 1105 94-95 0.3275765850888881 1105 96-97 0.293750820255152 1105 98-99 0.23093145779695945 1105 100 0.2218028395497882 1106 1 0.024086800925132934 1106 2 0.0285563865089955 1106 3 0.09002814268222892 1106 4 0.03735596859380763 1106 5 -0.03331382606634037 1106 6 -0.004182580824924287 1106 7 -0.008005894396909241 1106 8 0.006971704113254873 1106 9 0.09374429707636267 1106 10-11 0.03541785650050855 1106 12-13 0.11994970351758383 1106 14-15 0.019324867008926105 1106 16-17 0.034846188323257365 1106 18-19 0.02815936764882565 1106 20-21 0.008676118256325083 1106 22-23 2.791204250556234E-4 1106 24-25 0.008757805512054517 1106 26-27 0.06131103287659556 1106 28-29 0.17239317591772618 1106 30-31 0.07262395813119582 1106 32-33 0.12799295051914328 1106 34-35 0.11363420368004995 1106 36-37 0.10202455712692426 1106 38-39 0.10819058005745319 1106 40-41 0.1062539647118328 1106 42-43 0.1305924216929526 1106 44-45 0.11888746594214439 1106 46-47 0.10234464469545657 1106 48-49 0.08617044119000639 1106 50-51 0.12907104546712134 1106 52-53 0.11022658572498756 1106 54-55 0.07490611427122218 1106 56-57 0.06444269412376258 1106 58-59 0.17132537348324917 1106 60-61 0.12685114156597876 1106 62-63 0.11700720533750797 1106 64-65 0.149358590148843 1106 66-67 0.1349444021728985 1106 68-69 0.073377873533083 1106 70-71 0.10030055910871027 1106 72-73 0.07924158911912116 1106 74-75 0.037132026004194074 1106 76-77 0.003012713998913341 1106 78-79 0.12110917798244714 1106 80-81 0.16029562460108338 1106 82-83 0.10850367083939716 1106 84-85 0.10177569093821148 1106 86-87 0.1687441738218851 1106 88-89 0.20579376135041016 1106 90-91 0.23170073558528514 1106 92-93 0.1693607600491731 1106 94-95 0.32439414602448835 1106 96-97 0.2116542336592424 1106 98-99 0.1976514857114644 1106 100 0.22497144081438236 1107 1 -0.018280616991297904 1107 2 -0.04031087350217177 1107 3 0.03373078902298232 1107 4 0.013802679570531495 1107 5 -0.0631571958630559 1107 6 -0.026173271980013624 1107 7 -0.007138792857261933 1107 8 -0.02518537208962357 1107 9 0.030370278604763712 1107 10-11 0.01235436239894483 1107 12-13 0.05388017827987568 1107 14-15 -0.041540277511501245 1107 16-17 -0.02838856495790054 1107 18-19 -0.02109977913568173 1107 20-21 -0.07438291592587376 1107 22-23 -0.0488871505899553 1107 24-25 -0.04713594609216187 1107 26-27 0.018432809068507083 1107 28-29 0.11848086673921898 1107 30-31 0.05320533070679545 1107 32-33 0.006396939361202669 1107 34-35 -0.022536738357537445 1107 36-37 -0.039740404369702276 1107 38-39 -0.018402377192451524 1107 40-41 -0.037004462956630846 1107 42-43 -0.006098668908933291 1107 44-45 -0.03282068695239104 1107 46-47 0.04073642514942577 1107 48-49 -0.005923711172073354 1107 50-51 0.03647745835108651 1107 52-53 -0.02485800900965529 1107 54-55 -0.002720418178341788 1107 56-57 0.010107304906746606 1107 58-59 0.030373695115052612 1107 60-61 -0.05174688816530448 1107 62-63 -0.08075620933750116 1107 64-65 -0.02228355102118229 1107 66-67 -0.024287466660275925 1107 68-69 -0.025171166490373764 1107 70-71 -0.1095757712493608 1107 72-73 -0.06332346570043512 1107 74-75 -0.04962609564342557 1107 76-77 -0.09396765105103455 1107 78-79 0.05137082709574514 1107 80-81 0.09687480412855365 1107 82-83 0.04689730248295376 1107 84-85 0.04270971011830227 1107 86-87 0.08814238115207473 1107 88-89 0.1557676373221213 1107 90-91 0.16863783047192626 1107 92-93 0.08770186734148666 1107 94-95 0.19907177439818113 1107 96-97 0.20628791243267486 1107 98-99 0.13601218811659166 1107 100 0.12645219454909906 1108 1 -0.013906158735672136 1108 2 -0.029656070420891467 1108 3 0.029619247849993258 1108 4 1.510183440487367E-4 1108 5 -0.042005220899724804 1108 6 -0.035249538894916554 1108 7 -0.025993573964846917 1108 8 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-2.273571485324929 2316 50-51 -2.3150121623833826 2316 52-53 -2.225633469683217 2316 54-55 -1.9801369605731907 2316 56-57 -1.9991097985491777 2316 58-59 -1.9082923731334631 2316 60-61 -1.9169056894972982 2316 62-63 -1.9619940402232245 2316 64-65 -1.9176334594679219 2316 66-67 -1.9401202118833112 2316 68-69 -1.8679533188846094 2316 70-71 -1.8154154858237774 2316 72-73 -1.7550383178683688 2316 74-75 -1.7572615163699226 2316 76-77 -1.7682882414811907 2316 78-79 -1.781958115033781 2316 80-81 -1.735426037229061 2316 82-83 -1.7488878246149255 2316 84-85 -1.8817375920889887 2316 86-87 -1.9753365647955086 2316 88-89 -1.989412291550149 2316 90-91 -1.933389520196485 2316 92-93 -2.052103002223621 2316 94-95 -3.900180331765341 2316 96-97 -3.703331764443444 2316 98-99 -2.631674303420869 2316 100 -2.379994522124864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 24.0 9 32.0 10 153.0 11 470.0 12 839.0 13 1609.0 14 3062.0 15 7185.0 16 15768.0 17 31400.0 18 51870.0 19 74537.0 20 99387.0 21 131514.0 22 175450.0 23 237067.0 24 337219.0 25 430816.0 26 447202.0 27 412864.0 28 403512.0 29 437867.0 30 511625.0 31 603834.0 32 714184.0 33 899935.0 34 1217902.0 35 1780758.0 36 3056372.0 37 6173716.0 38 7677837.0 39 2081479.0 40 3552.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.48746286153119 42.796730861136744 17.346000292225423 19.369805985106648 2 21.276768950324918 33.062246681572546 28.713446905071454 16.947537463031086 3 25.30426630198155 31.54345815138334 22.703515138191584 20.448760408443526 4 20.014993017390786 26.238015971139404 28.781575732867054 24.965415278602755 5 20.93459472816216 36.09127482901779 23.735179174623187 19.23895126819686 6 25.406812108785097 24.77660361262771 29.762645531693966 20.053938746893227 7 20.54138668066757 30.60973388429068 28.76919575159298 20.079683683448767 8 24.636473216344115 24.544884908642235 30.501047712569168 20.317594162444482 9 24.658367475530177 29.001831623404183 25.895284272777275 20.44451662828837 10-11 19.966902375229132 30.150534362673998 29.91494892196022 19.96761434013665 12-13 19.95964711379062 27.808398216711698 29.485910660573534 22.746044008924148 14-15 20.024889151167958 32.73318795687984 26.87022831840241 20.371694573549792 16-17 23.13913250341422 27.03212985212114 26.78490851375844 23.043829130706193 18-19 21.05801625378412 29.290495029378633 26.38267332223596 23.268815394601287 20-21 24.220923031989813 29.001356980132503 25.966552138457082 20.8111678494206 22-23 24.52902183087825 26.65056127102188 28.041653266023918 20.77876363207595 24-25 22.178745705057246 29.386958505672904 25.04191001909799 23.39238577017186 26-27 21.724680182327894 27.880376392607303 29.306043238892805 21.088900186171998 28-29 23.547039961189125 26.579850061111323 28.455273758756505 21.417836218943048 30-31 22.6807354135993 26.278613484062653 28.64379841248245 22.396852689855596 32-33 21.563085408642056 27.125412750037942 28.73468708177927 22.57681475954073 34-35 21.938982332473525 26.647433714811903 29.267502871568386 22.14608108114619 36-37 22.10147185201681 25.923156919510298 29.895142767004707 22.08022846146818 38-39 21.74391605949092 26.532319012092266 29.492225721860933 22.231539206555883 40-41 21.808429268088652 27.119686879036887 28.853774061220694 22.218109791653763 42-43 21.260150100521805 26.586176538007873 29.338334090701206 22.81533927076912 44-45 21.206169193415462 26.363916601452907 29.439550079037204 22.990364126094427 46-47 21.74211598567524 26.64477816636103 28.973201246435725 22.63990460152801 48-49 21.64571334640198 26.79812506554353 29.046291626600016 22.50986996145447 50-51 21.19918736979888 27.14752545117204 28.74347961659598 22.909807562433098 52-53 21.161217192043956 26.90229687478948 28.754172751487772 23.182313181678786 54-55 21.89865819478105 27.05377380186033 28.61088929940537 22.436678703953255 56-57 21.930588366766056 27.997733496816235 28.028203620087638 22.04347451633007 58-59 21.508073070586455 28.211869651621967 28.20856639912284 22.071490878668733 60-61 21.66166610781042 27.622068748419572 28.19930628064601 22.516958863124 62-63 21.472155769085948 27.11502160505173 28.40325158972994 23.009571036132385 64-65 22.17494363464992 27.096185972453775 28.348652148916205 22.380218243980103 66-67 22.130691281564292 27.58179052576604 28.220974033736564 22.06654415893311 68-69 22.163508103612507 27.392752954840873 28.01548731749877 22.428251624047853 70-71 22.65378703314438 26.673882056657742 27.60386862965104 23.068462280546832 72-73 22.195269153890365 26.600211762240427 28.143173558527835 23.061345525341377 74-75 21.674965112053638 26.571529694137404 29.11889760727682 22.634607586532134 76-77 21.743057460329194 26.555845823676723 29.113299719936343 22.587796996057747 78-79 22.300549569685256 26.770135210677182 27.846844185139314 23.082471034498255 80-81 22.40871877501134 27.291017999804456 27.854036195332082 22.44622702985212 82-83 22.29038751533429 28.37660708707372 27.559302258477487 21.773703139114502 84-85 22.24682304184241 29.865967449161175 26.172756713851413 21.714452795144997 86-87 21.774048010444925 30.797309415295864 25.799715910282266 21.62892666397694 88-89 21.748802423885383 31.193670789867987 25.510673766134474 21.546853020112156 90-91 21.753701463632744 31.43537810735591 25.211087115194452 21.599833313816895 92-93 21.71519081027773 31.48517740247265 25.048544070474716 21.751087716774904 94-95 21.855604296606312 31.284038156550103 24.904438480236855 21.95591906660673 96-97 21.89781722636173 30.957192906221216 24.941576694431106 22.203413172985943 98-99 22.030403013053636 30.741373109581232 24.868670057547547 22.359553819817588 100 22.145498213778286 30.610941431528456 24.7942770382803 22.44928331641296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8313.0 1 7727.0 2 6394.0 3 5719.0 4 5928.5 5 6254.5 6 6808.0 7 7515.0 8 7797.5 9 8068.5 10 9043.5 11 12918.5 12 72271.0 13 86857.0 14 44595.5 15 47110.5 16 54066.0 17 61251.0 18 68082.0 19 75980.0 20 86000.0 21 99680.0 22 117902.0 23 140653.0 24 165464.5 25 192334.5 26 222282.5 27 259385.0 28 303072.5 29 346527.5 30 390276.0 31 448588.5 32 647860.0 33 825180.0 34 832970.5 35 806510.5 36 769229.0 37 732755.0 38 719269.0 39 730085.0 40 754473.0 41 778930.0 42 804153.5 43 819844.0 44 829623.0 45 849388.0 46 859037.5 47 865656.0 48 873645.5 49 885567.5 50 894514.5 51 892319.0 52 886309.0 53 874631.0 54 852857.5 55 824590.0 56 788469.0 57 739843.5 58 672435.5 59 585720.0 60 486572.5 61 396268.0 62 319842.5 63 248571.0 64 188970.5 65 144047.0 66 108869.0 67 82660.0 68 63554.5 69 49420.0 70 38734.0 71 30401.5 72 24245.5 73 18883.5 74 15166.0 75 13061.0 76 9806.5 77 5879.0 78 3358.5 79 2207.5 80 1427.0 81 917.5 82 594.5 83 363.0 84 237.5 85 146.0 86 86.0 87 64.5 88 40.0 89 23.0 90 17.5 91 12.0 92 9.0 93 5.0 94 2.0 95 2.5 96 1.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07890855172990699 2 0.004357439358790486 3 0.0018700231154842053 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010634864282715519 26-27 0.005440553892281815 28-29 0.007478308088735024 30-31 0.010563489354643603 32-33 0.008641719416307259 34-35 0.003845324249874487 36-37 0.002012772971628038 38-39 0.00190035745991477 40-41 0.001097389519105712 42-43 0.002694403534714838 44-45 0.0036097869872371634 46-47 0.004667920295903322 48-49 0.0019431824167579195 50-51 0.00503550117547369 52-53 0.007892282671552137 54-55 0.008598894459464108 56-57 0.012258643896351614 58-59 0.011434263477120982 60-61 0.008953984726621893 62-63 0.0076157048252734625 64-65 0.009715912083789597 66-67 0.006639652683890008 68-69 0.005254979079294833 70-71 0.006311328014759194 72-73 0.0033546216193800628 74-75 0.004774982688011196 76-77 0.004155805186987323 78-79 0.0036775931689054836 80-81 0.006703890119154732 82-83 0.004735726477571642 84-85 0.007508642433165588 86-87 0.0074176393998738955 88-89 0.010304755240382907 90-91 0.006869836826921937 92-93 0.006839502482491373 94-95 0.0026533629510734863 96-97 0.0019556730291705048 98-99 0.0010759770406841371 100 0.005588656868031041 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8021044E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.25984286510189 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7702710314883 35.0512083176283 2 12.232312581861148 11.31729715827411 3 4.3844893840655725 6.084773698517423 4 2.2202761742050288 4.108385077434169 5 1.303477677557658 3.0149336270992606 6 0.8559802614322515 2.375850743369088 7 0.5882609659688511 1.9049001894574469 8 0.4180129272487887 1.5469769864088185 9 0.32213519279843256 1.3411731060157313 >10 1.6982362210967388 14.097260439384392 >50 0.13032167817362483 4.234034519380611 >100 0.07396745202313225 5.406351064200569 >500 0.0012117477546653905 0.3791790220632119 >1k 7.992802665778566E-4 0.75065660947672 >5k 1.082480259382611E-4 0.38134895512642175 >10k+ 1.39176033343148E-4 8.005670486163963 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 903120 3.223006252015449 No Hit ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC 352325 1.2573585766468944 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp) ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC 339676 1.2122175033878109 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp) ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC 107289 0.38288723289539106 TruSeq Adapter, Index 5 (100% over 35bp) ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG 92555 0.33030532338481033 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT 85082 0.30363608151073884 TruSeq Adapter, Index 25 (97% over 44bp) ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC 70389 0.2512004906027056 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp) ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTG 57538 0.2053385305700958 TruSeq Adapter, Index 5 (97% over 37bp) ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT 52693 0.18804795424467413 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp) ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA 36194 0.12916720733174683 Illumina Multiplexing PCR Primer 2.01 (100% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.03343444305644E-4 1.4274985614383246E-4 0.0 3.568746403595812E-6 7.137492807191624E-6 2 3.247559227272189E-4 1.498873489510241E-4 0.0 3.568746403595812E-6 1.0706239210787435E-5 3 3.60443386763177E-4 1.534560953546199E-4 0.0 7.137492807191624E-6 1.7843732017979058E-5 4 3.747183723775602E-4 1.6059358816181152E-4 0.0 7.137492807191624E-6 2.141247842157487E-5 5 4.7821201808183876E-4 1.7129982737259895E-4 0.0 1.4274985614383248E-5 2.498122482517068E-5 6 5.138994821177969E-4 1.8914355939057804E-4 0.0 1.7843732017979058E-5 2.498122482517068E-5 7 6.495118454544378E-4 1.8914355939057804E-4 0.0 1.7843732017979058E-5 2.498122482517068E-5 8 7.351617591407373E-4 1.9984979860136545E-4 0.0 1.7843732017979058E-5 3.211871763236231E-5 9 0.014464129173773824 2.1055603781215289E-4 0.0 1.7843732017979058E-5 3.5687464035958116E-5 10-11 0.07366427888982295 3.1583405671822937E-4 0.0 1.9628105219776965E-5 4.1040583641351835E-5 12-13 0.3010130529040959 4.7821201808183876E-4 0.0 2.3196851623372773E-5 4.282495684314974E-5 14-15 0.735684580488864 6.191775010238734E-4 0.0 2.498122482517068E-5 4.996244965034136E-5 16-17 1.6742470409025445 0.0011080957583164996 0.0 2.8549971228766496E-5 4.996244965034136E-5 18-19 3.1015029275854245 0.0016344858528468818 0.0 3.0334344430564403E-5 5.353119605393718E-5 20-21 4.035017396211218 0.001991360493206463 0.0 3.211871763236231E-5 5.709994245753299E-5 22-23 4.389090570643978 0.0024874162433062806 0.0 3.211871763236231E-5 6.24530620629267E-5 24-25 4.658869955023803 0.003190459284814656 0.0 3.747183723775602E-5 6.780618166832042E-5 26-27 4.85669627441433 0.0036204932264479513 0.0 3.925621043955393E-5 7.137492807191623E-5 28-29 4.978401233016157 0.0038167742786457204 0.0 4.1040583641351835E-5 8.029679408090576E-5 30-31 5.0468819077547575 0.004196845770628675 0.0 4.282495684314974E-5 8.208116728270367E-5 32-33 5.092254949530075 0.0043538706123868905 0.0 4.282495684314974E-5 8.564991368629948E-5 34-35 5.123518952398776 0.004594760994629608 0.0 4.460933004494765E-5 9.635615289708692E-5 36-37 5.147227919131065 0.004964126247401774 0.0 4.6393703246745554E-5 1.0349364570427855E-4 38-39 5.168176460520172 0.005122935462361788 0.0 4.6393703246745554E-5 1.0706239210787435E-4 40-41 5.190873687647041 0.0054084351746494525 0.0 4.817807644854346E-5 1.0706239210787435E-4 42-43 5.217646423166817 0.005790291039834205 0.0 5.1746822852139266E-5 1.0884676530967225E-4 44-45 5.250518146290338 0.006009768943655347 0.0 5.531556925573509E-5 1.1776863131866179E-4 46-47 5.28952454448164 0.006398762301647291 0.0 5.709994245753299E-5 1.1955300452045969E-4 48-49 5.336610584530684 0.006764558808015861 0.0 6.06686888611288E-5 1.2847487052944923E-4 50-51 5.407382394460392 0.006789540032841032 0.0 6.06686888611288E-5 1.3382799013484292E-4 52-53 5.488899699811327 0.006880543066132725 0.0 6.24530620629267E-5 1.4453422934563036E-4 54-55 5.57030458965055 0.007219573974474328 0.0 6.780618166832042E-5 1.51671722152822E-4 56-57 5.668627121816018 0.007340911352196585 0.0 7.137492807191623E-5 1.6237796136360944E-4 58-59 5.785364385424041 0.0074676018495242365 0.0 7.137492807191623E-5 1.802216933815885E-4 60-61 5.923349965119073 0.007656745408914815 0.0 7.672804767730996E-5 1.8379043978518432E-4 62-63 6.083515303712453 0.007731689083390326 0.0 7.851242087910786E-5 1.9092793259237593E-4 64-65 6.269302457110449 0.007970795092431246 0.0 8.208116728270367E-5 2.08771664610355E-4 66-67 6.487313606159713 0.008065366872126535 0.0 8.564991368629948E-5 2.2126227702294035E-4 68-69 6.738781752742689 0.008343729091607008 0.0 9.10030332916932E-5 2.3018414303192986E-4 70-71 7.026751037541642 0.008795175511661878 0.0 9.278740649349111E-5 2.426747554445152E-4 72-73 7.35360003003457 0.008932572248200318 0.0 9.278740649349111E-5 2.498122482517068E-4 74-75 7.721684102847846 0.009119931434389095 0.0 9.278740649349111E-5 2.5694974105889846E-4 76-77 8.135166198661263 0.009530337270802616 3.568746403595812E-6 9.278740649349111E-5 2.640872338660901E-4 78-79 8.59598771551838 0.009915761882390964 3.568746403595812E-6 9.814052609888481E-5 2.676559802696859E-4 80-81 9.102180489777613 0.010203045967880426 3.568746403595812E-6 9.992489930068272E-5 2.747934730768775E-4 82-83 9.662530775084612 0.01076155478004317 3.568746403595812E-6 1.0527801890607646E-4 2.765778462786754E-4 84-85 10.275673525940004 0.010861479679343852 3.568746403595812E-6 1.0706239210787435E-4 2.8193096588406913E-4 86-87 10.936676734814021 0.01105062323873443 3.568746403595812E-6 1.1063113851147016E-4 2.8728408548946285E-4 88 11.466078851309037 0.011073820090357804 3.568746403595812E-6 1.1419988491506598E-4 2.997746979020482E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 161715 0.0 82.837555 8 GTGGTAT 162435 0.0 82.7134 6 AGTGGTA 163510 0.0 82.24719 5 TGGTATC 162785 0.0 82.15159 7 GTATCAA 165155 0.0 81.56172 9 AAGCAGT 169640 0.0 80.515396 1 GCAGTGG 172105 0.0 78.39277 3 CAGTGGT 172910 0.0 77.93087 4 AGCAGTG 179545 0.0 75.563484 2 CAACGCA 161485 0.0 43.51055 12-13 ATCAACG 162690 0.0 43.24605 10-11 ACGCAGA 165455 0.0 42.627018 14-15 TCAACGC 160600 0.0 41.98579 12-13 AGAGTAC 163220 0.0 41.697918 18-19 AACGCAG 164200 0.0 41.126804 14-15 TATCAAC 166905 0.0 40.399734 10-11 CGCAGAG 166895 0.0 40.272625 16-17 GAGTACT 126805 0.0 39.593918 20-21 AGTACTT 133120 0.0 39.57972 20-21 GCAGAGT 177515 0.0 39.22405 16-17 >>END_MODULE