FastQCFastQC Report
Thu 26 May 2016
SRR1660596_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660596_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24961353
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13560165.432461934254926No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2243000.8985891109348119Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1964430.7869885899213878Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA843190.33779819547441997Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC691910.277192506351719Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG639860.2563402712985951Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG380760.15253980823876015Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC326400.13076214258097307Illumina Single End PCR Primer 1 (100% over 40bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT299670.12005358844129964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2345550.087.72768
GTGGTAT2368200.086.821646
TGGTATC2364900.086.7559057
AGTGGTA2375100.086.685335
GTATCAA2375800.086.517719
AAGCAGT2431250.085.575741
GCAGTGG2458250.084.33753
CAGTGGT2477600.083.2181554
AGCAGTG2525150.082.333032
CAACGCA2320250.045.71082312-13
ATCAACG2335900.045.4519910-11
ACGCAGA2339000.045.32839614-15
AGAGTAC2300950.044.55446618-19
TCAACGC2315200.044.46584712-13
AACGCAG2341400.043.9378914-15
CGCAGAG2356550.043.50300616-17
TATCAAC2376700.043.23472210-11
GAGTACT1740200.042.6870720-21
AGTACTT1806400.042.68122520-21
GCAGAGT2462800.042.6320516-17
Produced by FastQC (version 0.11.5)