FastQCFastQC Report
Thu 26 May 2016
SRR1660596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24961353
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11248284.506278165290159No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2361860.9462067220474787Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2036660.8159253226377592Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA860280.3446447794716897TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG722910.2896117049424364Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT682460.27340665387809704TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC485980.19469297197151134Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT328390.13155937500663525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG322320.1291276157987109TruSeq Adapter, Index 7 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302860.12133156403821539No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1783800.086.0012368
GTATCAA1804550.084.965469
GTGGTAT1806850.084.919726
TGGTATC1805950.084.808527
AGTGGTA1813050.084.7122655
AAGCAGT1871750.083.305531
GCAGTGG1905750.081.462153
CAGTGGT1920000.080.487964
AGCAGTG1974550.078.9597852
CAACGCA1762850.045.46436712-13
ATCAACG1773350.045.05869310-11
ACGCAGA1785300.044.79675714-15
AGAGTAC1774150.043.81689518-19
TCAACGC1762650.043.75253712-13
AACGCAG1778750.043.19288614-15
CGCAGAG1798250.042.58077216-17
TATCAAC1817500.042.21235310-11
AGTACTT1508200.041.7475320-21
GAGTACT1432450.041.74046320-21
GCAGAGT1909850.041.4029116-17
Produced by FastQC (version 0.11.5)