FastQCFastQC Report
Thu 26 May 2016
SRR1660595_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660595_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28153476
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11942844.242048122228318No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG5886252.0907720240299987Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA4826091.7142075102910916Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA1902930.6759129849543267Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1813410.6441158455886584Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1531000.5438049639057003Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1046620.3717551608902574Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC687040.24403380953740847Illumina Single End PCR Primer 1 (100% over 40bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA591760.21019074163346652Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG360240.12795578066452612Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT323920.11505506460374555Illumina Single End PCR Primer 1 (100% over 37bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT284170.10093602651409722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2020600.086.272778
GTGGTAT2038300.085.48876
AGTGGTA2046550.085.3511055
TGGTATC2042050.085.172397
GTATCAA2060400.084.492089
AAGCAGT2132150.082.872581
GCAGTGG2149350.081.873233
CAGTGGT2164400.080.788144
AGCAGTG2229400.079.1716842
CAACGCA1991500.045.26132612-13
ATCAACG2007450.044.94690710-11
ACGCAGA2023750.044.544514-15
AGAGTAC1969650.044.19479418-19
TCAACGC1987700.043.9235312-13
AACGCAG2013550.043.3018114-15
CGCAGAG2036400.042.6968916-17
TATCAAC2044350.042.591410-11
GAGTACT1529250.042.24033720-21
AGTACTT1600750.041.97585320-21
GCAGAGT2132300.041.80607616-17
Produced by FastQC (version 0.11.5)