FastQCFastQC Report
Thu 26 May 2016
SRR1660595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28153476
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11117393.9488516444647903No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3168491.125434742054587Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2515100.8933532754534467Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1077200.38261705233130006Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG970310.34465015971740043TruSeq Adapter, Index 2 (100% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA762380.2707942706612853TruSeq Adapter, Index 22 (100% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC719110.2554249429093587Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT488660.17357004158207676Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG341090.12115377866662007TruSeq Adapter, Index 22 (100% over 39bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA317020.11260421270893868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT285900.10155051546743285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1822550.085.7040948
AGTGGTA1840800.084.9971855
GTGGTAT1841150.084.822386
GTATCAA1847800.084.461049
TGGTATC1846100.084.42647
AAGCAGT1943300.081.628341
GCAGTGG1956800.080.720553
CAGTGGT1969350.079.699024
AGCAGTG2038550.077.7368852
CAACGCA1801050.045.07708412-13
ATCAACG1811300.044.79670310-11
ACGCAGA1829750.044.31470514-15
AGAGTAC1784550.043.95528818-19
TCAACGC1795450.043.65150512-13
AACGCAG1816800.042.9751514-15
CGCAGAG1841200.042.3299316-17
TATCAAC1846950.042.26968810-11
GAGTACT1424400.041.84655820-21
AGTACTT1501900.041.5801420-21
GCAGAGT1938250.041.3390716-17
Produced by FastQC (version 0.11.5)