##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660595_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28153476 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.375419077914216 34.0 31.0 34.0 31.0 34.0 2 32.55530830367092 34.0 31.0 34.0 31.0 34.0 3 32.572273242565146 34.0 31.0 34.0 31.0 34.0 4 36.001348678934 37.0 35.0 37.0 35.0 37.0 5 35.955289322000596 37.0 35.0 37.0 35.0 37.0 6 35.99518649135901 37.0 36.0 37.0 35.0 37.0 7 35.89026424303699 37.0 35.0 37.0 35.0 37.0 8 35.95729415437014 37.0 35.0 37.0 35.0 37.0 9 37.69631842263456 39.0 38.0 39.0 35.0 39.0 10-11 37.57180273228073 39.0 37.5 39.0 35.0 39.0 12-13 37.661367978149485 39.0 38.0 39.0 35.0 39.0 14-15 39.10686520556112 41.0 39.0 41.0 36.0 41.0 16-17 39.03422982298882 40.5 39.0 41.0 36.0 41.0 18-19 38.92096650871814 40.0 38.5 41.0 35.0 41.0 20-21 38.62097081724473 40.0 38.0 41.0 34.0 41.0 22-23 38.38561923934366 40.0 38.0 41.0 33.5 41.0 24-25 38.29985933175712 40.0 38.0 41.0 33.5 41.0 26-27 38.170220899188436 40.0 38.0 41.0 33.0 41.0 28-29 38.062370202528456 40.0 38.0 41.0 33.0 41.0 30-31 37.88827170044651 40.0 38.0 41.0 32.5 41.0 32-33 37.78674698996316 40.0 38.0 41.0 32.0 41.0 34-35 37.90439805727719 40.0 38.0 41.0 32.5 41.0 36-37 37.75083760527474 40.0 38.0 41.0 32.0 41.0 38-39 37.392200646911235 40.0 36.0 41.0 31.0 41.0 40-41 37.30034573705925 40.0 36.0 41.0 31.0 41.0 42-43 37.16605178699781 40.0 36.0 41.0 30.5 41.0 44-45 37.120851133977204 40.0 36.0 41.0 31.0 41.0 46-47 37.005240862620305 40.0 35.0 41.0 30.5 41.0 48-49 36.81182359151673 40.0 35.0 41.0 30.0 41.0 50-51 36.15536857686774 38.5 34.5 40.5 29.0 41.0 52-53 36.00850674708871 39.0 35.0 40.0 28.0 41.0 54-55 35.96141371672897 39.0 35.0 41.0 27.0 41.0 56-57 35.551537508192595 39.0 35.0 41.0 25.5 41.0 58-59 35.225243003741355 38.5 34.5 41.0 24.5 41.0 60-61 35.03790267319034 38.0 34.0 40.5 24.0 41.0 62-63 34.753999541655176 37.5 34.0 40.0 23.5 41.0 64-65 34.44557226255117 37.0 34.0 40.0 23.0 41.0 66-67 34.180441111427946 36.5 33.5 39.5 23.0 41.0 68-69 33.85787619972753 36.0 33.0 39.0 23.0 41.0 70-71 33.55612248377429 35.5 33.0 39.0 23.0 41.0 72-73 33.193370030045315 35.0 33.0 38.5 23.0 40.0 74-75 32.80710745983906 35.0 32.0 37.0 22.0 39.5 76-77 31.824219503126365 34.5 30.5 36.0 20.0 39.0 78-79 32.14248105633564 35.0 32.0 36.0 20.0 39.0 80-81 31.877276930209256 35.0 32.0 36.0 20.0 38.0 82-83 31.443022026836047 35.0 31.5 35.5 18.5 37.0 84-85 30.958327295002576 35.0 31.0 35.0 15.5 37.0 86-87 30.673346996299852 35.0 31.0 35.0 13.5 36.0 88-89 30.34870067198807 35.0 30.5 35.0 10.5 36.0 90-91 30.02667384659713 34.5 30.0 35.0 10.0 36.0 92-93 29.65633577537637 34.0 29.0 35.0 10.0 35.5 94-95 29.305276478115882 34.0 29.0 35.0 9.0 35.0 96-97 29.043852631199073 34.0 28.5 35.0 8.0 35.0 98-99 28.81890708628661 34.0 27.0 35.0 7.5 35.0 100 28.665256894033263 34.0 27.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24773917292466052 1101 2 0.25629219834946326 1101 3 0.2527460771121497 1101 4 0.22207338960627965 1101 5 0.1929765354349584 1101 6 0.16933228338781703 1101 7 0.19447461186132387 1101 8 0.1952732012808056 1101 9 0.2746133754294533 1101 10-11 0.27534448732912864 1101 12-13 0.2914330092044537 1101 14-15 0.3331514778374185 1101 16-17 0.3206044201229048 1101 18-19 0.3296359633522741 1101 20-21 0.4024020051818127 1101 22-23 0.36929980081988134 1101 24-25 0.2678042287412268 1101 26-27 0.30859477341886077 1101 28-29 0.33877420405783454 1101 30-31 0.3546122324239249 1101 32-33 0.34789503563396096 1101 34-35 0.343750497249232 1101 36-37 0.32372055444316317 1101 38-39 0.2694072747753893 1101 40-41 0.2920847474802031 1101 42-43 0.37649721928485747 1101 44-45 0.3750066422462339 1101 46-47 0.37650188915549165 1101 48-49 0.33360691366303286 1101 50-51 0.35165375987291014 1101 52-53 0.3837982050383957 1101 54-55 0.40718873572481584 1101 56-57 0.4165514357238891 1101 58-59 0.35565664011488707 1101 60-61 0.3196287626066052 1101 62-63 0.2627928350026494 1101 64-65 0.2320163114028304 1101 66-67 0.25643102569051734 1101 68-69 0.24899673263022493 1101 70-71 0.16180161069144106 1101 72-73 0.13983579561080717 1101 74-75 0.1143266171075652 1101 76-77 0.03968300640943312 1101 78-79 0.10758246749394118 1101 80-81 0.0873307099491889 1101 82-83 0.0884902988000178 1101 84-85 0.03372244568206284 1101 86-87 -0.029735916814164653 1101 88-89 -0.049618976226032174 1101 90-91 -0.09288099510216696 1101 92-93 -0.10696165529708779 1101 94-95 -0.08157519627471288 1101 96-97 -0.09672006565257618 1101 98-99 -0.00744620221493264 1101 100 0.006955914359370041 1102 1 0.15971052903393712 1102 2 0.17678581534051574 1102 3 0.19895902302944535 1102 4 0.14742940994014475 1102 5 0.10833926569820562 1102 6 0.1659953328685404 1102 7 0.19100511638345807 1102 8 0.18657734762765443 1102 9 0.23919414244581105 1102 10-11 0.21447596989928286 1102 12-13 0.24176051321635583 1102 14-15 0.23544507230842981 1102 16-17 0.23239638344346503 1102 18-19 0.24564852023503647 1102 20-21 0.353696143245088 1102 22-23 0.32488654003504536 1102 24-25 0.23557154442129047 1102 26-27 0.2784076062602807 1102 28-29 0.3146835125103209 1102 30-31 0.29026120234674835 1102 32-33 0.3044365142881844 1102 34-35 0.25701857829609054 1102 36-37 0.22759638532206594 1102 38-39 0.25992607464285555 1102 40-41 0.24832407019542302 1102 42-43 0.3245523272650104 1102 44-45 0.29558314955678355 1102 46-47 0.2386242090646391 1102 48-49 0.3037960661084611 1102 50-51 0.2874595799058852 1102 52-53 0.3626305241789254 1102 54-55 0.40954670489612965 1102 56-57 0.3356552578865504 1102 58-59 0.3873172371868492 1102 60-61 0.3060651790810951 1102 62-63 0.2472087259830218 1102 64-65 0.259528644540552 1102 66-67 0.32041719057381357 1102 68-69 0.28407753834693494 1102 70-71 0.21812131349196306 1102 72-73 0.23250284484340256 1102 74-75 0.15770701712945367 1102 76-77 0.07849865049179527 1102 78-79 0.19039775540032622 1102 80-81 0.14784353486680502 1102 82-83 0.12891267749836643 1102 84-85 0.14337408926809303 1102 86-87 0.02670048449872553 1102 88-89 0.08209135986403382 1102 90-91 -0.0013796811186388425 1102 92-93 -0.015092670142866638 1102 94-95 0.009543829163003892 1102 96-97 0.015865798382407093 1102 98-99 0.05328376886105346 1102 100 -0.006335689960092594 1103 1 0.16070816785535413 1103 2 0.14635369353187144 1103 3 0.18513590357518694 1103 4 0.16114622793911337 1103 5 0.15609402405903694 1103 6 0.143815537266633 1103 7 0.16986611402670349 1103 8 0.1367739013353031 1103 9 0.16947672409821735 1103 10-11 0.1841041479620671 1103 12-13 0.213008170221471 1103 14-15 0.24654888466321268 1103 16-17 0.25800891766073875 1103 18-19 0.23303013405102746 1103 20-21 0.33928157794548497 1103 22-23 0.21409420795992418 1103 24-25 0.23281373336644862 1103 26-27 0.2032607713067165 1103 28-29 0.20767208731975728 1103 30-31 0.16688845369976946 1103 32-33 0.24422195512063638 1103 34-35 0.19502253004539938 1103 36-37 0.14049171334603017 1103 38-39 0.21842398835863008 1103 40-41 0.15380094545189138 1103 42-43 0.22223690817563124 1103 44-45 0.21451137116621766 1103 46-47 0.20250481119186503 1103 48-49 0.2127694326542411 1103 50-51 0.20624584967371362 1103 52-53 0.26483439339295245 1103 54-55 0.26350849553291056 1103 56-57 0.28546342097952504 1103 58-59 0.22170438614489996 1103 60-61 0.22978416419030623 1103 62-63 0.15625179267512124 1103 64-65 0.10005364747407697 1103 66-67 0.09942086234329395 1103 68-69 0.11507764745228855 1103 70-71 0.08461035475179557 1103 72-73 -0.002493647493771789 1103 74-75 0.007496675924691942 1103 76-77 -0.0502508959733845 1103 78-79 0.052990381915059004 1103 80-81 0.05506667661510889 1103 82-83 0.06926519748438764 1103 84-85 0.10185462681525692 1103 86-87 0.07761603602446954 1103 88-89 0.06542465004401166 1103 90-91 0.0036910053275356347 1103 92-93 -0.07248597240839061 1103 94-95 0.06652013222199571 1103 96-97 0.008854184289095457 1103 98-99 0.11996350447872928 1103 100 0.09769147720927762 1104 1 0.1483523966825686 1104 2 0.16102287083717215 1104 3 0.16655690748635976 1104 4 0.14583596706136603 1104 5 0.08602157355856122 1104 6 0.13714122361495384 1104 7 0.14587462160928766 1104 8 0.11899784600785068 1104 9 0.18794873232841525 1104 10-11 0.17492659838919877 1104 12-13 0.2014173359523781 1104 14-15 0.21843283057691565 1104 16-17 0.19021273746594858 1104 18-19 0.15784556868605648 1104 20-21 0.30973409514203354 1104 22-23 0.24290094257848693 1104 24-25 0.22356971830467387 1104 26-27 0.18836026683653984 1104 28-29 0.18655510962419442 1104 30-31 0.20949550953375962 1104 32-33 0.1939198730496159 1104 34-35 0.16085143507656596 1104 36-37 0.14100961294904124 1104 38-39 0.15802436932881392 1104 40-41 0.20660044702613334 1104 42-43 0.21039918637174537 1104 44-45 0.20019477943384345 1104 46-47 0.19425345359161383 1104 48-49 0.16424531922181984 1104 50-51 0.17057716003058232 1104 52-53 0.22726810273280051 1104 54-55 0.1726642807604577 1104 56-57 0.15594595309349302 1104 58-59 0.20711360975268178 1104 60-61 0.11351418273144276 1104 62-63 0.1173098809572295 1104 64-65 0.0892268056666552 1104 66-67 0.08603081407490976 1104 68-69 0.07412208143798438 1104 70-71 0.10710260812662398 1104 72-73 0.0950747193494621 1104 74-75 0.05996137127618795 1104 76-77 -0.007419237157137104 1104 78-79 0.06622800213244773 1104 80-81 0.023628060235747483 1104 82-83 0.07068861525487335 1104 84-85 0.07199128775829777 1104 86-87 0.05524963795344462 1104 88-89 -0.05973498205174721 1104 90-91 -0.006132056608986858 1104 92-93 -0.09205123187234676 1104 94-95 0.014232695494108327 1104 96-97 0.0720181881549884 1104 98-99 0.06963517670030583 1104 100 0.04403900389841198 1105 1 0.10577721987465338 1105 2 0.11510766135710071 1105 3 0.12355159658353188 1105 4 0.11198352935594613 1105 5 0.0839667927019363 1105 6 0.10131015508975594 1105 7 0.11539407490818832 1105 8 0.12695183679647926 1105 9 0.1432345143859166 1105 10-11 0.14811573769498665 1105 12-13 0.13815888857693182 1105 14-15 0.187858369381253 1105 16-17 0.17357668695947126 1105 18-19 0.19325581357990984 1105 20-21 0.25118168508601 1105 22-23 0.20049122168078526 1105 24-25 0.19791524282192796 1105 26-27 0.2105130350202984 1105 28-29 0.1714460217541145 1105 30-31 0.17160814530805624 1105 32-33 0.16533557296622092 1105 34-35 0.16413340844365365 1105 36-37 0.1663653786965611 1105 38-39 0.1795978961687723 1105 40-41 0.22287975762394296 1105 42-43 0.24977670252743422 1105 44-45 0.28140162153154336 1105 46-47 0.250263611855047 1105 48-49 0.22383109168686843 1105 50-51 0.24579820138927033 1105 52-53 0.2984230933556873 1105 54-55 0.2660388267034861 1105 56-57 0.25912368888756276 1105 58-59 0.2689273679936619 1105 60-61 0.22524040979694604 1105 62-63 0.1877258608169896 1105 64-65 0.21482867062125877 1105 66-67 0.1771269066353156 1105 68-69 0.2194662600280992 1105 70-71 0.21150485049642498 1105 72-73 0.18104434714906148 1105 74-75 0.14580598949731183 1105 76-77 0.05884156670945373 1105 78-79 0.16085450245713417 1105 80-81 0.1972004224171151 1105 82-83 0.16384084037875724 1105 84-85 0.1475046065920118 1105 86-87 0.128193694162821 1105 88-89 0.08021095713188586 1105 90-91 0.09464545565938565 1105 92-93 0.11849873173411041 1105 94-95 0.1974186240300213 1105 96-97 0.21091428808919588 1105 98-99 0.21999108227957365 1105 100 0.0999535696863596 1106 1 0.055722234738219356 1106 2 0.11133984751593573 1106 3 0.11362420824232089 1106 4 0.09415405988519865 1106 5 0.06718895924884549 1106 6 0.10400482880338302 1106 7 0.09064959277221618 1106 8 0.09608612749971002 1106 9 0.13714403578284617 1106 10-11 0.10762465296539148 1106 12-13 0.12902822364114286 1106 14-15 0.15143852512800038 1106 16-17 0.12220755993399735 1106 18-19 0.12394348939009348 1106 20-21 0.18173046776121282 1106 22-23 0.1429145749364764 1106 24-25 0.11072492409368806 1106 26-27 0.1281919392742381 1106 28-29 0.1311187305270991 1106 30-31 0.09167980976989298 1106 32-33 0.13324974013446678 1106 34-35 0.13135225396961658 1106 36-37 0.13953741117799723 1106 38-39 0.13428708546184254 1106 40-41 0.19647332919615934 1106 42-43 0.17270646420666935 1106 44-45 0.1719471380560691 1106 46-47 0.16987693062817755 1106 48-49 0.14488545641813033 1106 50-51 0.19396364347401374 1106 52-53 0.18624287283374485 1106 54-55 0.15604420019066367 1106 56-57 0.10617876123325942 1106 58-59 0.16056108967185878 1106 60-61 0.14885677881815695 1106 62-63 0.14981404882922078 1106 64-65 0.14291349869088066 1106 66-67 0.12055946753778812 1106 68-69 0.17297515312066736 1106 70-71 0.13851852096943418 1106 72-73 0.05623484683432878 1106 74-75 0.08612640205989663 1106 76-77 -0.03218926786472309 1106 78-79 0.05728502123124457 1106 80-81 0.1167647853776792 1106 82-83 0.10299065402305985 1106 84-85 0.13016045958881506 1106 86-87 0.12301301501907247 1106 88-89 0.03598086876688811 1106 90-91 0.04877219153916812 1106 92-93 0.0019114664436834516 1106 94-95 0.019890826406069095 1106 96-97 0.07291617470759704 1106 98-99 0.10014884358665199 1106 100 0.0821152423336784 1107 1 -0.012167091604730729 1107 2 0.018300420537052275 1107 3 0.02080660656668698 1107 4 0.026399667265359028 1107 5 -0.003899098721575456 1107 6 0.035666450876306044 1107 7 0.07171150122842818 1107 8 0.022572294318081276 1107 9 0.08496926272383121 1107 10-11 0.10469021623213592 1107 12-13 0.04992725922743091 1107 14-15 0.08490218706656094 1107 16-17 0.027028754837452595 1107 18-19 0.06609327293553235 1107 20-21 0.1154866975548714 1107 22-23 0.0485596792473828 1107 24-25 0.08199926714654282 1107 26-27 0.04966086806000902 1107 28-29 0.03934197204872447 1107 30-31 -0.007363423083866394 1107 32-33 0.028495144360043412 1107 34-35 -0.023998078314100724 1107 36-37 0.015461294548224203 1107 38-39 0.004959349012089831 1107 40-41 0.010670882232496126 1107 42-43 0.05652682009166199 1107 44-45 0.06376381551768162 1107 46-47 0.047298936461864116 1107 48-49 0.03835210559166313 1107 50-51 0.020346548806593034 1107 52-53 0.09892496058874656 1107 54-55 0.12230294272737297 1107 56-57 0.10010620201761355 1107 58-59 0.11353396629880308 1107 60-61 0.06341946252765496 1107 62-63 0.06478055356472368 1107 64-65 0.07931636068837378 1107 66-67 0.0719692318216616 1107 68-69 0.10886428590666952 1107 70-71 0.12567636745924204 1107 72-73 0.06756887989315175 1107 74-75 0.022639282024194074 1107 76-77 -0.023082654906666278 1107 78-79 0.07890665845215494 1107 80-81 0.09952329696606554 1107 82-83 0.08142341771540629 1107 84-85 0.0964278248888526 1107 86-87 0.05649436818326947 1107 88-89 0.035438479401737055 1107 90-91 0.009735757369778497 1107 92-93 0.03182740261980044 1107 94-95 -0.004815363960982921 1107 96-97 0.0050919467108236915 1107 98-99 0.038291672992119885 1107 100 0.04143736784082819 1108 1 -0.07090592624869885 1108 2 -0.04748343001459432 1108 3 -0.028838599985959945 1108 4 0.013924736664066018 1108 5 -0.06328474801352968 1108 6 -0.013458635292586507 1108 7 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-0.7533065772556178 2316 82-83 -0.7452889121197153 2316 84-85 -0.5810855679839975 2316 86-87 -0.6310544291279356 2316 88-89 -0.6123776300200277 2316 90-91 -0.6027889011649883 2316 92-93 -0.5835756825617118 2316 94-95 -0.8911851534636561 2316 96-97 -0.9560401942888994 2316 98-99 -1.6973002906684584 2316 100 -1.7808312851262862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 880.0 3 873.0 4 759.0 5 998.0 6 1709.0 7 1132.0 8 1793.0 9 4808.0 10 7611.0 11 8096.0 12 10732.0 13 14953.0 14 20782.0 15 28923.0 16 43950.0 17 67769.0 18 98310.0 19 133325.0 20 166543.0 21 196810.0 22 239341.0 23 310317.0 24 427380.0 25 548933.0 26 580478.0 27 536512.0 28 519324.0 29 561811.0 30 655613.0 31 722221.0 32 756036.0 33 915709.0 34 1239612.0 35 1759106.0 36 3013603.0 37 5856680.0 38 6835385.0 39 1860986.0 40 3673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.543878884200936 47.488451269755245 14.035345296878523 19.9323245491653 2 20.268393406996633 31.5381509650029 32.962778530744416 15.230677097256052 3 24.865059407112213 34.11982469579373 21.32937945498278 19.685736442111278 4 18.596419681556064 24.609398007318646 31.760056213123907 25.034126098001387 5 19.71716977525499 39.797884123058175 22.40815838054944 18.076787721137393 6 25.09188001253118 23.216532750505646 32.7349642225755 18.956623014387663 7 19.64788431697566 33.2230158826579 28.167474927157798 18.961624873208642 8 24.240768823280614 22.771640791294313 33.65971505695142 19.327875328473656 9 24.30680169610647 31.772030867051914 24.604438017854058 19.316729418987556 10-11 18.949108945705472 31.08048295531189 31.12759662204583 18.842811476936816 12-13 19.175381082386817 28.258943829385892 30.525120256903282 22.04055483132401 14-15 19.396171007919232 33.93645972286485 27.367756382618452 19.299612886597462 16-17 23.010226381265888 27.30042070389468 27.101357909632494 22.587995005206935 18-19 20.999639170348296 29.970408913391182 26.203542027773118 22.826409888487404 20-21 24.773689805078565 29.66585650522437 25.34026274258808 20.220190947108982 22-23 25.461183304724834 26.19975917406255 27.868232793289504 20.470824727923116 24-25 23.22515277846225 29.686065145326836 23.84493804634514 23.24384402986578 26-27 22.263738629165136 28.221997113933124 29.060027447908737 20.454236808993 28-29 24.46785938874641 26.169384741512186 28.23548866143352 21.127267208307877 30-31 24.186410503263307 25.213207852539878 28.639444301035226 21.960937343161593 32-33 23.17697029448425 25.72423557069988 29.049772975831083 22.04902115898479 34-35 23.489931931960744 25.461804983672803 29.217120717142304 21.83114236722415 36-37 23.24585043541855 25.361827426193663 29.979000870721606 21.413321267666184 38-39 23.1396328806118 25.91294410717425 29.45413574965646 21.493287262557494 40-41 23.79083760597753 26.459722089270244 28.482417329158373 21.267022975593854 42-43 22.734464732243982 26.772100253609832 28.817257904765288 21.676177109380898 44-45 22.452447436105448 26.67491370440988 29.12654635066892 21.746092508815757 46-47 23.02064906796688 26.270729026469823 29.1275847756452 21.581037129918094 48-49 23.081708347414967 25.611909254947925 29.684669589345265 21.621712808291843 50-51 22.56347224770828 25.858139657849254 29.239749073156368 22.338639021286095 52-53 22.717208453975356 25.8779197341646 29.022439696045264 22.38243211581478 54-55 22.701490570635958 25.98043396389076 29.081569558577108 22.236505906896177 56-57 23.003808648899344 26.048869206134327 28.068705253581538 22.878616891384787 58-59 23.732221131346165 26.436811549833607 27.518020850944332 22.312946467875904 60-61 23.676803840220085 26.562498306487093 27.344276279957718 22.416421573335104 62-63 23.304574301060672 25.791645405089003 27.79402449856125 23.10975579528908 64-65 23.13101521241865 25.72066261750056 27.88939451002184 23.25892766005895 66-67 23.466271204657545 25.872436707697393 27.691571189105087 22.969720898539975 68-69 22.80548280137948 26.147170666795493 28.291606780655055 22.75573975116997 70-71 22.545686055394775 25.92766592457195 28.58175221741287 22.944895802620398 72-73 22.02943767232193 26.0691851240048 28.883015311323213 23.018361892350057 74-75 21.431166058821336 26.872385071633083 29.593553462486405 22.10289540705918 76-77 21.342842352435916 27.732311938081818 29.168811608186274 21.756034101295995 78-79 21.675086529316694 28.83867695738352 27.528327392235223 21.957909121064567 80-81 21.793954644687517 29.70041705309429 27.1121896189201 21.39343868329809 82-83 21.69127325676515 30.68203188289356 26.779049720511626 20.84764513982966 84-85 21.89012770357988 31.779099237504475 25.490763490271906 20.840009568643737 86-87 21.52237657316851 32.55363839267271 25.19913451798546 20.724850516173323 88-89 21.715895272178436 32.638945641541135 24.93105674478017 20.714102341500258 90-91 21.8893307988726 32.53722897917301 24.679792964070877 20.89364725788351 92-93 22.04972517469913 32.324627953968374 24.619637479635486 21.006009391697006 94-95 22.393136668030184 31.938073705298976 24.547182599340243 21.1216070273306 96-97 22.648713678872543 31.239685103770004 24.62092992371977 21.490671293637682 98-99 22.94562922574214 30.777740206542592 24.662882476378638 21.613748091336628 100 23.221538186714362 30.50557625125202 24.538803835541596 21.734081726492022 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3073.0 1 3406.5 2 3430.0 3 3108.5 4 3319.5 5 3741.0 6 4204.0 7 4548.0 8 4789.0 9 5057.0 10 5408.0 11 7444.0 12 61447.5 13 79063.0 14 45930.5 15 51538.5 16 60014.0 17 67856.5 18 75126.0 19 82151.5 20 89448.0 21 99647.0 22 114065.0 23 132298.5 24 152856.5 25 174694.5 26 198955.0 27 229231.0 28 266544.5 29 306195.5 30 354425.0 31 421916.5 32 505706.5 33 599562.5 34 810880.0 35 1005907.5 36 1030500.5 37 981805.0 38 911442.0 39 852435.5 40 811103.0 41 789664.5 42 784707.0 43 784401.5 44 795688.0 45 823082.5 46 845662.5 47 866246.0 48 877648.5 49 882509.5 50 883134.5 51 882275.0 52 886990.0 53 875933.0 54 846203.0 55 814833.0 56 775841.5 57 719286.0 58 647258.0 59 567240.5 60 477827.0 61 390989.5 62 314645.0 63 243669.0 64 184666.5 65 139745.0 66 103748.0 67 76739.5 68 57915.5 69 44373.5 70 34577.0 71 26866.0 72 21350.5 73 16670.0 74 12973.5 75 10736.5 76 8267.5 77 5412.0 78 3482.0 79 2389.5 80 1593.5 81 1113.0 82 778.5 83 499.5 84 324.0 85 223.5 86 157.0 87 101.5 88 63.5 89 52.5 90 50.5 91 41.0 92 25.0 93 17.0 94 13.5 95 8.0 96 5.5 97 7.0 98 7.5 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05979013035548435 2 0.02497737757142315 3 0.015049651417821372 4 0.012311090822319775 5 0.009217334300034569 6 0.010311337754528074 7 0.010293577958188893 8 0.005949531773625396 9 0.0035448553493003846 10-11 0.004786265113409087 12-13 0.007748599142784358 14-15 0.003559063186371729 16-17 0.009093015725660306 18-19 0.015838186375280977 20-21 0.0322713259279245 22-23 0.0366846353182108 24-25 0.03300835747600048 26-27 0.03546276133007519 28-29 0.03267624928445781 30-31 0.03360508663299694 32-33 0.041374997531388305 34-35 0.04184563213437659 36-37 0.04625894152466289 38-39 0.04002525300961061 40-41 0.037231637045457545 42-43 0.03499035074745299 44-45 0.03995954176315564 46-47 0.03819421800704112 48-49 0.039167454846428196 50-51 0.04165737829318127 52-53 0.04209604526275903 54-55 0.046097327377976345 56-57 0.04775431637642187 58-59 0.047963881973224196 60-61 0.04619500625784184 62-63 0.045910849516414956 64-65 0.042486760782220995 66-67 0.04697643729676577 68-69 0.04568352412327345 70-71 0.05286913772210579 72-73 0.05137731482961464 74-75 0.06063016872232757 76-77 0.058747630310374464 78-79 0.05135422709437371 80-81 0.048922910975539934 82-83 0.05584567958855241 84-85 0.05589718299793603 86-87 0.054694844785773515 88-89 0.0569183712874389 90-91 0.05869257494172301 92-93 0.058967851784980305 94-95 0.06782288623969558 96-97 0.061400943883447995 98-99 0.06142758357795677 100 0.06392461094324552 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8153476E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.193448558855316 #Duplication Level Percentage of deduplicated Percentage of total 1 69.49469637716898 27.237368075718404 2 14.084583635637948 11.040468083925425 3 5.5754938266831005 6.555684914589584 4 2.9620411595144636 4.643704312585691 5 1.8802112877278585 3.684598219267047 6 1.189182993235691 2.7964909483469187 7 0.8449268421678878 2.318091770715222 8 0.6307949510200006 1.9778423571190438 9 0.47845576317353344 1.6877098207466765 >10 2.5775155196706225 18.32018856239203 >50 0.20387915400422665 5.519224332818441 >100 0.0757565473972291 4.523539144691074 >500 0.0014060287860462033 0.38170129079246673 >1k 7.712995977001932E-4 0.6056626393275971 >5k 1.4230710734748915E-4 0.38938058754838806 >10k+ 1.423071073427251E-4 8.31834493941599 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1111739 3.9488516444647903 No Hit ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC 316849 1.125434742054587 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp) ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC 251510 0.8933532754534467 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp) ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG 107720 0.38261705233130006 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp) ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG 97031 0.34465015971740043 TruSeq Adapter, Index 2 (100% over 35bp) ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA 76238 0.2707942706612853 TruSeq Adapter, Index 22 (100% over 37bp) ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC 71911 0.2554249429093587 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp) ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT 48866 0.17357004158207676 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp) ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG 34109 0.12115377866662007 TruSeq Adapter, Index 22 (100% over 39bp) ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA 31702 0.11260421270893868 Illumina Multiplexing PCR Primer 2.01 (100% over 24bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 28590 0.10155051546743285 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.308773524093437E-4 7.103918535672114E-6 0.0 0.0 7.103918535672114E-6 2 2.9126065996255666E-4 1.7759796339180284E-5 0.0 0.0 7.103918535672114E-6 3 3.3743613044442543E-4 2.8415674142688455E-5 0.0 3.551959267836057E-6 1.065587780350817E-5 4 3.658518045871139E-4 3.9071551946196626E-5 0.0 3.551959267836057E-6 1.065587780350817E-5 5 5.576576050502609E-4 4.617547048186874E-5 0.0 7.103918535672114E-6 1.065587780350817E-5 6 6.18040912603474E-4 6.038330755321297E-5 0.0 7.103918535672114E-6 1.4207837071344227E-5 7 7.849829981917685E-4 6.393526682104902E-5 0.0 7.103918535672114E-6 1.4207837071344227E-5 8 8.915417762268503E-4 7.45911446245572E-5 0.0 1.065587780350817E-5 1.4207837071344227E-5 9 0.030177445939535138 7.814310389239325E-5 0.0 1.065587780350817E-5 1.4207837071344227E-5 10-11 0.17611679637711522 8.347104279414734E-5 0.0 1.065587780350817E-5 1.9535775973098313E-5 12-13 0.7392657304554506 9.412692059765551E-5 0.0 1.4207837071344227E-5 2.131175560701634E-5 14-15 1.6349810588220084 9.590290023157354E-5 0.0 1.7759796339180284E-5 2.6639694508770427E-5 16-17 3.104600653929909 1.0478279840116368E-4 0.0 1.7759796339180284E-5 3.0191653776606483E-5 18-19 5.382754513154966 1.24318574374262E-4 0.0 1.7759796339180284E-5 3.374361304444254E-5 20-21 7.063616229839612 1.4740630961519637E-4 0.0 1.7759796339180284E-5 3.72955723122786E-5 22-23 7.778892027400097 1.545102281508685E-4 0.0 1.7759796339180284E-5 4.0847531580114654E-5 24-25 8.260953283353004 1.7582198375788483E-4 0.0 1.7759796339180284E-5 4.617547048186874E-5 26-27 8.572477870938565 1.8825384119531101E-4 0.0 1.9535775973098313E-5 4.617547048186874E-5 28-29 8.751132542212549 2.0246167826665524E-4 0.0 2.308773524093437E-5 4.617547048186874E-5 30-31 8.847363998676398 2.2199745423975354E-4 0.0 2.4863714874852398E-5 4.617547048186874E-5 32-33 8.902366087938839 2.3265333204326174E-4 0.0 2.8415674142688455E-5 4.9727429749704796E-5 34-35 8.934292873817784 2.4686116911460597E-4 0.0 2.8415674142688455E-5 4.9727429749704796E-5 36-37 8.951784497232243 2.557410672841961E-4 0.0 3.196763341052451E-5 4.9727429749704796E-5 38-39 8.965564323211812 2.699489043555403E-4 0.0 3.374361304444254E-5 5.327938901754085E-5 40-41 8.980400857073564 2.8593272106080256E-4 0.0 4.262351121403268E-5 5.327938901754085E-5 42-43 8.99811802990153 3.054684970339009E-4 0.0 5.1503409383622825E-5 5.327938901754085E-5 44-45 9.019071037622496 3.214523137391632E-4 0.0 5.505536865145888E-5 5.327938901754085E-5 46-47 9.043979151988195 3.4986798788185164E-4 0.0 5.683134828537691E-5 5.327938901754085E-5 48-49 9.074161925866632 3.569719064175237E-4 0.0 5.683134828537691E-5 5.683134828537691E-5 50-51 9.119069346889884 3.676277842210319E-4 0.0 5.860732791929494E-5 6.038330755321297E-5 52-53 9.171613480338982 3.818356212923761E-4 0.0 6.038330755321297E-5 6.393526682104902E-5 54-55 9.22435652350708 4.013713972654744E-4 0.0 6.038330755321297E-5 6.393526682104902E-5 56-57 9.287116091810475 4.33339030675999E-4 0.0 6.2159287187131E-5 6.926320572280311E-5 58-59 9.361256137608017 4.564267659169333E-4 0.0 6.748722608888508E-5 7.45911446245572E-5 60-61 9.449087210403434 4.777385215239497E-4 0.0 6.926320572280311E-5 7.991908352631128E-5 62-63 9.552015886066787 4.8306646042570373E-4 0.0 7.103918535672114E-5 8.524702242806536E-5 64-65 9.672594247332018 4.9549831786313E-4 0.0 7.45911446245572E-5 9.235094096373748E-5 66-67 9.813006749148844 5.097061549344742E-4 0.0 7.45911446245572E-5 1.0123083913332762E-4 68-69 9.974365154768101 5.221380123719004E-4 0.0 7.45911446245572E-5 1.065587780350817E-4 70-71 10.161491248895873 5.523296661485069E-4 0.0 7.636712425847522E-5 1.065587780350817E-4 72-73 10.373996091992336 5.878492588268674E-4 0.0 8.169506316022931E-5 1.065587780350817E-4 74-75 10.615900501948676 6.144889533356379E-4 0.0 8.70230020619834E-5 1.065587780350817E-4 76-77 10.887350109094877 6.304727700409002E-4 0.0 9.057496132981945E-5 1.1543867620467185E-4 78-79 11.192868333558527 6.446806071122443E-4 0.0 9.412692059765551E-5 1.189906354725079E-4 80-81 11.533124719661615 6.517845256479164E-4 0.0 9.590290023157354E-5 1.2076661510642593E-4 82-83 11.907728551884677 6.677683423531787E-4 0.0 9.590290023157354E-5 1.24318574374262E-4 84-85 12.320153646391656 6.85528138692359E-4 0.0 9.590290023157354E-5 1.2609455400818003E-4 86-87 12.770407107101091 7.032879350315393E-4 0.0 9.590290023157354E-5 1.2787053364209805E-4 88 13.132737144074145 7.103918535672114E-4 0.0 9.945485949940959E-5 1.2787053364209805E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 182255 0.0 85.704094 8 AGTGGTA 184080 0.0 84.997185 5 GTGGTAT 184115 0.0 84.82238 6 GTATCAA 184780 0.0 84.46104 9 TGGTATC 184610 0.0 84.4264 7 AAGCAGT 194330 0.0 81.62834 1 GCAGTGG 195680 0.0 80.72055 3 CAGTGGT 196935 0.0 79.69902 4 AGCAGTG 203855 0.0 77.736885 2 CAACGCA 180105 0.0 45.077084 12-13 ATCAACG 181130 0.0 44.796703 10-11 ACGCAGA 182975 0.0 44.314705 14-15 AGAGTAC 178455 0.0 43.955288 18-19 TCAACGC 179545 0.0 43.651505 12-13 AACGCAG 181680 0.0 42.97515 14-15 CGCAGAG 184120 0.0 42.32993 16-17 TATCAAC 184695 0.0 42.269688 10-11 GAGTACT 142440 0.0 41.846558 20-21 AGTACTT 150190 0.0 41.58014 20-21 GCAGAGT 193825 0.0 41.33907 16-17 >>END_MODULE